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The map label for this gene is rihA [H]

Identifier: 38234050

GI number: 38234050

Start: 1489207

End: 1490166

Strand: Reverse

Name: rihA [H]

Synonym: DIP1469

Alternate gene names: 38234050

Gene position: 1490166-1489207 (Counterclockwise)

Preceding gene: 38234051

Following gene: 38234049

Centisome position: 59.88

GC content: 50.0

Gene sequence:

>960_bases
ATGAAAAAAATTATCCTTGATTTGGACACTGGTATCGATGATGCTCTGGCATTGGCCTATACGTTAGGTTCTCCTGAACT
TGATCTCATCGGTGTGACCGCCACTTATGGAAACGTCTTGGTAGAAACAGGCGTACGAAACGATCTTGCTCTTCTTGAAC
TTTTCGGGCGTAGCGACGTTCCTGTTTTTTCTGGTGAACCGCATGCGCTGACGAAAGATGGCTTTGAAGTTCTGGAAATC
TCTGCATTCATCCATGGCAAAAATGGGATTGGTGAGGCCGAGGTTGCGGAACCAGTTGGTGTTGTACAGGAACTCAGCGC
GGTCGATTTCTTGATTGAATCCGTTGAGCGTTACGGAGATGAACTCATCATTGTTCCCACTGGCGCAATGACCAACATAG
CTGCAGCTATGAAAAAGAGTGAGACTTTTGCAAGGGATGCACAGATCGTATTTATGGGTGGTGCGCTTACGGTTCCTGGC
AATGTCAGTCAGTGGGCAGAAGCCAATGTCAATCAAGACCCTGAGGCTGCGGATATCATGGTGCGCAATGCTGGAGATAT
TACGATGGTGGGCCTTGATGTGACTCTGCAAACGCTTCTCACGTATGCGGAAACCGCTACGTGGCGAGCGCTTGGTACAC
CTGCTGGCAATTTCTTGGCTGATGCGACGGACTATTACATCAAGGCATATGACACCACAGCCCCTCACCTTGGCGGCTGC
GGTTTGCACGATCCATTGGCTGTGGGTGTCGCGATCGATCCATCGCTGGTGACCTTGCTACCTATTAACCTCAAAGTGGA
CACTGAGGGGCCCACACGTGGACGTACCATTGGTGACGAAGTCCGCCTTAACGATCCACATAAGAACTGTAAGGTTGCTG
TTGGGGTAGACGTCGATCGATTCCTCAAGGAATTCATGGAACGAATTACTCGGGTTGCTCAAGGTAGCAATGCGCGATGA

Upstream 100 bases:

>100_bases
GATGAGTTGCTTGTTGCTATTGAAAATGATGTAACTCAAACGCGTGCCATATTGCATATCTAAATCCATCCCCTCCGAAC
GCAGCTAAAGGACCGTCGAG

Downstream 100 bases:

>100_bases
TGAAATAGATATTTAGAAAAATGATACGGCAGCGGCACACTATGTGCTAAGCTGCTCGAGGTTCTTTTACTGCGGTTCAC
AGCAGTGAGGTTCCGTGTTC

Product: putative nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKIILDLDTGIDDALALAYTLGSPELDLIGVTATYGNVLVETGVRNDLALLELFGRSDVPVFSGEPHALTKDGFEVLEI
SAFIHGKNGIGEAEVAEPVGVVQELSAVDFLIESVERYGDELIIVPTGAMTNIAAAMKKSETFARDAQIVFMGGALTVPG
NVSQWAEANVNQDPEAADIMVRNAGDITMVGLDVTLQTLLTYAETATWRALGTPAGNFLADATDYYIKAYDTTAPHLGGC
GLHDPLAVGVAIDPSLVTLLPINLKVDTEGPTRGRTIGDEVRLNDPHKNCKVAVGVDVDRFLKEFMERITRVAQGSNAR

Sequences:

