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The map label for this gene is dacB [H]

Identifier: 37680905

GI number: 37680905

Start: 2759545

End: 2760975

Strand: Direct

Name: dacB [H]

Synonym: VV2721

Alternate gene names: 37680905

Gene position: 2759545-2760975 (Clockwise)

Preceding gene: 37680904

Following gene: 37680906

Centisome position: 82.26

GC content: 46.75

Gene sequence:

>1431_bases
ATGCGTTTTCTCGCTTCACTTTTCACATCGAGCTTTTGCTTCTTGCTTGCTACTTCTTCCACCATTCAGGCAGAAGAATA
CTCACCGCTAAGCAACCTCCCAGATGGCAGTAGAAGCAGTATTTGGGTAGAAGACTTACAAAACCAAACCTCTTGGCAAA
CCTTAGCGCAAAATACCCGCTTTTTCCCTCCAGCAAGCACACTCAAACTGGCCACCGCCCTAGCGGCCAAACTGGAACTT
GAAGACGACTTTGCCTTCAGCACCGAACTGTTAAGCAATGGCCGTGACATCAGCATTCGTTTCTCTGGCGATCCCACGCT
AAAAACCGCACATTTAAAACAGCTTTTAGAAGCCTACAAGCAGCAGAATGGCGCGAACATTCGTGGAGACATTTGGCTCG
ATAACTCGGTGTTTACAGGCTATGAGCGCGCTGTAGGTTGGCCTTGGGATATTTTGGGCGTTTGCTACAGCGCCCCTTCA
AGTGCCATCACATTAGATGAGAACTGCGCTCAAGCCTCTATTTACACATTGGCCGATGGCAATACTCGAGTTTACGTGCC
AGAGCAGTACCCGATTCACGTCACAACCAATGCGAAAACCGTCTCTAAAGCGATCCAGCAGAGTAATCATTGCGACTTAG
AGCTGTTGACGGGGGATAACAACCAATATCAATTGCAAGGCTGTCTGGTTGAGAGAGACAAACCGTTGCCCCTTAAGTTC
GCGATTCAAGACTCAACTCAATACACGAGTCGAACCTTATATACCTTGTTGAGACAGCTCAAAATTGAACTTGGTGGAGA
AATTAAAATTGGTGTCATGCCAAATAAAGCCAATGTCATTGCCAGCCACTCTTCAGCTACCTTGCCAGAGTTATTAGACA
CTATGCTAAAGGACTCTGATAATCTTATTGCCGACAACCTTACTAAAGCGATTGGGGCTCGCTTTTACTTGCAACCCGGT
AGCTTTGCCAATGGCACTGAAGCGGTGAAACAGATCCTTTATTCCAAAGCGGGAATCGATCTTTCTCGCGCTCAACTCGC
CGACGGTTCGGGCTTGTCGCGTAATAATCGGATCAATCCTATGGATATGATGTCGATCTTGAAATACATCTGGCAACACG
ACAAAGAGCTCGGCTTAGTGGCATTGATGCCCAAATCCGGAGAGTCCGGCACCCTCAAGTACCGCCAAAGCATGCGCAGT
GATCCAGTTAAAGGGCAGATCGTCGCTAAAAGCGGCTCACTTTATGGCAGCTATAACATGGCAGGATTTGTTTTAGATCA
GAATGGACAGCCAAGCGCCGCCTTCATTCAATTTATTACCGATTACTATCCTGATAAAAGTGAAGAGAGTGGCAATGTTG
CACCTATCACACGCTTCGAGCGTGAGTTTTATCGAGACTTAGTTGAGTTTACACAAGTAAAACACAACTAA

Upstream 100 bases:

>100_bases
TTCTAATTTCAGCATTAACTGAATCGCAAACGCCCGCAACTAGCGGGCGTTTTTTATCCTCGCTATAATCAAGCCATTCT
AGATAGATTGGGTTGTCACT

Downstream 100 bases:

>100_bases
CGAAAAACAGCACAAGTTGCCATTTAAGCTAAACCCCAGCCATACTTGTTGATATTTGGCTGGGGAATATCATCATGAAA
CATCACCCAATAAAAGACAA

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRFLASLFTSSFCFLLATSSTIQAEEYSPLSNLPDGSRSSIWVEDLQNQTSWQTLAQNTRFFPPASTLKLATALAAKLEL
EDDFAFSTELLSNGRDISIRFSGDPTLKTAHLKQLLEAYKQQNGANIRGDIWLDNSVFTGYERAVGWPWDILGVCYSAPS
SAITLDENCAQASIYTLADGNTRVYVPEQYPIHVTTNAKTVSKAIQQSNHCDLELLTGDNNQYQLQGCLVERDKPLPLKF
AIQDSTQYTSRTLYTLLRQLKIELGGEIKIGVMPNKANVIASHSSATLPELLDTMLKDSDNLIADNLTKAIGARFYLQPG
SFANGTEAVKQILYSKAGIDLSRAQLADGSGLSRNNRINPMDMMSILKYIWQHDKELGLVALMPKSGESGTLKYRQSMRS
DPVKGQIVAKSGSLYGSYNMAGFVLDQNGQPSAAFIQFITDYYPDKSEESGNVAPITRFEREFYRDLVEFTQVKHN

