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The map label for this gene is ada [H]

Identifier: 38234068

GI number: 38234068

Start: 1508539

End: 1509048

Strand: Reverse

Name: ada [H]

Synonym: DIP1487

Alternate gene names: 38234068

Gene position: 1509048-1508539 (Counterclockwise)

Preceding gene: 161598673

Following gene: 38234067

Centisome position: 60.64

GC content: 49.61

Gene sequence:

>510_bases
GTGCACGCTGAGTACGGTTTTGTTTCCACTCCTGATGGCCAGTTTTGTGTTGTCACCGATTCGATGACACACAAAATTTT
GGCATCAGGGTGGACGGAAAATGTGAGCGAACTTGTTGGATTGATCCATAGAGACCTTCGACCGCTGAGTATGCGAGAGG
CTGAGGTTTCGCTATCTATACAAAATGTCATCAGTGCGTACTATGACGGCCGGTTCGCACGGATTCTCACAGTGCCGCTT
TTGCAACAGGCTACCGATTTTCGGATGTCTGTATGGTCTGCACTTCGAAGAATTCCGAGTGGTTCGCCAGTATCGTATGC
AACGTTGGCGCGGATGAGCGGACATGAAGGTGCGGTTAGAGCTGCGGCGTCGGCATGCGCCAACAATCCGGTGGCATTAT
TTGTGCCTTGTCATCGTGTAATTCGTTCTGATGGTTCTTACGGTGGATTTCGCTATGGTCTTGCCGTTAAGCGTTCGTTG
TTAACGCGAGAAGTTGCACATAATAAGTGA

Upstream 100 bases:

>100_bases
CCCAAGGTTGAAGGATTCGTATCTGGCAATGTTCATGCTAGTTATCTTCATGTCCATCCTGCTGCTGTTCCCGCCGCGGT
GAGAAGGTTTGTCGAGGCCT

Downstream 100 bases:

>100_bases
AAACGGTATCCATTAAGGAATGTCGCATTCAACTCCCGTCACCGTCTTGTCTGGTTTCCTAGGGTCAGGAAAAACCACGC
TTCTCAACCAAATGCTGTCC

Product: putative DNA repair-like protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MHAEYGFVSTPDGQFCVVTDSMTHKILASGWTENVSELVGLIHRDLRPLSMREAEVSLSIQNVISAYYDGRFARILTVPL
LQQATDFRMSVWSALRRIPSGSPVSYATLARMSGHEGAVRAAASACANNPVALFVPCHRVIRSDGSYGGFRYGLAVKRSL
LTREVAHNK

Sequences:

>Translated_169_residues
MHAEYGFVSTPDGQFCVVTDSMTHKILASGWTENVSELVGLIHRDLRPLSMREAEVSLSIQNVISAYYDGRFARILTVPL
LQQATDFRMSVWSALRRIPSGSPVSYATLARMSGHEGAVRAAASACANNPVALFVPCHRVIRSDGSYGGFRYGLAVKRSL
LTREVAHNK
>Mature_169_residues
MHAEYGFVSTPDGQFCVVTDSMTHKILASGWTENVSELVGLIHRDLRPLSMREAEVSLSIQNVISAYYDGRFARILTVPL
LQQATDFRMSVWSALRRIPSGSPVSYATLARMSGHEGAVRAAASACANNPVALFVPCHRVIRSDGSYGGFRYGLAVKRSL
LTREVAHNK

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=86, Percent_Identity=40.6976744186046, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788542, Length=94, Percent_Identity=50, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787596, Length=159, Percent_Identity=32.7044025157233, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=53.0120481927711, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=53.0120481927711, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=35.8024691358025, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18541; Mature: 18541

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAEYGFVSTPDGQFCVVTDSMTHKILASGWTENVSELVGLIHRDLRPLSMREAEVSLSI
CCCCCCCEECCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
QNVISAYYDGRFARILTVPLLQQATDFRMSVWSALRRIPSGSPVSYATLARMSGHEGAVR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHH
AAASACANNPVALFVPCHRVIRSDGSYGGFRYGLAVKRSLLTREVAHNK
HHHHHHCCCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHAEYGFVSTPDGQFCVVTDSMTHKILASGWTENVSELVGLIHRDLRPLSMREAEVSLSI
CCCCCCCEECCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
QNVISAYYDGRFARILTVPLLQQATDFRMSVWSALRRIPSGSPVSYATLARMSGHEGAVR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHH
AAASACANNPVALFVPCHRVIRSDGSYGGFRYGLAVKRSLLTREVAHNK
HHHHHHCCCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]