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The map label for this gene is alkK [H]

Identifier: 38233633

GI number: 38233633

Start: 1019270

End: 1021021

Strand: Reverse

Name: alkK [H]

Synonym: DIP1038

Alternate gene names: 38233633

Gene position: 1021021-1019270 (Counterclockwise)

Preceding gene: 38233651

Following gene: 38233627

Centisome position: 41.03

GC content: 52.8

Gene sequence:

>1752_bases
ATGGTGATTATCATGCTCAGCACAATGCAGGACATTCCGCTTAACCTCGCGCGCATTCTCACCTACGGTGCCACCGCCCA
CGCGGACACAAAGATCACCACCTTTGATGGCGAGGTAGCATCAGAGACGACATATGCGGAAATTGCCGCACGAGCTGCGG
CTTTCGCCCACGCGCTTCACGACGAACTGGGTATTGACGATGACCAGCGAGTCGGATCGATGATGTATAACTGCGCAGAA
CATCTCGAAGTTCTTTTTGCGGTTTCTTGCATGGGTGCTGTATTTAATCCGCTGAATAAGCAATTAATGAATGATCAGAT
TCGCCACATCATTAATCATGCTGAAGATGAAGTCATCGTTGCTGATCAACGCCTCGCTAAACAACTGGGCACGATCCTGC
ATCAAGGATGCCCTAGTGTGCGTGCCGTGATCTTTATCGGAACAAGCCCTATCACGGATGCAGCCACGGAAATACCCGAG
GGAATTACCTGCTACTCCTACGAATCGCTTCTCGACGGTCGCAGCACCGTCTACGAGTGGCCACAAGTTGAAGAAACCAC
AGCAGCGGCGATCTGCTACTCCACCGGCACCACTGGAGCACCCAAAGGGGTGGCATATTCCCACCGTGCGTTGTATCTCC
AAGCGCTAAATCTTCGCACCACAGACTCGCTCGCCGTTACCCACGGGCAGTCATTTTTGTGCTGCGTGCCGATCTATCAC
ATCTTGAGCTGGTGTGTCCCCATCGCAGCCTTCATGTCCGGCACTCCCCTCGTATTCCCTGGGTCTAGTGTGTCGGCTCC
TTCCCTTGCACACATCATCGCTACTGCGCATCCTCGTGTTGCTCACGGTGTTCCCACGTTATGGATCCAGCTCATGGTCC
ATTACATGCGTCACTCTCCTGAACGTATGAGCCTGCAAGAAATCTATGTGGGAGGTTCCGCTGTACCGGCGATCTTGATC
AAGCTTTGGGAAGAACGCTACGGCGTCGATGTAGTCCACGTGTGGGGCATGACTGAAACGGTTGCAATCGGTACGGTGGC
ACGACCACCATCGGGAGTTTCAGGTGAAACCCGACTGAACTATCGCATTAGTCAAGGTCGGTTCCCCGCCACTTTGGAAT
ATCGAGTAGTCAATGATGGCGAAATCATGAGCTCGACCGACCGTAACCAAGGTGAAATTCAAGTCCGAGGAAATTGGGTC
ACCGAACACTACTACCACTCAGCGACCGAGGACAATGGCGGTGCCTCGTCGATGTTCCGCGACCACGAAGTAGAGGATGC
ACCTGAACAGTTCACTGAGGATGGTTGGCTTCGCACAGGTGATGTTGGTTCAGTAACTCGCGACGGTTACCTCACCATCC
ATGACCGCGCTCGCGATGTCATCCGCTCAGGCGGCGAATGGATCTACTCCACAATGTTGGAAAACGAAATCATGGCAGCA
ACAGTCGTCGTTGAGGCAGCAGTAATTGGATATCCCGATCCTAAATGGGGCGAACGACCGCTGTCAGTGACAGTCTTAGC
TGCAGGTGTGGAACCGAATAAAGAAACAGCTGAACGCCTACGCGAGGGGTTACGCGATACGTTCCCCAACTGGATGCTTC
CGGAATACTGGACGTTTGTTAAATCCATTGACAAAACATCCGTGGGCAAGTTTGACAAAATTGACCTGCGCCAGCACTTG
GCTGATGGCGATTTTGAAATCATTGCACTTAAAGGCCCAGGTAGATCCTCCCATAGCGATTCCGCCGAATGA

Upstream 100 bases:

>100_bases
AACATCGAACCGTGGGCAAGCACAGAAAGTTTCCGTGAAGTTCGTAGCGATTCTAACCCATAAAGTAGGTTTAAGTGAAC
TTTGTGCCGAAAACACAACT

Downstream 100 bases:

