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The map label for this gene is cysS

Identifier: 38233849

GI number: 38233849

Start: 1267559

End: 1268803

Strand: Reverse

Name: cysS

Synonym: DIP1261

Alternate gene names: 38233849

Gene position: 1268803-1267559 (Counterclockwise)

Preceding gene: 38233850

Following gene: 38233848

Centisome position: 50.98

GC content: 47.79

Gene sequence:

>1245_bases
ATGCAATCTTGGCCCATGCCGACAATTCCTTCAGTGCCGGGTAAACCGGCTGTACTGTCCTTGTTTGATACCAGTGATCA
ACTCATTAAACCTGTTGAAGTGGACACTCCAGAAGTTGGGGTTTATGTGTGTGGAATTACCCCTTATGACTCCACACATT
TAGGACATGCAGCGACATATTTGACGTTTGATCTAATCAATAGGCAGCTTATCGACGCAGGCCACCGTGTTCATTTCGTT
CAAAACATCACTGACGTCGATGAGCCTCTTTTTGAACGTGCATTGAGAGATGGCGTTGACTGGCGAGAGTTAGGCTCCAG
CCAAATCGATCTTTTTAGATCAGACATGGAGAACCTTAGCGTTATTCCTCCTCGGCACTATGTTGGTGCAATGGAGTCTA
TTCCAGAAGTAATTGCAATGGTGGAAACCATGCTTGCCAATGGCACGGCGTACATCGTTGAATCAGACTCCTACCGTGAT
ATTTATGCATCCATAACTGCGACTACCCAGTTTGGATACGAGTCCCATTACTCTCGTGAACTAATGGAACAATTTTTTGC
CGAACGTGGTGGAGACCCCGAACGTGTCGGGAAAAAAGATTCGCTTGATGCTCTTATATGGAGAGCACATCGACTAGGTG
AACCTTCTTGGGATGCGCCATTCGGGGCGGGGCGCCCTGGATGGCACGTTGAGTGTTCCGCGATCGCAACACATCATTTG
GGAAGCCATTTCGCAATTCAAGGAGGCGGATCAGATCTTATCTTCCCACACCACGAATTCTCGGCTGCTCACGCTGAGGC
AACATTTGGCGAGTCCCGAATGGCAGGTCACTATGTGCACACAGGAATGATCGGACTTGACGGCACAAAAATGAGCAAGT
CCTTAGGCAATTTAGTATTCGTATCGCGACTGACCTCAGAAGGGTATCATCCATCTGACATTAGACTTGGAGTTTTTGCA
GGTCATTATCGCGACGATCGTGACTGGTCAACTGACCTCCTCACAGCTGCACAGGAGCGCAGACAAGCGTGGATTACTGC
AGCTGATAATGCTCGAGACGTACAACAAGTTCAACAAACAATTCAAAAAATACGTCATTTATTGGCTAACGACTTAAATA
CCCCTGCAGTCCTCGCCGAAGTGGACAACTGGGCCAACTTGATCCCGCTAAATGATTCAGCTCGTACTGAGGCTGGAAGG
CTCATGGCATCTGGTTTGGATGCATTATTGGGAGTGAAGATATAA

Upstream 100 bases:

>100_bases
CCCTGTCGGTTTGCTTGTTATGGCATTACTAGCTACCGGAATGCTAACCGCTTAACTACTGTAATGTGTCATTGCTGCCT
CAGCAGGTAATGTTGATGGC

Downstream 100 bases:

>100_bases
ATGACCGATATGTCCGACTCTTTTTCATTTATTGACGACTTCCTGCGCGATCTTGAAGAGTTTGGTACCGGCTCCTACCT
TCGTGCCGATGAAAAGGAAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MQSWPMPTIPSVPGKPAVLSLFDTSDQLIKPVEVDTPEVGVYVCGITPYDSTHLGHAATYLTFDLINRQLIDAGHRVHFV
QNITDVDEPLFERALRDGVDWRELGSSQIDLFRSDMENLSVIPPRHYVGAMESIPEVIAMVETMLANGTAYIVESDSYRD
IYASITATTQFGYESHYSRELMEQFFAERGGDPERVGKKDSLDALIWRAHRLGEPSWDAPFGAGRPGWHVECSAIATHHL
GSHFAIQGGGSDLIFPHHEFSAAHAEATFGESRMAGHYVHTGMIGLDGTKMSKSLGNLVFVSRLTSEGYHPSDIRLGVFA
GHYRDDRDWSTDLLTAAQERRQAWITAADNARDVQQVQQTIQKIRHLLANDLNTPAVLAEVDNWANLIPLNDSARTEAGR
LMASGLDALLGVKI

Sequences:

