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The map label for this gene is hisS

Identifier: 38233946

GI number: 38233946

Start: 1378566

End: 1379837

Strand: Reverse

Name: hisS

Synonym: DIP1360

Alternate gene names: 38233946

Gene position: 1379837-1378566 (Counterclockwise)

Preceding gene: 38233947

Following gene: 38233943

Centisome position: 55.45

GC content: 51.97

Gene sequence:

>1272_bases
GTGAGTAAAAAGAAGCTGCAGACGATCCAAGCCCCTAAAGGCGTGCCAGACTATATTCCACCAGTTTCCCCCGAATTTTT
AGGGGTGCGTGATACTTTTGCCCACCAAGCGCATCTGGCTGGTTATGAGCACATCGAGCTGCCGATCTTTGAAGACACCA
CTTTGTTTGCCCGTGGCGTCGGTGAATCGACCGATGTGGTAACGAAGGAAATGTACACGTTTGCGGATCGAGGGGATCGG
TCGGTTACGCTTCGTCCTGAAGGCACAGCCGGTGTGATGCGTGCAGTTATCGAGCACAATCTCGATCGCGGACAACTTCC
GGTCAAGCTCAACTACTATGGTCCGTTTTTCCGCTATGAGCGCCCGCAAGCGGGTCGTTACCGCCAGCTGCAACAAGTAG
GCGTTGAAGCTATCGGAGTTGATGACCCCGCTCTCGACGCGGAAGTTATTGCACTTGCGGACCGCTCTCTCAAGGTAATT
GGCTTGACCGGTTACCGTTTGGAGCTTACGAGCCTTGGCGACCATACGTGCCGTCCGCAATACCGAGAAAAGCTCCAAGA
ATTCTTGTTTAAGCTGCCTTTGGACGAAGAAACTCGTCATCGTGCGGAGATTAATCCACTGCGAGTTTTGGACGATAAGC
GTCCAGAAATTCAAGAGATGACCGTAGATGCGCCGTTGATGTTGGATAACCTGTCTGATTCGAGTAGGGAACACTTCGAG
CTTGTTACGGGAATGCTGGATGACCTCAATGTTGCCTATGTAGTCAATCCTCGAATGGTTCGCGGTTTGGACTACTACAC
CAAGACCACATTTGAGTTTGTTCATGACGGTTTGGGTGCACAGTCGGGTATTGGAGGCGGTGGGCGTTACGACGGCCTCA
TGGCGCAGTTGGGCGGCCAAGAACTTTCAGGTATCGGCTATGCACTGGGAGTGGACCGTTGCTTGCTTGCGCTTGAAGCG
GAACAAAAGCGAGTAACCGATGGCTCTCGAGTGAACGTTTTTGGTGTGGCTATGGGTTCGGCAGCGAAGCGCGAAATGGT
TCGGATCATCGACGCACTTCGTTCAGCTGGTATCAGCGCAGATATGGCCTATGGTGATCGCGGTTTGAAAGGTGCGATGA
AAGGTGCGGACCGCGCTGGAGCTCGTTTTGCTCTTGTGTTAGGGGATCGTGAGCTTGAAAACAATTGTGTTGCTGTTAAA
GATCTAGCTCAACAAGGGCAACGCGATGTATCCCTTGACAATTTGATCTTGGAGATTCAGTCAGCGTTGTAA

Upstream 100 bases:

>100_bases
CATGGTCCAGCTACCACGATGCGAGCTGAGAAGGCTTCAAATCCTTATTTGAGGGTGTAGCACTCGAATTGTTTTGTTGC
TACCGCTAAGCTGTATCGAC

Downstream 100 bases:

>100_bases
GACACGAGGAGGCCACGCTTGCGGCGGTGGGAATGCACAGTATTTTTATTACTCGTGCATTCCCACCGCTTTTAGCATTC
AGTGATGCTGACAGGAAGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSKKKLQTIQAPKGVPDYIPPVSPEFLGVRDTFAHQAHLAGYEHIELPIFEDTTLFARGVGESTDVVTKEMYTFADRGDR
SVTLRPEGTAGVMRAVIEHNLDRGQLPVKLNYYGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIALADRSLKVI
GLTGYRLELTSLGDHTCRPQYREKLQEFLFKLPLDEETRHRAEINPLRVLDDKRPEIQEMTVDAPLMLDNLSDSSREHFE
LVTGMLDDLNVAYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQELSGIGYALGVDRCLLALEA
EQKRVTDGSRVNVFGVAMGSAAKREMVRIIDALRSAGISADMAYGDRGLKGAMKGADRAGARFALVLGDRELENNCVAVK
DLAQQGQRDVSLDNLILEIQSAL