>100_bases
CGACTACCGCCCTCCTAGAGACTTGACTAGGAGGGCGGTAGTTAAATTGGCATGCGGTTATCTAGGCATCGACTTTCACC
GATACAGCATCTGCTACATC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MVIIMLSTMQDIPLNLARILTYGATAHADTKITTFDGEVASETTYAEIAARAAAFAHALHDELGIDDDQRVGSMMYNCAE
HLEVLFAVSCMGAVFNPLNKQLMNDQIRHIINHAEDEVIVADQRLAKQLGTILHQGCPSVRAVIFIGTSPITDAATEIPE
GITCYSYESLLDGRSTVYEWPQVEETTAAAICYSTGTTGAPKGVAYSHRALYLQALNLRTTDSLAVTHGQSFLCCVPIYH
ILSWCVPIAAFMSGTPLVFPGSSVSAPSLAHIIATAHPRVAHGVPTLWIQLMVHYMRHSPERMSLQEIYVGGSAVPAILI
KLWEERYGVDVVHVWGMTETVAIGTVARPPSGVSGETRLNYRISQGRFPATLEYRVVNDGEIMSSTDRNQGEIQVRGNWV
TEHYYHSATEDNGGASSMFRDHEVEDAPEQFTEDGWLRTGDVGSVTRDGYLTIHDRARDVIRSGGEWIYSTMLENEIMAA
TVVVEAAVIGYPDPKWGERPLSVTVLAAGVEPNKETAERLREGLRDTFPNWMLPEYWTFVKSIDKTSVGKFDKIDLRQHL
ADGDFEIIALKGPGRSSHSDSAE

Sequences:

>Translated_583_residues
MVIIMLSTMQDIPLNLARILTYGATAHADTKITTFDGEVASETTYAEIAARAAAFAHALHDELGIDDDQRVGSMMYNCAE
HLEVLFAVSCMGAVFNPLNKQLMNDQIRHIINHAEDEVIVADQRLAKQLGTILHQGCPSVRAVIFIGTSPITDAATEIPE
GITCYSYESLLDGRSTVYEWPQVEETTAAAICYSTGTTGAPKGVAYSHRALYLQALNLRTTDSLAVTHGQSFLCCVPIYH
ILSWCVPIAAFMSGTPLVFPGSSVSAPSLAHIIATAHPRVAHGVPTLWIQLMVHYMRHSPERMSLQEIYVGGSAVPAILI
KLWEERYGVDVVHVWGMTETVAIGTVARPPSGVSGETRLNYRISQGRFPATLEYRVVNDGEIMSSTDRNQGEIQVRGNWV
TEHYYHSATEDNGGASSMFRDHEVEDAPEQFTEDGWLRTGDVGSVTRDGYLTIHDRARDVIRSGGEWIYSTMLENEIMAA
TVVVEAAVIGYPDPKWGERPLSVTVLAAGVEPNKETAERLREGLRDTFPNWMLPEYWTFVKSIDKTSVGKFDKIDLRQHL
ADGDFEIIALKGPGRSSHSDSAE
>Mature_583_residues
MVIIMLSTMQDIPLNLARILTYGATAHADTKITTFDGEVASETTYAEIAARAAAFAHALHDELGIDDDQRVGSMMYNCAE
HLEVLFAVSCMGAVFNPLNKQLMNDQIRHIINHAEDEVIVADQRLAKQLGTILHQGCPSVRAVIFIGTSPITDAATEIPE
GITCYSYESLLDGRSTVYEWPQVEETTAAAICYSTGTTGAPKGVAYSHRALYLQALNLRTTDSLAVTHGQSFLCCVPIYH
ILSWCVPIAAFMSGTPLVFPGSSVSAPSLAHIIATAHPRVAHGVPTLWIQLMVHYMRHSPERMSLQEIYVGGSAVPAILI
KLWEERYGVDVVHVWGMTETVAIGTVARPPSGVSGETRLNYRISQGRFPATLEYRVVNDGEIMSSTDRNQGEIQVRGNWV
TEHYYHSATEDNGGASSMFRDHEVEDAPEQFTEDGWLRTGDVGSVTRDGYLTIHDRARDVIRSGGEWIYSTMLENEIMAA
TVVVEAAVIGYPDPKWGERPLSVTVLAAGVEPNKETAERLREGLRDTFPNWMLPEYWTFVKSIDKTSVGKFDKIDLRQHL
ADGDFEIIALKGPGRSSHSDSAE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=550, Percent_Identity=25.0909090909091, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI187761343, Length=550, Percent_Identity=25.0909090909091, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=22.011385199241, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI156151445, Length=539, Percent_Identity=21.8923933209648, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI42544132, Length=527, Percent_Identity=21.2523719165085, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=565, Percent_Identity=23.1858407079646, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI221142682, Length=542, Percent_Identity=22.140221402214, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1786810, Length=573, Percent_Identity=25.3054101221641, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693145, Length=543, Percent_Identity=21.5469613259669, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790505, Length=601, Percent_Identity=23.4608985024958, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=528, Percent_Identity=26.8939393939394, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=531, Percent_Identity=23.5404896421846, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=553, Percent_Identity=23.869801084991, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=553, Percent_Identity=23.869801084991, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=553, Percent_Identity=23.869801084991, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=553, Percent_Identity=22.7848101265823, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=562, Percent_Identity=24.5551601423488, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=594, Percent_Identity=23.5690235690236, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=562, Percent_Identity=23.8434163701068, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541856, Length=512, Percent_Identity=21.2890625, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=336, Percent_Identity=23.8095238095238, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=487, Percent_Identity=20.9445585215606, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581924, Length=450, Percent_Identity=27.3333333333333, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24656500, Length=482, Percent_Identity=25.3112033195021, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=22.7871939736347, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922652, Length=526, Percent_Identity=23.574144486692, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64159; Mature: 64159