Sequences:

>Translated_476_residues
MRFLASLFTSSFCFLLATSSTIQAEEYSPLSNLPDGSRSSIWVEDLQNQTSWQTLAQNTRFFPPASTLKLATALAAKLEL
EDDFAFSTELLSNGRDISIRFSGDPTLKTAHLKQLLEAYKQQNGANIRGDIWLDNSVFTGYERAVGWPWDILGVCYSAPS
SAITLDENCAQASIYTLADGNTRVYVPEQYPIHVTTNAKTVSKAIQQSNHCDLELLTGDNNQYQLQGCLVERDKPLPLKF
AIQDSTQYTSRTLYTLLRQLKIELGGEIKIGVMPNKANVIASHSSATLPELLDTMLKDSDNLIADNLTKAIGARFYLQPG
SFANGTEAVKQILYSKAGIDLSRAQLADGSGLSRNNRINPMDMMSILKYIWQHDKELGLVALMPKSGESGTLKYRQSMRS
DPVKGQIVAKSGSLYGSYNMAGFVLDQNGQPSAAFIQFITDYYPDKSEESGNVAPITRFEREFYRDLVEFTQVKHN
>Mature_476_residues
MRFLASLFTSSFCFLLATSSTIQAEEYSPLSNLPDGSRSSIWVEDLQNQTSWQTLAQNTRFFPPASTLKLATALAAKLEL
EDDFAFSTELLSNGRDISIRFSGDPTLKTAHLKQLLEAYKQQNGANIRGDIWLDNSVFTGYERAVGWPWDILGVCYSAPS
SAITLDENCAQASIYTLADGNTRVYVPEQYPIHVTTNAKTVSKAIQQSNHCDLELLTGDNNQYQLQGCLVERDKPLPLKF
AIQDSTQYTSRTLYTLLRQLKIELGGEIKIGVMPNKANVIASHSSATLPELLDTMLKDSDNLIADNLTKAIGARFYLQPG
SFANGTEAVKQILYSKAGIDLSRAQLADGSGLSRNNRINPMDMMSILKYIWQHDKELGLVALMPKSGESGTLKYRQSMRS
DPVKGQIVAKSGSLYGSYNMAGFVLDQNGQPSAAFIQFITDYYPDKSEESGNVAPITRFEREFYRDLVEFTQVKHN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=481, Percent_Identity=35.3430353430353, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52855; Mature: 52855

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLASLFTSSFCFLLATSSTIQAEEYSPLSNLPDGSRSSIWVEDLQNQTSWQTLAQNTR
CHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCHHHHHHHHCCC
FFPPASTLKLATALAAKLELEDDFAFSTELLSNGRDISIRFSGDPTLKTAHLKQLLEAYK
CCCCHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QQNGANIRGDIWLDNSVFTGYERAVGWPWDILGVCYSAPSSAITLDENCAQASIYTLADG
HCCCCCEEEEEEECCCEECCHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
NTRVYVPEQYPIHVTTNAKTVSKAIQQSNHCDLELLTGDNNQYQLQGCLVERDKPLPLKF
CEEEEECCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCEEE
AIQDSTQYTSRTLYTLLRQLKIELGGEIKIGVMPNKANVIASHSSATLPELLDTMLKDSD
EECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCC
NLIADNLTKAIGARFYLQPGSFANGTEAVKQILYSKAGIDLSRAQLADGSGLSRNNRINP
CHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCH
MDMMSILKYIWQHDKELGLVALMPKSGESGTLKYRQSMRSDPVKGQIVAKSGSLYGSYNM
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCCEEECCCC
AGFVLDQNGQPSAAFIQFITDYYPDKSEESGNVAPITRFEREFYRDLVEFTQVKHN
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFLASLFTSSFCFLLATSSTIQAEEYSPLSNLPDGSRSSIWVEDLQNQTSWQTLAQNTR
CHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCHHHHHHHHCCC
FFPPASTLKLATALAAKLELEDDFAFSTELLSNGRDISIRFSGDPTLKTAHLKQLLEAYK
CCCCHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QQNGANIRGDIWLDNSVFTGYERAVGWPWDILGVCYSAPSSAITLDENCAQASIYTLADG
HCCCCCEEEEEEECCCEECCHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
NTRVYVPEQYPIHVTTNAKTVSKAIQQSNHCDLELLTGDNNQYQLQGCLVERDKPLPLKF
CEEEEECCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCEEE
AIQDSTQYTSRTLYTLLRQLKIELGGEIKIGVMPNKANVIASHSSATLPELLDTMLKDSD
EECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCC
NLIADNLTKAIGARFYLQPGSFANGTEAVKQILYSKAGIDLSRAQLADGSGLSRNNRINP
CHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCH
MDMMSILKYIWQHDKELGLVALMPKSGESGTLKYRQSMRSDPVKGQIVAKSGSLYGSYNM
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCCEEECCCC
AGFVLDQNGQPSAAFIQFITDYYPDKSEESGNVAPITRFEREFYRDLVEFTQVKHN
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]