>Translated_414_residues
MQSWPMPTIPSVPGKPAVLSLFDTSDQLIKPVEVDTPEVGVYVCGITPYDSTHLGHAATYLTFDLINRQLIDAGHRVHFV
QNITDVDEPLFERALRDGVDWRELGSSQIDLFRSDMENLSVIPPRHYVGAMESIPEVIAMVETMLANGTAYIVESDSYRD
IYASITATTQFGYESHYSRELMEQFFAERGGDPERVGKKDSLDALIWRAHRLGEPSWDAPFGAGRPGWHVECSAIATHHL
GSHFAIQGGGSDLIFPHHEFSAAHAEATFGESRMAGHYVHTGMIGLDGTKMSKSLGNLVFVSRLTSEGYHPSDIRLGVFA
GHYRDDRDWSTDLLTAAQERRQAWITAADNARDVQQVQQTIQKIRHLLANDLNTPAVLAEVDNWANLIPLNDSARTEAGR
LMASGLDALLGVKI
>Mature_414_residues
MQSWPMPTIPSVPGKPAVLSLFDTSDQLIKPVEVDTPEVGVYVCGITPYDSTHLGHAATYLTFDLINRQLIDAGHRVHFV
QNITDVDEPLFERALRDGVDWRELGSSQIDLFRSDMENLSVIPPRHYVGAMESIPEVIAMVETMLANGTAYIVESDSYRD
IYASITATTQFGYESHYSRELMEQFFAERGGDPERVGKKDSLDALIWRAHRLGEPSWDAPFGAGRPGWHVECSAIATHHL
GSHFAIQGGGSDLIFPHHEFSAAHAEATFGESRMAGHYVHTGMIGLDGTKMSKSLGNLVFVSRLTSEGYHPSDIRLGVFA
GHYRDDRDWSTDLLTAAQERRQAWITAADNARDVQQVQQTIQKIRHLLANDLNTPAVLAEVDNWANLIPLNDSARTEAGR
LMASGLDALLGVKI

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=397, Percent_Identity=29.7229219143577, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI10835051, Length=249, Percent_Identity=32.9317269076305, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21269877, Length=249, Percent_Identity=32.9317269076305, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI62240992, Length=249, Percent_Identity=32.9317269076305, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI303304987, Length=249, Percent_Identity=32.9317269076305, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1786737, Length=405, Percent_Identity=33.0864197530864, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17509797, Length=257, Percent_Identity=31.1284046692607, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17509795, Length=257, Percent_Identity=31.1284046692607, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324082, Length=269, Percent_Identity=31.5985130111524, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922218, Length=320, Percent_Identity=35.3125, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19922364, Length=244, Percent_Identity=33.1967213114754, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_CORDI (Q6NH81)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939616.1
- ProteinModelPortal:   Q6NH81
- SMR:   Q6NH81
- GeneID:   2649815
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1261
- NMPDR:   fig|257309.1.peg.1207
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- BioCyc:   CDIP257309:DIP1261-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.3.1.13

Molecular weight: Translated: 45805; Mature: 45805

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 228-228 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWPMPTIPSVPGKPAVLSLFDTSDQLIKPVEVDTPEVGVYVCGITPYDSTHLGHAATY
CCCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
LTFDLINRQLIDAGHRVHFVQNITDVDEPLFERALRDGVDWRELGSSQIDLFRSDMENLS
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCC
VIPPRHYVGAMESIPEVIAMVETMLANGTAYIVESDSYRDIYASITATTQFGYESHYSRE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEHHHCCCHHHHHHH
LMEQFFAERGGDPERVGKKDSLDALIWRAHRLGEPSWDAPFGAGRPGWHVECSAIATHHL
HHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHC
GSHFAIQGGGSDLIFPHHEFSAAHAEATFGESRMAGHYVHTGMIGLDGTKMSKSLGNLVF
CCEEEEECCCCCEECCCCCCCHHHHHHHCCCHHHCCHHHEECEEECCCHHHHHHHCCEEE
VSRLTSEGYHPSDIRLGVFAGHYRDDRDWSTDLLTAAQERRQAWITAADNARDVQQVQQT
EEHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHH
IQKIRHLLANDLNTPAVLAEVDNWANLIPLNDSARTEAGRLMASGLDALLGVKI
HHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSWPMPTIPSVPGKPAVLSLFDTSDQLIKPVEVDTPEVGVYVCGITPYDSTHLGHAATY
CCCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
LTFDLINRQLIDAGHRVHFVQNITDVDEPLFERALRDGVDWRELGSSQIDLFRSDMENLS
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCC
VIPPRHYVGAMESIPEVIAMVETMLANGTAYIVESDSYRDIYASITATTQFGYESHYSRE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEHHHCCCHHHHHHH
LMEQFFAERGGDPERVGKKDSLDALIWRAHRLGEPSWDAPFGAGRPGWHVECSAIATHHL
HHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHC
GSHFAIQGGGSDLIFPHHEFSAAHAEATFGESRMAGHYVHTGMIGLDGTKMSKSLGNLVF
CCEEEEECCCCCEECCCCCCCHHHHHHHCCCHHHCCHHHEECEEECCCHHHHHHHCCEEE
VSRLTSEGYHPSDIRLGVFAGHYRDDRDWSTDLLTAAQERRQAWITAADNARDVQQVQQT
EEHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHH
IQKIRHLLANDLNTPAVLAEVDNWANLIPLNDSARTEAGRLMASGLDALLGVKI
HHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910