Sequences:

>Translated_423_residues
MSKKKLQTIQAPKGVPDYIPPVSPEFLGVRDTFAHQAHLAGYEHIELPIFEDTTLFARGVGESTDVVTKEMYTFADRGDR
SVTLRPEGTAGVMRAVIEHNLDRGQLPVKLNYYGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIALADRSLKVI
GLTGYRLELTSLGDHTCRPQYREKLQEFLFKLPLDEETRHRAEINPLRVLDDKRPEIQEMTVDAPLMLDNLSDSSREHFE
LVTGMLDDLNVAYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQELSGIGYALGVDRCLLALEA
EQKRVTDGSRVNVFGVAMGSAAKREMVRIIDALRSAGISADMAYGDRGLKGAMKGADRAGARFALVLGDRELENNCVAVK
DLAQQGQRDVSLDNLILEIQSAL
>Mature_422_residues
SKKKLQTIQAPKGVPDYIPPVSPEFLGVRDTFAHQAHLAGYEHIELPIFEDTTLFARGVGESTDVVTKEMYTFADRGDRS
VTLRPEGTAGVMRAVIEHNLDRGQLPVKLNYYGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIALADRSLKVIG
LTGYRLELTSLGDHTCRPQYREKLQEFLFKLPLDEETRHRAEINPLRVLDDKRPEIQEMTVDAPLMLDNLSDSSREHFEL
VTGMLDDLNVAYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQELSGIGYALGVDRCLLALEAE
QKRVTDGSRVNVFGVAMGSAAKREMVRIIDALRSAGISADMAYGDRGLKGAMKGADRAGARFALVLGDRELENNCVAVKD
LAQQGQRDVSLDNLILEIQSAL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=474, Percent_Identity=23.6286919831224, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI15029520, Length=472, Percent_Identity=24.1525423728814, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=42.4170616113744, Blast_Score=324, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI71993693, Length=469, Percent_Identity=24.3070362473348, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71993686, Length=469, Percent_Identity=24.3070362473348, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CORDI (P60914)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939713.1
- ProteinModelPortal:   P60914
- SMR:   P60914
- GeneID:   2649891
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1360
- NMPDR:   fig|257309.1.peg.1304
- HOGENOM:   HBG616575
- OMA:   RYETNIL
- PhylomeDB:   P60914
- ProtClustDB:   PRK00037
- BioCyc:   CDIP257309:DIP1360-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46692; Mature: 46561

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKKLQTIQAPKGVPDYIPPVSPEFLGVRDTFAHQAHLAGYEHIELPIFEDTTLFARGV
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHCCCC
GESTDVVTKEMYTFADRGDRSVTLRPEGTAGVMRAVIEHNLDRGQLPVKLNYYGPFFRYE
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
RPQAGRYRQLQQVGVEAIGVDDPALDAEVIALADRSLKVIGLTGYRLELTSLGDHTCRPQ
CCCCHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCHH
YREKLQEFLFKLPLDEETRHRAEINPLRVLDDKRPEIQEMTVDAPLMLDNLSDSSREHFE
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCHHHEECCCCEEECCCCCCHHHHHH
LVTGMLDDLNVAYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQ
HHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
ELSGIGYALGVDRCLLALEAEQKRVTDGSRVNVFGVAMGSAAKREMVRIIDALRSAGISA
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
DMAYGDRGLKGAMKGADRAGARFALVLGDRELENNCVAVKDLAQQGQRDVSLDNLILEIQ
EECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SAL
HCC
>Mature Secondary Structure 
SKKKLQTIQAPKGVPDYIPPVSPEFLGVRDTFAHQAHLAGYEHIELPIFEDTTLFARGV
CCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHCCCC
GESTDVVTKEMYTFADRGDRSVTLRPEGTAGVMRAVIEHNLDRGQLPVKLNYYGPFFRYE
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCC
RPQAGRYRQLQQVGVEAIGVDDPALDAEVIALADRSLKVIGLTGYRLELTSLGDHTCRPQ
CCCCHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCHH
YREKLQEFLFKLPLDEETRHRAEINPLRVLDDKRPEIQEMTVDAPLMLDNLSDSSREHFE
HHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCHHHEECCCCEEECCCCCCHHHHHH
LVTGMLDDLNVAYVVNPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQ
HHHHHHHCCCEEEEECCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
ELSGIGYALGVDRCLLALEAEQKRVTDGSRVNVFGVAMGSAAKREMVRIIDALRSAGISA
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
DMAYGDRGLKGAMKGADRAGARFALVLGDRELENNCVAVKDLAQQGQRDVSLDNLILEIQ
EECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910