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The map label for this gene is ansA [H]

Identifier: 38234162

GI number: 38234162

Start: 1614825

End: 1615745

Strand: Direct

Name: ansA [H]

Synonym: DIP1587

Alternate gene names: 38234162

Gene position: 1614825-1615745 (Clockwise)

Preceding gene: 38234160

Following gene: 38234185

Centisome position: 64.89

GC content: 54.18

Gene sequence:

>921_bases
TTGCCCAAAGTCATAGTCCTTACTACCGGCGGCACACTATCGTGCACCGCAGACCCCAATGGGGCACTTATCCCTACTGT
TTCTGGCGACGAACTTGTTGCACCAATTCGTCAGCGCTTTGCCGGAGCGCTCGATATTGAGGTTTGTGAATTACATCGAC
TTGATTCTTCGTCGATGACTCTGCGCGATATTGATGACATTGTCACAGCAACGCACAATGCTTTAGAAGATGACTCTGTT
GTCGGTGTGGTAGTCACTCATGGAACAGATTCCCTCGAAGAAACGGCCATTGCTGTAGATACTTTCCACAGCGACCCTCG
CCCCGTGGTGTTCACCGGAGCACAAAAACCATTTAACCATCCAGAATCTGATGGCCCTGGAAATCTGTTTGAAGCAATCA
TGATCGCATCTGATACCTCAGCACGAGGCATCGGCGCCTTGATTGTGTTCGGCCACGCAGTGATCCCAGCTCGTGGCTGC
ATTAAATGGCACACTTCAGACGATTTAGCATTCGCAACTAACGGCCCAGAAGAACCGCGGCGCGCCGACCCCATTGCGCC
TATTGCACTATGCGATGTTGTAGTCGATATCATCCCCGCCTATCCCGGCGCACCTCGACGGCTTATCGACGCCGCACTCG
CTGCCGGCGCCCAAGGTCTTGTCATTGAGGCGATGGGGTCTGGCAATGTCGGAACGGACATTGGGGTTGCGCTTGGTGAG
GCACTCGATGCGGGGATCCCCGTCGTCATATCTACGCGAGTCCCCCGAGGGGAAGTATTTGGTGCTTACGGAGGTGCTGG
TGGCGGCAAGACACTTGCATCCAAAGGGGCTGTCAACTCAACATATTTCCGATCCGGCCAAGCTCGTGTTTTGTTGGCAA
TCGCCATTGCCAGTGGTGTGCATCCCGCTACGTTGTTGTAG

Upstream 100 bases:

>100_bases
CCGTCGAAAGAGCCATTCTCACATACGGCTCCCCTTATTGCTACATTTCTCACCGGAAAATATGGCAGTGTTGTAAATCA
TGAGCAGCGGAACTGAATCA

Downstream 100 bases:

>100_bases
TTAAGACCAATCTAGGCTGTTGATAGGATCTGCGCTGCTCAAAAGGGCGTCGTTAGTGTCAAACGTACGCACTTTGCCAG
GTCCTGTGGTGCGGGTTTCA

Product: putative L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPKVIVLTTGGTLSCTADPNGALIPTVSGDELVAPIRQRFAGALDIEVCELHRLDSSSMTLRDIDDIVTATHNALEDDSV
VGVVVTHGTDSLEETAIAVDTFHSDPRPVVFTGAQKPFNHPESDGPGNLFEAIMIASDTSARGIGALIVFGHAVIPARGC
IKWHTSDDLAFATNGPEEPRRADPIAPIALCDVVVDIIPAYPGAPRRLIDAALAAGAQGLVIEAMGSGNVGTDIGVALGE
ALDAGIPVVISTRVPRGEVFGAYGGAGGGKTLASKGAVNSTYFRSGQARVLLAIAIASGVHPATLL

Sequences:

