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The map label for this gene is entD [C]

Identifier: 38234053

GI number: 38234053

Start: 1492097

End: 1492789

Strand: Reverse

Name: entD [C]

Synonym: DIP1472

Alternate gene names: 38234053

Gene position: 1492789-1492097 (Counterclockwise)

Preceding gene: 38234054

Following gene: 38234051

Centisome position: 59.98

GC content: 47.76

Gene sequence:

>693_bases
ATGATAGCAGGTAATAATAGCGAGTATGCCCTGGATTCGAGGTTGTTCCCGCAATCAGCTCGTAGTACTGCATTATTGGT
TCCAAGGCATACACCGGATTTATCAAATTTTAATAGACTGCATGTTTTAGAAAAAGCACAGGTGAAAAATGCGGTTGCCG
TTCGCCGTGCTGAGTTTGGTGACGCACGTTGGTGTGCACACCAAAGTTTGCGCAAGTTGGGGCTATATGATCATCCGGCA
ATTTTACGCGGTGAACGGGGTATGCCTTTGTGGCCGGTTGGCATTGCTGGATCTTTAACGCATACGGAGGGTTTGAGGGC
GGCTGTTGTTGCACCGACTACTGAAGTAGCTTCTATGGGCATTGATGCAGAGATCGCTGAGGAGTTGCCTGGCGGTATTT
TGGGTTCAATTGCGCGGCCCAATGAAATCGCCATGTTGGATGATCTGCGTGCTAGAGGTTTATTATTCGCTGATCGGTTG
TTGTTTTGTGCCAAAGAAGCCACCTATAAGGCTTGGTTCCCGATTACTCAACGATGGCTGGATTTTGATCAGGCAGAAAT
TGATATTAGGGCCGATGGCACGTTTATTTCTTATTTGCTCATTCGGCCCACCCCGTTTCCTTTTATCGAAGGAAAGTGGG
CAATACATGATGGCTATGTCGTAGCAACCACTGTCATCCCCGCGATGGGTTAA

Upstream 100 bases:

>100_bases
TTCGTCACGTAGAAGTCTCGCTTGGCTACCCGCATCAATGGTCACAAAATATCGAAGATCGTTCGTGGCCGTTTCCCGTC
ATGACCTCGGAGGTGGTGGC

Downstream 100 bases:

>100_bases
AGGGTCGATGGACGTGCCACAAATATGGTGGCTAGCCGTTTGCCTTGTTCTTTTACCAAGGCGATAGCACGTCCATGAGG
ATCGACCGCTGCATGGATTC

Product: putative transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Phosphopantetheinyl Transferase; Phosphopantetheinyl Transferase PptA; Sfp-Type Phosphopantetheinyl Transferase; 4-Phosphopantetheinyl Tranferase; Siderophore Biosynthesis Protein; Holo-[Acyl-Carrier-Protein] Synthase; EntD Family Protein; Phosphopantetheinyl Transferase PptII; 4-Phosphopantetheinyl Transferase Family Protein; Transferase; 4-Phosphopantetheinyl Transferase EntD; 4-Phosphopantetheinyl Transferase Superfamily; Vibriobactin Synthase Component D; 4-Phosphopantetheinyl Transferase Protein

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIAGNNSEYALDSRLFPQSARSTALLVPRHTPDLSNFNRLHVLEKAQVKNAVAVRRAEFGDARWCAHQSLRKLGLYDHPA
ILRGERGMPLWPVGIAGSLTHTEGLRAAVVAPTTEVASMGIDAEIAEELPGGILGSIARPNEIAMLDDLRARGLLFADRL
LFCAKEATYKAWFPITQRWLDFDQAEIDIRADGTFISYLLIRPTPFPFIEGKWAIHDGYVVATTVIPAMG

Sequences:

>Translated_230_residues
MIAGNNSEYALDSRLFPQSARSTALLVPRHTPDLSNFNRLHVLEKAQVKNAVAVRRAEFGDARWCAHQSLRKLGLYDHPA
ILRGERGMPLWPVGIAGSLTHTEGLRAAVVAPTTEVASMGIDAEIAEELPGGILGSIARPNEIAMLDDLRARGLLFADRL
LFCAKEATYKAWFPITQRWLDFDQAEIDIRADGTFISYLLIRPTPFPFIEGKWAIHDGYVVATTVIPAMG
>Mature_230_residues
MIAGNNSEYALDSRLFPQSARSTALLVPRHTPDLSNFNRLHVLEKAQVKNAVAVRRAEFGDARWCAHQSLRKLGLYDHPA
ILRGERGMPLWPVGIAGSLTHTEGLRAAVVAPTTEVASMGIDAEIAEELPGGILGSIARPNEIAMLDDLRARGLLFADRL
LFCAKEATYKAWFPITQRWLDFDQAEIDIRADGTFISYLLIRPTPFPFIEGKWAIHDGYVVATTVIPAMG

Specific function: Catalyzes The Transfer Of The 4'-Phosphopantetheine Moiety From Coenzyme A To Apo-Domains Of Both Entb (An Arcp Domain) And Entf (A Pcp Domain). Plays An Essential Role In The Assembly Of The Enterobactin. [C]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.8.- [C]

Molecular weight: Translated: 25377; Mature: 25377

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGNNSEYALDSRLFPQSARSTALLVPRHTPDLSNFNRLHVLEKAQVKNAVAVRRAEFG
CCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCC
DARWCAHQSLRKLGLYDHPAILRGERGMPLWPVGIAGSLTHTEGLRAAVVAPTTEVASMG
CHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHCC
IDAEIAEELPGGILGSIARPNEIAMLDDLRARGLLFADRLLFCAKEATYKAWFPITQRWL
CCHHHHHHCCCCHHHCCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHCCCEEECCHHHHHC
DFDQAEIDIRADGTFISYLLIRPTPFPFIEGKWAIHDGYVVATTVIPAMG
CCCCCEEEEEECCEEEEEEEECCCCCCEECCEEEEECCEEEEEEECCCCC
>Mature Secondary Structure
MIAGNNSEYALDSRLFPQSARSTALLVPRHTPDLSNFNRLHVLEKAQVKNAVAVRRAEFG
CCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCC
DARWCAHQSLRKLGLYDHPAILRGERGMPLWPVGIAGSLTHTEGLRAAVVAPTTEVASMG
CHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHCC
IDAEIAEELPGGILGSIARPNEIAMLDDLRARGLLFADRLLFCAKEATYKAWFPITQRWL
CCHHHHHHCCCCHHHCCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHCCCEEECCHHHHHC
DFDQAEIDIRADGTFISYLLIRPTPFPFIEGKWAIHDGYVVATTVIPAMG
CCCCCEEEEEECCEEEEEEEECCCCCCEECCEEEEECCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA