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The map label for this gene is sigB

Identifier: 38233998

GI number: 38233998

Start: 1430121

End: 1431110

Strand: Direct

Name: sigB

Synonym: DIP1413

Alternate gene names: 38233998

Gene position: 1430121-1431110 (Clockwise)

Preceding gene: 38233997

Following gene: 38233999

Centisome position: 57.47

GC content: 50.0

Gene sequence:

>990_bases
ATGACAAGCCCTTCCGACGTGGAAGCATCCACGGAGACCGTCGACCGCGGTAGCCGCAGGAATCAAACGAATGATAACCC
TTCAGCAGATCTCGTTCGTGTCTACCTCAATGGCATCGGCAAAACTGCCCTACTCAATGCTGAAGATGAGGTCGAGCTCG
CACAGACAATTGAAGTCGGACTCTATGCGGAATATCTCTTAGAAAATTCCCAAGAGCCACTGACCCGCGCAATGAAAAGG
GACCTTAAAGTATTGGTTAAGGAAGGGCGCAAGGCACGTTCTCACCTACTAGAGGCGAATCTACGTCTTGTGGTATCCTT
AGCAAAGCGTTACACCGGACGCGGCATGCCCCTACTGGACCTCATTCAAGAAGGTAATTTGGGACTCATCCGCGCAATGG
AAAAGTTTGATTACTCCAAGGGATTTAAGTTTTCCACCTATGCCACTTGGTGGATCCGCCAAGCCATCACACGCGGCATG
GCAGATCAGTCCCGAACAATTCGCTTACCCGTCCACCTCGTAGAGCAGGTAAACAAGCTTTCTCGAATCAAACGCGAGAT
GTATCAACAGCTCGGACGTGAAGCAACCAACGAAGAGCTTGCGGAAGAATCTGGCATTGAAGAATCCAAAATTGAAATGT
TGCTTCGCCAGTCTCGTGACCCAGTAAGCCTAGATATGCCAGTTGGTGCCGATGAAGAGGCTCCACTTGGTGACTTTATC
GAAGATTCCGAGGCGACTGATGCCGAATCAGCTGTAGTTGCGTCGTTGCGTCACTCTGATATCAGAACAGTCTTGGACAC
TTTGGAGCAACGTGAACAGGACGTTATTCGCCTTCGTTATGGGCTCGATGATGGAGTTCCCCGCACTTTAGATCAAATTG
GTCGTCAATTTGGACTGTCTCGTGAAAGAGTTCGCCAAATTGAACGCGAGGTCATGTCGAAACTGCGTGACGGTGCCCGT
GCCGAAAAACTTCGAGCATACGCACAATAA

Upstream 100 bases:

>100_bases
TTGTCGAATGCCCATAGTTTCCCCACACATGGGGGTAATGGAACTAGATTTATCGATTCGCCGTTGTTCCATGTGTAGCA
ACGAGAGCTAGGAGGTCATT

Downstream 100 bases:

>100_bases
AGCGTTTGCTTAGATATGCCTACCAATAAAGACATAAACGCCTATTAAAAGCAATCTTTAGATTAGGCGTTATAATTAAA
GTCTCATCGAAAAGCGCGCT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTSPSDVEASTETVDRGSRRNQTNDNPSADLVRVYLNGIGKTALLNAEDEVELAQTIEVGLYAEYLLENSQEPLTRAMKR
DLKVLVKEGRKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGM
ADQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDFI
EDSEATDAESAVVASLRHSDIRTVLDTLEQREQDVIRLRYGLDDGVPRTLDQIGRQFGLSRERVRQIEREVMSKLRDGAR
AEKLRAYAQ

Sequences:

>Translated_329_residues
MTSPSDVEASTETVDRGSRRNQTNDNPSADLVRVYLNGIGKTALLNAEDEVELAQTIEVGLYAEYLLENSQEPLTRAMKR
DLKVLVKEGRKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGM
ADQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDFI
EDSEATDAESAVVASLRHSDIRTVLDTLEQREQDVIRLRYGLDDGVPRTLDQIGRQFGLSRERVRQIEREVMSKLRDGAR
AEKLRAYAQ
>Mature_328_residues
TSPSDVEASTETVDRGSRRNQTNDNPSADLVRVYLNGIGKTALLNAEDEVELAQTIEVGLYAEYLLENSQEPLTRAMKRD
LKVLVKEGRKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMA
DQSRTIRLPVHLVEQVNKLSRIKREMYQQLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDFIE
DSEATDAESAVVASLRHSDIRTVLDTLEQREQDVIRLRYGLDDGVPRTLDQIGRQFGLSRERVRQIEREVMSKLRDGARA
EKLRAYAQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=52.2821576763486, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=39.9305555555556, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=30.672268907563, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPSB_CORDI (P33118)

Other databases:

- EMBL:   BX248358
- EMBL:   M80338
- EMBL:   M34239
- RefSeq:   NP_939765.1
- ProteinModelPortal:   P33118
- SMR:   P33118
- GeneID:   2649488
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1413
- NMPDR:   fig|257309.1.peg.1356
- HOGENOM:   HBG745096
- OMA:   LEAPARE
- ProtClustDB:   PRK07921
- BioCyc:   CDIP257309:DIP1413-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 37351; Mature: 37220

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPSDVEASTETVDRGSRRNQTNDNPSADLVRVYLNGIGKTALLNAEDEVELAQTIEVG
CCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHH
LYAEYLLENSQEPLTRAMKRDLKVLVKEGRKARSHLLEANLRLVVSLAKRYTGRGMPLLD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKL
HHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHH
SRIKREMYQQLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDFI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHH
EDSEATDAESAVVASLRHSDIRTVLDTLEQREQDVIRLRYGLDDGVPRTLDQIGRQFGLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
RERVRQIEREVMSKLRDGARAEKLRAYAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TSPSDVEASTETVDRGSRRNQTNDNPSADLVRVYLNGIGKTALLNAEDEVELAQTIEVG
CCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHH
LYAEYLLENSQEPLTRAMKRDLKVLVKEGRKARSHLLEANLRLVVSLAKRYTGRGMPLLD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKL
HHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHH
SRIKREMYQQLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDFI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHH
EDSEATDAESAVVASLRHSDIRTVLDTLEQREQDVIRLRYGLDDGVPRTLDQIGRQFGLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
RERVRQIEREVMSKLRDGARAEKLRAYAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013