Click here to switch to the map view.

The map label for this gene is bioB2

Identifier: 38233714

GI number: 38233714

Start: 1109532

End: 1110524

Strand: Reverse

Name: bioB2

Synonym: DIP1124

Alternate gene names: 38233714

Gene position: 1110524-1109532 (Counterclockwise)

Preceding gene: 38233716

Following gene: 38233713

Centisome position: 44.62

GC content: 57.4

Gene sequence:

>993_bases
ATGACTGCCACATTGAACACAGTTCAGGATCTCGAAGCATCAGTACTTGCCGGCACCGCCATCACGCGAGACGAGGCCCT
CTCGCTTATCGACGCACCCCTTGACGAACTCAGCGCAGCAGCCGACCGCATCCGCGCCCAGATGTGTGGCGACGGATTCG
ACATGTGCAGCATCATCAACGCCAAATCCGGTCGATGCCCAGAAAACTGCACCTTCTGCGCGCAAAGCATCAGGTACCCC
ACCATCAGCGTGGACTCCTACCCGCTGATCACCGCAGACGAACTCGTACGACAAGCACAAGAAAACAAAGACAAAGGCGT
AATCCGATTTTCCATCGTCACCTCCGGCCGCAAGCTACGCCGCGACGAGGTCCGCCACATTTGCGAGGGCGTACGCCGAA
TCAAACAAGAAGTCGGCATCGAAGTATGTATCTCCGCAGGTTTACTAAGCGCCGAAGATTTCCAAGCTCTCCACGATGCC
GGTATTTCTCGCGTGCACTGCAACCTAGAAACCTCCCGCGCATACTTCCCGAGCATCTGCATCTCACACACCTTTGACGA
TAAAATCGCAACCCTACAAGCAGCACGCGACGAAGGCATGAGCCTATGCTCCGGCGGAATCCTAGGGCTTGGCGAGTCCA
TGGAAGACCGCATCGACATGGCACTATCCGCCCGTGAGCTCGGGGTCAACTCATTCCCCGTCAACGTACTCGTGGCCATC
GAAGGCACCCCGCTGGCTGGAACCGAACAATTACGCCCCGAAGAAGTACAACGCTGCGTAGCCATCTTTCGTTTTATCCT
CCCCCAAGCGGCAATTAGGCTCGCCGGCGGCCGCGAACTACTTGGCGACGACGGCAAGGCCTGCTTCCAATCCGGCGCAA
ACTCCGCCATCAGCGGCGACATGCTCACCACCACCGGCACCACCATCGCCAGTGACATGGCACTAGTCAAAGATCTCGGA
TACACCGTCACCTTGGACCACTCTCACAGTTAA

Upstream 100 bases:

>100_bases
GCGAGTCGCGGGCTGATCATGAGATCATCCTAAGCTAACGCGTCGAACACCACAGTCGTTACAGCAGATCTTTGCGATTG
AAAGGTGTTCAATAGGGACT

Downstream 100 bases:

>100_bases
GAAATAGCAACTTTGGCACGGTGATGTTTTAGGATGTTTCCTCGTGACTGATACCCAAGCCCAAGGTCTAGACACCTTGC
CACCGTGCCCAGAGTGCGAA

Product: biotin synthase family transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTATLNTVQDLEASVLAGTAITRDEALSLIDAPLDELSAAADRIRAQMCGDGFDMCSIINAKSGRCPENCTFCAQSIRYP
TISVDSYPLITADELVRQAQENKDKGVIRFSIVTSGRKLRRDEVRHICEGVRRIKQEVGIEVCISAGLLSAEDFQALHDA
GISRVHCNLETSRAYFPSICISHTFDDKIATLQAARDEGMSLCSGGILGLGESMEDRIDMALSARELGVNSFPVNVLVAI
EGTPLAGTEQLRPEEVQRCVAIFRFILPQAAIRLAGGRELLGDDGKACFQSGANSAISGDMLTTTGTTIASDMALVKDLG
YTVTLDHSHS