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIIMLSTMQDIPLNLARILTYGATAHADTKITTFDGEVASETTYAEIAARAAAFAHALH
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DELGIDDDQRVGSMMYNCAEHLEVLFAVSCMGAVFNPLNKQLMNDQIRHIINHAEDEVIV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ADQRLAKQLGTILHQGCPSVRAVIFIGTSPITDAATEIPEGITCYSYESLLDGRSTVYEW
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCEEEEHHHHHCCCHHEECC
PQVEETTAAAICYSTGTTGAPKGVAYSHRALYLQALNLRTTDSLAVTHGQSFLCCVPIYH
CCCCCCHHEEEEEECCCCCCCCCCCCCHHEEEEEEECCCCCCCEEEECCCCEEHHHHHHH
ILSWCVPIAAFMSGTPLVFPGSSVSAPSLAHIIATAHPRVAHGVPTLWIQLMVHYMRHSP
HHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCC
ERMSLQEIYVGGSAVPAILIKLWEERYGVDVVHVWGMTETVAIGTVARPPSGVSGETRLN
CCCCHHHHEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCEEEE
YRISQGRFPATLEYRVVNDGEIMSSTDRNQGEIQVRGNWVTEHYYHSATEDNGGASSMFR
EEECCCCCCCEEEEEEECCCCEECCCCCCCCEEEEECCEEEHHHCCCCCCCCCCHHHHHC
DHEVEDAPEQFTEDGWLRTGDVGSVTRDGYLTIHDRARDVIRSGGEWIYSTMLENEIMAA
CCCCCCCHHHHCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
TVVVEAAVIGYPDPKWGERPLSVTVLAAGVEPNKETAERLREGLRDTFPNWMLPEYWTFV
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
KSIDKTSVGKFDKIDLRQHLADGDFEIIALKGPGRSSHSDSAE
HHHCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MVIIMLSTMQDIPLNLARILTYGATAHADTKITTFDGEVASETTYAEIAARAAAFAHALH
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DELGIDDDQRVGSMMYNCAEHLEVLFAVSCMGAVFNPLNKQLMNDQIRHIINHAEDEVIV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ADQRLAKQLGTILHQGCPSVRAVIFIGTSPITDAATEIPEGITCYSYESLLDGRSTVYEW
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCEEEEHHHHHCCCHHEECC
PQVEETTAAAICYSTGTTGAPKGVAYSHRALYLQALNLRTTDSLAVTHGQSFLCCVPIYH
CCCCCCHHEEEEEECCCCCCCCCCCCCHHEEEEEEECCCCCCCEEEECCCCEEHHHHHHH
ILSWCVPIAAFMSGTPLVFPGSSVSAPSLAHIIATAHPRVAHGVPTLWIQLMVHYMRHSP
HHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCC
ERMSLQEIYVGGSAVPAILIKLWEERYGVDVVHVWGMTETVAIGTVARPPSGVSGETRLN
CCCCHHHHEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCEEEE
YRISQGRFPATLEYRVVNDGEIMSSTDRNQGEIQVRGNWVTEHYYHSATEDNGGASSMFR
EEECCCCCCCEEEEEEECCCCEECCCCCCCCEEEEECCEEEHHHCCCCCCCCCCHHHHHC
DHEVEDAPEQFTEDGWLRTGDVGSVTRDGYLTIHDRARDVIRSGGEWIYSTMLENEIMAA
CCCCCCCHHHHCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
TVVVEAAVIGYPDPKWGERPLSVTVLAAGVEPNKETAERLREGLRDTFPNWMLPEYWTFV
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
KSIDKTSVGKFDKIDLRQHLADGDFEIIALKGPGRSSHSDSAE
HHHCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]