>Translated_319_residues
MKKIILDLDTGIDDALALAYTLGSPELDLIGVTATYGNVLVETGVRNDLALLELFGRSDVPVFSGEPHALTKDGFEVLEI
SAFIHGKNGIGEAEVAEPVGVVQELSAVDFLIESVERYGDELIIVPTGAMTNIAAAMKKSETFARDAQIVFMGGALTVPG
NVSQWAEANVNQDPEAADIMVRNAGDITMVGLDVTLQTLLTYAETATWRALGTPAGNFLADATDYYIKAYDTTAPHLGGC
GLHDPLAVGVAIDPSLVTLLPINLKVDTEGPTRGRTIGDEVRLNDPHKNCKVAVGVDVDRFLKEFMERITRVAQGSNAR
>Mature_319_residues
MKKIILDLDTGIDDALALAYTLGSPELDLIGVTATYGNVLVETGVRNDLALLELFGRSDVPVFSGEPHALTKDGFEVLEI
SAFIHGKNGIGEAEVAEPVGVVQELSAVDFLIESVERYGDELIIVPTGAMTNIAAAMKKSETFARDAQIVFMGGALTVPG
NVSQWAEANVNQDPEAADIMVRNAGDITMVGLDVTLQTLLTYAETATWRALGTPAGNFLADATDYYIKAYDTTAPHLGGC
GLHDPLAVGVAIDPSLVTLLPINLKVDTEGPTRGRTIGDEVRLNDPHKNCKVAVGVDVDRFLKEFMERITRVAQGSNAR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=312, Percent_Identity=33.0128205128205, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1788486, Length=303, Percent_Identity=32.013201320132, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786213, Length=311, Percent_Identity=33.4405144694534, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17565698, Length=334, Percent_Identity=27.8443113772455, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI32565474, Length=292, Percent_Identity=28.4246575342466, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=334, Percent_Identity=23.0538922155689, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583915, Length=190, Percent_Identity=32.1052631578947, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34011; Mature: 34011

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIILDLDTGIDDALALAYTLGSPELDLIGVTATYGNVLVETGVRNDLALLELFGRSDV
CCEEEEEECCCCCHHHEEEEECCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHCCCCC
PVFSGEPHALTKDGFEVLEISAFIHGKNGIGEAEVAEPVGVVQELSAVDFLIESVERYGD
CEECCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELIIVPTGAMTNIAAAMKKSETFARDAQIVFMGGALTVPGNVSQWAEANVNQDPEAADIM
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHCCCCCCCCCCEEE
VRNAGDITMVGLDVTLQTLLTYAETATWRALGTPAGNFLADATDYYIKAYDTTAPHLGGC
EECCCCEEEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCEEEEEEECCCCCCCCCC
GLHDPLAVGVAIDPSLVTLLPINLKVDTEGPTRGRTIGDEVRLNDPHKNCKVAVGVDVDR
CCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEECCHHH
FLKEFMERITRVAQGSNAR
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKIILDLDTGIDDALALAYTLGSPELDLIGVTATYGNVLVETGVRNDLALLELFGRSDV
CCEEEEEECCCCCHHHEEEEECCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHCCCCC
PVFSGEPHALTKDGFEVLEISAFIHGKNGIGEAEVAEPVGVVQELSAVDFLIESVERYGD
CEECCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELIIVPTGAMTNIAAAMKKSETFARDAQIVFMGGALTVPGNVSQWAEANVNQDPEAADIM
EEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHCCCCCCCCCCEEE
VRNAGDITMVGLDVTLQTLLTYAETATWRALGTPAGNFLADATDYYIKAYDTTAPHLGGC
EECCCCEEEEEHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCEEEEEEECCCCCCCCCC
GLHDPLAVGVAIDPSLVTLLPINLKVDTEGPTRGRTIGDEVRLNDPHKNCKVAVGVDVDR
CCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEECCHHH
FLKEFMERITRVAQGSNAR
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA