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The map label for this gene is nusA [H]

Identifier: 38234060

GI number: 38234060

Start: 1500079

End: 1501077

Strand: Reverse

Name: nusA [H]

Synonym: DIP1479

Alternate gene names: 38234060

Gene position: 1501077-1500079 (Counterclockwise)

Preceding gene: 38234061

Following gene: 38234059

Centisome position: 60.32

GC content: 47.85

Gene sequence:

>999_bases
GTGAATATTGACGTCCAAGCGCTGAAAGCAATTGAAGCGGATAGAAACATCGCTGTTGATGAATTGCTTGAAACAATTGC
ACGGGCTCTTCTGTTTGCGTACCAAGAGTACAAAGACACAAATACGGTTGAAAATTCCCGTGCGCGCGTGGACATCAATT
CGGTGACCGGCCACGTTTCGGTCATCGTCTCCGAGCTTGATGAAGACGGTGTAGTCACCACTGAATACGATGACACCCCT
GAAAATTTTGGACGCGTGGGAGCCCAAGCAGTTCGCGATGCCATTGTTCGTCGTTTGCGTGAAGCGGAAACACTGAAAGC
ATATGATGCCTACAGTGAATACGAAGGTCGCGTTGTGTCAGGTATCGTACAAGCTGATATTTTCGCCAACGAAAAAGGTA
TCGTAGTTATCCACCTTGGAACCGAGGTTGACGGCCAAGACGGAATTTTAATCCCTGCAGAACAAATTCCTGGTGAAAGC
TTTAAACACGGTGATCGCGTTAAAGCCTACGTTGTAGGAATTAACCGCACCCCACGTGATCTTCAGATCAATCTTTCACG
TACTCACCCAGAATTAGTTCGACGATTGTTTGAACTAGAAGTACCAGAAGTTGCTGATGGATCTGTAGAAATCATCGGTA
TCGCTCGTGAAGCGGGACACCGTTCTAAGGTAGCAGTGAAGGCGACGGTCAAGGGACTAAACGCTAAAGGTGCTTGCATT
GGACCACGTGGGCAGCGCGTTAACAACATTATGAATGAGCTTGGCGGCGAAAAGATCGATATCATCGACTTCGACGATGA
TCCAGCGAAGTTTGTTGGTAATGCGCTTGCACCATCAAAAGTCGTCCATGTAGAAATCACTGATGCTGAAGCTCAGACCG
CTCAGGTAACTGTGCCTGACTACCAATTGTCATTGGCGATTGGGAAAGAAGGGCAAAATGCACGCCTAGCAGCTCGTCTG
ACCGGTTGGAAAATCGACATCCGTTCAGATGCAAGCTAA

Upstream 100 bases:

>100_bases
AAGCGGTGGTAGAAATTGAATTCGCTAAACCTGCGGAAGATGAGTTAGCTTTAACCGCACTCGAATTTGATCAAGCCCTT
GATAGGGGAGAGGAAAACAA

Downstream 100 bases:

>100_bases
TTGCTTGTAGTGCTTAAGCCGTATCAATACTTCCAAGGGGGGAAGATGTTGATACGGCTATTTTTTAACAGGTTATTGTT
CTGGGCCAACATGGCGTACA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNIDVQALKAIEADRNIAVDELLETIARALLFAYQEYKDTNTVENSRARVDINSVTGHVSVIVSELDEDGVVTTEYDDTP
ENFGRVGAQAVRDAIVRRLREAETLKAYDAYSEYEGRVVSGIVQADIFANEKGIVVIHLGTEVDGQDGILIPAEQIPGES
FKHGDRVKAYVVGINRTPRDLQINLSRTHPELVRRLFELEVPEVADGSVEIIGIAREAGHRSKVAVKATVKGLNAKGACI
GPRGQRVNNIMNELGGEKIDIIDFDDDPAKFVGNALAPSKVVHVEITDAEAQTAQVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIRSDAS

Sequences:

>Translated_332_residues
MNIDVQALKAIEADRNIAVDELLETIARALLFAYQEYKDTNTVENSRARVDINSVTGHVSVIVSELDEDGVVTTEYDDTP
ENFGRVGAQAVRDAIVRRLREAETLKAYDAYSEYEGRVVSGIVQADIFANEKGIVVIHLGTEVDGQDGILIPAEQIPGES
FKHGDRVKAYVVGINRTPRDLQINLSRTHPELVRRLFELEVPEVADGSVEIIGIAREAGHRSKVAVKATVKGLNAKGACI
GPRGQRVNNIMNELGGEKIDIIDFDDDPAKFVGNALAPSKVVHVEITDAEAQTAQVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIRSDAS
>Mature_332_residues
MNIDVQALKAIEADRNIAVDELLETIARALLFAYQEYKDTNTVENSRARVDINSVTGHVSVIVSELDEDGVVTTEYDDTP
ENFGRVGAQAVRDAIVRRLREAETLKAYDAYSEYEGRVVSGIVQADIFANEKGIVVIHLGTEVDGQDGILIPAEQIPGES
FKHGDRVKAYVVGINRTPRDLQINLSRTHPELVRRLFELEVPEVADGSVEIIGIAREAGHRSKVAVKATVKGLNAKGACI
GPRGQRVNNIMNELGGEKIDIIDFDDDPAKFVGNALAPSKVVHVEITDAEAQTAQVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIRSDAS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=248, Percent_Identity=36.6935483870968, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 36177; Mature: 36177

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDVQALKAIEADRNIAVDELLETIARALLFAYQEYKDTNTVENSRARVDINSVTGHVS
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCEEE
VIVSELDEDGVVTTEYDDTPENFGRVGAQAVRDAIVRRLREAETLKAYDAYSEYEGRVVS
EEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIVQADIFANEKGIVVIHLGTEVDGQDGILIPAEQIPGESFKHGDRVKAYVVGINRTPRD
HEEEHHEEECCCCEEEEEECCCCCCCCCEEECHHHCCCCCCCCCCCEEEEEEECCCCCCC
LQINLSRTHPELVRRLFELEVPEVADGSVEIIGIAREAGHRSKVAVKATVKGLNAKGACI
EEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCEE
GPRGQRVNNIMNELGGEKIDIIDFDDDPAKFVGNALAPSKVVHVEITDAEAQTAQVTVPD
CCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCC
YQLSLAIGKEGQNARLAARLTGWKIDIRSDAS
EEEEEEECCCCCCCEEEEEEECEEEEECCCCC
>Mature Secondary Structure
MNIDVQALKAIEADRNIAVDELLETIARALLFAYQEYKDTNTVENSRARVDINSVTGHVS
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCEEE
VIVSELDEDGVVTTEYDDTPENFGRVGAQAVRDAIVRRLREAETLKAYDAYSEYEGRVVS
EEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIVQADIFANEKGIVVIHLGTEVDGQDGILIPAEQIPGESFKHGDRVKAYVVGINRTPRD
HEEEHHEEECCCCEEEEEECCCCCCCCCEEECHHHCCCCCCCCCCCEEEEEEECCCCCCC
LQINLSRTHPELVRRLFELEVPEVADGSVEIIGIAREAGHRSKVAVKATVKGLNAKGACI
EEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCEE
GPRGQRVNNIMNELGGEKIDIIDFDDDPAKFVGNALAPSKVVHVEITDAEAQTAQVTVPD
CCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCC
YQLSLAIGKEGQNARLAARLTGWKIDIRSDAS
EEEEEEECCCCCCCEEEEEEECEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]