Sequences:

>Translated_330_residues
MTATLNTVQDLEASVLAGTAITRDEALSLIDAPLDELSAAADRIRAQMCGDGFDMCSIINAKSGRCPENCTFCAQSIRYP
TISVDSYPLITADELVRQAQENKDKGVIRFSIVTSGRKLRRDEVRHICEGVRRIKQEVGIEVCISAGLLSAEDFQALHDA
GISRVHCNLETSRAYFPSICISHTFDDKIATLQAARDEGMSLCSGGILGLGESMEDRIDMALSARELGVNSFPVNVLVAI
EGTPLAGTEQLRPEEVQRCVAIFRFILPQAAIRLAGGRELLGDDGKACFQSGANSAISGDMLTTTGTTIASDMALVKDLG
YTVTLDHSHS
>Mature_329_residues
TATLNTVQDLEASVLAGTAITRDEALSLIDAPLDELSAAADRIRAQMCGDGFDMCSIINAKSGRCPENCTFCAQSIRYPT
ISVDSYPLITADELVRQAQENKDKGVIRFSIVTSGRKLRRDEVRHICEGVRRIKQEVGIEVCISAGLLSAEDFQALHDAG
ISRVHCNLETSRAYFPSICISHTFDDKIATLQAARDEGMSLCSGGILGLGESMEDRIDMALSARELGVNSFPVNVLVAIE
GTPLAGTEQLRPEEVQRCVAIFRFILPQAAIRLAGGRELLGDDGKACFQSGANSAISGDMLTTTGTTIASDMALVKDLGY
TVTLDHSHS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=31.4935064935065, Blast_Score=168, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=32.8025477707006, Blast_Score=182, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB2_CORDI (Q6NHL3)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939481.1
- HSSP:   P12996
- ProteinModelPortal:   Q6NHL3
- SMR:   Q6NHL3
- GeneID:   2649926
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1124
- NMPDR:   fig|257309.1.peg.1072
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- PhylomeDB:   Q6NHL3
- ProtClustDB:   CLSK2760945
- BioCyc:   CDIP257309:DIP1124-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35448; Mature: 35316

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLNTVQDLEASVLAGTAITRDEALSLIDAPLDELSAAADRIRAQMCGDGFDMCSIIN
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHC
AKSGRCPENCTFCAQSIRYPTISVDSYPLITADELVRQAQENKDKGVIRFSIVTSGRKLR
CCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHH
RDEVRHICEGVRRIKQEVGIEVCISAGLLSAEDFQALHDAGISRVHCNLETSRAYFPSIC
HHHHHHHHHHHHHHHHHCCHHEEHHCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHCHHH
ISHTFDDKIATLQAARDEGMSLCSGGILGLGESMEDRIDMALSARELGVNSFPVNVLVAI
HHCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
EGTPLAGTEQLRPEEVQRCVAIFRFILPQAAIRLAGGRELLGDDGKACFQSGANSAISGD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCCCCCCC
MLTTTGTTIASDMALVKDLGYTVTLDHSHS
EEECCCCHHHHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure 
TATLNTVQDLEASVLAGTAITRDEALSLIDAPLDELSAAADRIRAQMCGDGFDMCSIIN
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHC
AKSGRCPENCTFCAQSIRYPTISVDSYPLITADELVRQAQENKDKGVIRFSIVTSGRKLR
CCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHH
RDEVRHICEGVRRIKQEVGIEVCISAGLLSAEDFQALHDAGISRVHCNLETSRAYFPSIC
HHHHHHHHHHHHHHHHHCCHHEEHHCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHCHHH
ISHTFDDKIATLQAARDEGMSLCSGGILGLGESMEDRIDMALSARELGVNSFPVNVLVAI
HHCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
EGTPLAGTEQLRPEEVQRCVAIFRFILPQAAIRLAGGRELLGDDGKACFQSGANSAISGD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCCCCCCC
MLTTTGTTIASDMALVKDLGYTVTLDHSHS
EEECCCCHHHHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910