>Translated_306_residues
MPKVIVLTTGGTLSCTADPNGALIPTVSGDELVAPIRQRFAGALDIEVCELHRLDSSSMTLRDIDDIVTATHNALEDDSV
VGVVVTHGTDSLEETAIAVDTFHSDPRPVVFTGAQKPFNHPESDGPGNLFEAIMIASDTSARGIGALIVFGHAVIPARGC
IKWHTSDDLAFATNGPEEPRRADPIAPIALCDVVVDIIPAYPGAPRRLIDAALAAGAQGLVIEAMGSGNVGTDIGVALGE
ALDAGIPVVISTRVPRGEVFGAYGGAGGGKTLASKGAVNSTYFRSGQARVLLAIAIASGVHPATLL
>Mature_305_residues
PKVIVLTTGGTLSCTADPNGALIPTVSGDELVAPIRQRFAGALDIEVCELHRLDSSSMTLRDIDDIVTATHNALEDDSVV
GVVVTHGTDSLEETAIAVDTFHSDPRPVVFTGAQKPFNHPESDGPGNLFEAIMIASDTSARGIGALIVFGHAVIPARGCI
KWHTSDDLAFATNGPEEPRRADPIAPIALCDVVVDIIPAYPGAPRRLIDAALAAGAQGLVIEAMGSGNVGTDIGVALGEA
LDAGIPVVISTRVPRGEVFGAYGGAGGGKTLASKGAVNSTYFRSGQARVLLAIAIASGVHPATLL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=247, Percent_Identity=29.9595141700405, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788065, Length=246, Percent_Identity=28.0487804878049, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323189, Length=332, Percent_Identity=25.3012048192771, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323187, Length=332, Percent_Identity=25.3012048192771, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323186, Length=332, Percent_Identity=25.3012048192771, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323184, Length=332, Percent_Identity=25.3012048192771, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320527, Length=324, Percent_Identity=24.3827160493827, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24645530, Length=298, Percent_Identity=28.5234899328859, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 31352; Mature: 31221

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVIVLTTGGTLSCTADPNGALIPTVSGDELVAPIRQRFAGALDIEVCELHRLDSSSMT
CCEEEEEECCCEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
LRDIDDIVTATHNALEDDSVVGVVVTHGTDSLEETAIAVDTFHSDPRPVVFTGAQKPFNH
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHEEEEEECCCCCCCEEEECCCCCCCC
PESDGPGNLFEAIMIASDTSARGIGALIVFGHAVIPARGCIKWHTSDDLAFATNGPEEPR
CCCCCCCHHEEEEEEECCCCCCCCEEEEEECCEEECCCCEEEEECCCCEEEECCCCCCCC
RADPIAPIALCDVVVDIIPAYPGAPRRLIDAALAAGAQGLVIEAMGSGNVGTDIGVALGE
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
ALDAGIPVVISTRVPRGEVFGAYGGAGGGKTLASKGAVNSTYFRSGQARVLLAIAIASGV
HHCCCCCEEEEECCCCCCEEECCCCCCCCCHHHCCCCCCCCEEECCCCEEEEEEEECCCC
HPATLL
CCHHCC
>Mature Secondary Structure 
PKVIVLTTGGTLSCTADPNGALIPTVSGDELVAPIRQRFAGALDIEVCELHRLDSSSMT
CEEEEEECCCEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
LRDIDDIVTATHNALEDDSVVGVVVTHGTDSLEETAIAVDTFHSDPRPVVFTGAQKPFNH
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHEEEEEECCCCCCCEEEECCCCCCCC
PESDGPGNLFEAIMIASDTSARGIGALIVFGHAVIPARGCIKWHTSDDLAFATNGPEEPR
CCCCCCCHHEEEEEEECCCCCCCCEEEEEECCEEECCCCEEEEECCCCEEEECCCCCCCC
RADPIAPIALCDVVVDIIPAYPGAPRRLIDAALAAGAQGLVIEAMGSGNVGTDIGVALGE
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
ALDAGIPVVISTRVPRGEVFGAYGGAGGGKTLASKGAVNSTYFRSGQARVLLAIAIASGV
HHCCCCCEEEEECCCCCCEEECCCCCCCCCHHHCCCCCCCCEEECCCCEEEEEEEECCCC
HPATLL
CCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]