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The map label for this gene is trmI [H]

Identifier: 38233837

GI number: 38233837

Start: 1252639

End: 1253475

Strand: Reverse

Name: trmI [H]

Synonym: DIP1249

Alternate gene names: 38233837

Gene position: 1253475-1252639 (Counterclockwise)

Preceding gene: 38233838

Following gene: 38233836

Centisome position: 50.37

GC content: 51.37

Gene sequence:

>837_bases
ATGGCAAACTCTGGACCATTTCAGCCTGGTGATCGTGTGCAACTCACAGATCCCAAGCGCCGGCATGCAACGATAGTTCT
TGAAACAGGCGGTGCGTATCACACGCATAAAGGCATGATTCATCATGACGATATTATCGGAAACGCTGAAGGCACTGTCG
TTAAGTCTGAGCAAGGATACGAATACTTATGTTTCAGACACCTGATGGTCGATCACGTGCTTTCTATGCCTCGCGGTGCT
GCAGTGATCTATCCAAAAGATTCTGCCCAAATTTTGGTCGAAGGAGATATTTTCCCCGGCGCGCGGGTGTTAGAAGCCGG
TGCAGGCTCAGGTGCTCTATCGATGGCGCTATTGCGGGCTGTTGGCCACGAAGGTCATGTCTTTTCTTATGAGATTCGAG
AAGACCACCTTGAATATGCCGAAAATAACGTTGATGAATTTTTCGGCTACCGTCCCGAAACATGGTCCCCTCGCTTAGGT
GATTTGCAGGATGTAACTGTTGAAGATCTGGGTGGTCCAGTAGACCGCATCATCTTGGACATGCTGGCCCCTTGGGAGTG
TTTGGAGACAGCGTCTCGATTGCTCATCCCTGGCGGAGTGCTAATGACGTATGTAGCGACCGTCCCTCAGCTGATGAAGG
TCATGGAGGGGATTCGCGAACTTAAATGTTTTACCGAGCCTCGTGCTTGGGAATCTCTCGTCCGTGAATGGCGTGTAGAA
GGGTTGGCTACGCGGCCTGAACACCGTATGAATGCTCACACTGCATTCTTGATTTGGACACGTCGACTTGCCGACGGTAC
CGTTCCACCACGTCCACAGCGTCGCGCACGTAAGTAG

Upstream 100 bases:

>100_bases
TCCGTTGCTTTCCATGCACTCTGCTCGAGAATTAGCCAGCACCACAGATATCTATTGGTTATCACGGTGTCTGCACGCGT
ATCTGGTAGGTTAAATTCTC

Downstream 100 bases:

>100_bases
ACTCCTAGCAGGTTTTTAAGTGGCACCTTTAAAAAGGTGCCACTTATTGCAGTCTAATGGCAACACCAGTGTTGGGAAAT
TAGTAAGGTAAGGGTCATGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MANSGPFQPGDRVQLTDPKRRHATIVLETGGAYHTHKGMIHHDDIIGNAEGTVVKSEQGYEYLCFRHLMVDHVLSMPRGA
AVIYPKDSAQILVEGDIFPGARVLEAGAGSGALSMALLRAVGHEGHVFSYEIREDHLEYAENNVDEFFGYRPETWSPRLG
DLQDVTVEDLGGPVDRIILDMLAPWECLETASRLLIPGGVLMTYVATVPQLMKVMEGIRELKCFTEPRAWESLVREWRVE
GLATRPEHRMNAHTAFLIWTRRLADGTVPPRPQRRARK

Sequences:

>Translated_278_residues
MANSGPFQPGDRVQLTDPKRRHATIVLETGGAYHTHKGMIHHDDIIGNAEGTVVKSEQGYEYLCFRHLMVDHVLSMPRGA
AVIYPKDSAQILVEGDIFPGARVLEAGAGSGALSMALLRAVGHEGHVFSYEIREDHLEYAENNVDEFFGYRPETWSPRLG
DLQDVTVEDLGGPVDRIILDMLAPWECLETASRLLIPGGVLMTYVATVPQLMKVMEGIRELKCFTEPRAWESLVREWRVE
GLATRPEHRMNAHTAFLIWTRRLADGTVPPRPQRRARK
>Mature_277_residues
ANSGPFQPGDRVQLTDPKRRHATIVLETGGAYHTHKGMIHHDDIIGNAEGTVVKSEQGYEYLCFRHLMVDHVLSMPRGAA
VIYPKDSAQILVEGDIFPGARVLEAGAGSGALSMALLRAVGHEGHVFSYEIREDHLEYAENNVDEFFGYRPETWSPRLGD
LQDVTVEDLGGPVDRIILDMLAPWECLETASRLLIPGGVLMTYVATVPQLMKVMEGIRELKCFTEPRAWESLVREWRVEG
LATRPEHRMNAHTAFLIWTRRLADGTVPPRPQRRARK

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=246, Percent_Identity=31.7073170731707, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI123173772, Length=244, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17509459, Length=222, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322336, Length=181, Percent_Identity=28.1767955801105, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 31202; Mature: 31071

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSGPFQPGDRVQLTDPKRRHATIVLETGGAYHTHKGMIHHDDIIGNAEGTVVKSEQGY
CCCCCCCCCCCEEEECCCCCCEEEEEEECCCCEECCCCCEECCCCCCCCCCCEEECCCCC
EYLCFRHLMVDHVLSMPRGAAVIYPKDSAQILVEGDIFPGARVLEAGAGSGALSMALLRA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCHHHEECCCCCHHHHHHHHHH
VGHEGHVFSYEIREDHLEYAENNVDEFFGYRPETWSPRLGDLQDVTVEDLGGPVDRIILD
HCCCCCEEEEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
MLAPWECLETASRLLIPGGVLMTYVATVPQLMKVMEGIRELKCFTEPRAWESLVREWRVE
HHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLATRPEHRMNAHTAFLIWTRRLADGTVPPRPQRRARK
CCCCCCCHHHCCCEEEEEEEHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
ANSGPFQPGDRVQLTDPKRRHATIVLETGGAYHTHKGMIHHDDIIGNAEGTVVKSEQGY
CCCCCCCCCCEEEECCCCCCEEEEEEECCCCEECCCCCEECCCCCCCCCCCEEECCCCC
EYLCFRHLMVDHVLSMPRGAAVIYPKDSAQILVEGDIFPGARVLEAGAGSGALSMALLRA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCHHHEECCCCCHHHHHHHHHH
VGHEGHVFSYEIREDHLEYAENNVDEFFGYRPETWSPRLGDLQDVTVEDLGGPVDRIILD
HCCCCCEEEEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
MLAPWECLETASRLLIPGGVLMTYVATVPQLMKVMEGIRELKCFTEPRAWESLVREWRVE
HHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLATRPEHRMNAHTAFLIWTRRLADGTVPPRPQRRARK
CCCCCCCHHHCCCEEEEEEEHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA