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The map label for this gene is hisF

Identifier: 38234133

GI number: 38234133

Start: 1586159

End: 1586935

Strand: Reverse

Name: hisF

Synonym: DIP1558

Alternate gene names: 38234133

Gene position: 1586935-1586159 (Counterclockwise)

Preceding gene: 38234134

Following gene: 38234132

Centisome position: 63.77

GC content: 52.12

Gene sequence:

>777_bases
ATGCCAGTCGCAGTTCGAGTCATTCCATGTTTGGACGTGAAAAACGGTCGCGTGGTTAAAGGAGTTAACTTTGAAGGGCT
TAAAGATGCCGGCGATCCAGTCGAGTTGGCTGCTCGTTATGATGCCGAGGGTGCCGACGAACTAACATTTCTCGATGTTT
CAGCATCTCAAGATGGCAGAGGAACCATGCTTGAAGTGGTGCGGCGAACAGCAGACCAAGTGTTTATTCCGTTAACTGTT
GGCGGTGGTGTTCGCAGCGCCGAGGACGTAGACCAACTACTGCGTGCAGGTGCTGACAAAGTCAGTGTGAATACTTCTGC
GATCGCTCGTCCTGAGTTACTTCAAGAGCTTTCTCAGCGTTTCGGTGCTCAGTGTGTCGTCTTAAGCGTGGATGCTCGCC
GAGTCCCAGAAGGAGGCAAGCCGCAGCCGTCGGGCTTTGAAGTAACCACACACGGCGGAACTCGTTCCGCTGGAATCGAT
GCTGTTGAGTGGGCGCAGAAAGGCGAGGCGCTGGGCGTGGGCGAAATCTTGTTGAACTCTATGGATGGCGATGGAACAAA
GCGTGGATTTGATCTGGAACTGATTGAAAAAGTCCGCCATGCGGTATCTATCCCCGTGATTGCTTCAGGTGGTGCAGGTA
AACCTGAGGACTTCCCCCCAGCAATAGCTAGTGGTGCTGATGCAGTTCTTGCAGCTTCAATTTTCCATTTCGGTGAAGTC
ACAATTTCTGAAGTGAAAAAGCAGGTAGTGGCTGCTGGCTACGAAGTTCGAAGCTAG

Upstream 100 bases:

>100_bases
AATGAGATAGCTATCTCGCGTTACTTTTCCGGCTGTTGTTTGACAAAAGTTACGCAGTTTTAAGTCCCTATGAATTTGTT
TCACACAAGGAGCTAACACC

Downstream 100 bases:

>100_bases
TACATAAGGAAGGAATATCACATGGACGATCCAGCATCCTATGAGCTCGATCCCAGCATAGCGCAGCGAGTCAAATTCAA
CAGTGCTGGGTTAGTGCCTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPVAVRVIPCLDVKNGRVVKGVNFEGLKDAGDPVELAARYDAEGADELTFLDVSASQDGRGTMLEVVRRTADQVFIPLTV
GGGVRSAEDVDQLLRAGADKVSVNTSAIARPELLQELSQRFGAQCVVLSVDARRVPEGGKPQPSGFEVTTHGGTRSAGID
AVEWAQKGEALGVGEILLNSMDGDGTKRGFDLELIEKVRHAVSIPVIASGGAGKPEDFPPAIASGADAVLAASIFHFGEV
TISEVKKQVVAAGYEVRS

Sequences:

>Translated_258_residues
MPVAVRVIPCLDVKNGRVVKGVNFEGLKDAGDPVELAARYDAEGADELTFLDVSASQDGRGTMLEVVRRTADQVFIPLTV
GGGVRSAEDVDQLLRAGADKVSVNTSAIARPELLQELSQRFGAQCVVLSVDARRVPEGGKPQPSGFEVTTHGGTRSAGID
AVEWAQKGEALGVGEILLNSMDGDGTKRGFDLELIEKVRHAVSIPVIASGGAGKPEDFPPAIASGADAVLAASIFHFGEV
TISEVKKQVVAAGYEVRS
>Mature_257_residues
PVAVRVIPCLDVKNGRVVKGVNFEGLKDAGDPVELAARYDAEGADELTFLDVSASQDGRGTMLEVVRRTADQVFIPLTVG
GGVRSAEDVDQLLRAGADKVSVNTSAIARPELLQELSQRFGAQCVVLSVDARRVPEGGKPQPSGFEVTTHGGTRSAGIDA
VEWAQKGEALGVGEILLNSMDGDGTKRGFDLELIEKVRHAVSIPVIASGGAGKPEDFPPAIASGADAVLAASIFHFGEVT
ISEVKKQVVAAGYEVRS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=43.8461538461538, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=29.0983606557377, Blast_Score=95, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=40, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CORDI (P60714)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939900.1
- ProteinModelPortal:   P60714
- SMR:   P60714
- GeneID:   2650747
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1558
- NMPDR:   fig|257309.1.peg.1491
- HOGENOM:   HBG541613
- OMA:   ARWEIFT
- PhylomeDB:   P60714
- ProtClustDB:   PRK02083
- BioCyc:   CDIP257309:DIP1558-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26990; Mature: 26859

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAVRVIPCLDVKNGRVVKGVNFEGLKDAGDPVELAARYDAEGADELTFLDVSASQDGR
CCEEEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHEECCCCCCCCEEEEEECCCCCCC
GTMLEVVRRTADQVFIPLTVGGGVRSAEDVDQLLRAGADKVSVNTSAIARPELLQELSQR
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHH
FGAQCVVLSVDARRVPEGGKPQPSGFEVTTHGGTRSAGIDAVEWAQKGEALGVGEILLNS
HCCEEEEEEECHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHCC
MDGDGTKRGFDLELIEKVRHAVSIPVIASGGAGKPEDFPPAIASGADAVLAASIFHFGEV
CCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCH
TISEVKKQVVAAGYEVRS
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVAVRVIPCLDVKNGRVVKGVNFEGLKDAGDPVELAARYDAEGADELTFLDVSASQDGR
CEEEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHEECCCCCCCCEEEEEECCCCCCC
GTMLEVVRRTADQVFIPLTVGGGVRSAEDVDQLLRAGADKVSVNTSAIARPELLQELSQR
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHH
FGAQCVVLSVDARRVPEGGKPQPSGFEVTTHGGTRSAGIDAVEWAQKGEALGVGEILLNS
HCCEEEEEEECHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHCC
MDGDGTKRGFDLELIEKVRHAVSIPVIASGGAGKPEDFPPAIASGADAVLAASIFHFGEV
CCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCH
TISEVKKQVVAAGYEVRS
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910