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The map label for this gene is mnmC

Identifier: 37680614

GI number: 37680614

Start: 2462830

End: 2464848

Strand: Direct

Name: mnmC

Synonym: VV2430

Alternate gene names: 37680614

Gene position: 2462830-2464848 (Clockwise)

Preceding gene: 37680608

Following gene: 37680617

Centisome position: 73.42

GC content: 49.68

Gene sequence:

>2019_bases
ATGACATCCATTACCCATGCAGAACTCGGCTGGAATGAAGTCGGCACACCGGTTTCTGACCAATTTGATGACGTTTACTT
CTCCAATGTGAACGGCCTAGAAGAAACGCGCTATGTGTTTCTTAAACAAAATCTTATTCCTGAAAGATGGCAAGAATTTG
ACCGCCGTCGTTTTGTGATTGGCGAAACGGGTTTTGGCACTGGATTGAATTTCCTCGCGGTTTGGCAGGCGTTCAATGAC
TTTCGCCGTGCAAACCCTGACGCAACACTCAAAGAGTTACATTTTATCAGCTTTGAAAAGTTTCCCCTTAGCAAACAAGA
TTTGATCAAAGCGCATCAAGCTTGGCCTGAGTTGGCGGAACTCGCGGAAAAACTTCATCGTCATTATCCACCTGCTGTTC
CAGAATGTCATCGTATTGTGCTGGACAATGGCGCCGTCACGCTTGATCTGTGGCTGGGCGATATTAAAGATTGCCTACCC
TCGGTGCCTTACGGTGAAGAAGGCATCATCGATACTTGGTTCCTTGATGGGTTTGCCCCAAGTAAAAATCCCGAGATGTG
GAATCAGGATCTTTTCAATGGCATGGCAAAACTGGCGAGATCTGAGTGTCGCGTAGCGACGTTTACCTCTGCAGGTTTTG
TAAGACGAGGCTTGATTGAAGCGGGCTTTGCCATGAAAAAAGTGAAAGGCTTTGGCACCAAGCGAGAAATGATCGCTGGC
TGCATGGAAACAAGACAACCACAAAGTCGCCACGCGCCTTACTTCAATCGCACCTCTGCATCTCATCTCGATTCTATTGC
CATCATTGGCGGAGGGATTGCCAGCGCAGCCCTCGCAAAAGCCTTGGTTCAACGTGGGCAAAAAGTGACCCTCTATTGTA
AACACGCCCAAGCGGCAGAAGGCGCATCGGGTAACCGACAAGGCGCAGTTTACCCTTTACTCAATGGCAGTCATGATGGC
GTTTCACGCGTATTTGCCCCCGCGTTTTTGTTTACTCGCCAGTTTGTAGAGCAAGCCGCACAAGCGCTCACCTTCGATCA
TGATTGGTGTGGCGTGACCCAGTTGATGTGGGATGAAAAATCAACCAACAAGTTGGACAAGATGCTCTCAGGGAACTTTG
CACCCGAGTTGATTCAAAAACTCTCCGCAGAAGAAACCGCAGCAAAAATTGGTTTGCCGATCGACATGGCATCGGTTCAT
TATCCACTTGGCGGCTGGCTATGCCCGGCAGAGCTAACCCAAGCCCTCTTTGCGCAATTGGACACCCTCGACAACTTCAC
GGCCAAATTTGAGCAGAGTGTTGAACAACTCATCTGGGACGAAAGGTCACAGCAATGGCAAGTCCATACTCAGGGCCAGC
ACGATACTTATAGCGCTGTGGTTATTGCCAATGGTCATGAGTTCCAAACCTTCAGCCAAACCGCTGACATTCCGCTTGGC
CAAGTCAAAGGTCAAGTCAGCCATGCCCCAGCTACTGAGACGCTCTCTAAACTCAAAAGTGTGCTTTGTTACGACGGCTA
CATGACTCCCGTCAACCCAAACAATCAGCATCTTTGCATTGGCGCGAGTTACGATCGCCGCCATTTGGATACTGAGTTTG
ATGTCAATGCGCAACAAGAGAATGCCGAGAAACTGACGCAATGTGTCCCAAACCAAGCGTGGGCAAAAGAAGTGGATACA
TCTGGCAATTTATCTCGCCAAGGGATCCGCTGTGTGAGCCGAGATCATCTGCCGTTTGTTGGCAATGTCGGCGACTTCTC
AGCCATTAAGCGTCAATACGCCGATTTGCCGCACACTCAAGCAGAAGAGATTGAGGTAATTTCTCAGTTTCCGAACTTAT
TCTGCCTCCTTGGTCTTGGCTCTCGTGGCTTGAGCTCTGCCCCACTGATGGCTGAGTTGCTCGCCTCGCAAATCTGTAAC
GACCCGCTGCCTTTGCCTGTTGACGTGCTTGAAGAGTTACACCCAAGCCGCATGTGGGTAAGAAAACTGCGCAAAGGCAA
AGCCATTACCGAGCTGTAA

Upstream 100 bases:

>100_bases
AGATAATAACTAAGAAGCATAACAAAAGTGGTCAGCTTTCCCAGAAATTCGTACAATCGCCCTATTCTAACCGCGCTACA
TCACAGATTTACATCCTACT

Downstream 100 bases:

>100_bases
GTTGAAGATACGCACAAACAAAAACGCCTCGTTGTATTGACGAGGCGTTTTTATTGAAACAGAGGTTAAGCTTGCGCTTG
GAGCTCTTGCCACAAGTCGC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFND
FRRANPDATLKELHFISFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLP
SVPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CMETRQPQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGSHDG
VSRVFAPAFLFTRQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSAEETAAKIGLPIDMASVH
YPLGGWLCPAELTQALFAQLDTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
QVKGQVSHAPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAEKLTQCVPNQAWAKEVDT
SGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICN
DPLPLPVDVLEELHPSRMWVRKLRKGKAITEL

Sequences:

>Translated_672_residues
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFND
FRRANPDATLKELHFISFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLP
SVPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CMETRQPQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGSHDG
VSRVFAPAFLFTRQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSAEETAAKIGLPIDMASVH
YPLGGWLCPAELTQALFAQLDTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
QVKGQVSHAPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAEKLTQCVPNQAWAKEVDT
SGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICN
DPLPLPVDVLEELHPSRMWVRKLRKGKAITEL
>Mature_671_residues
TSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFNDF
RRANPDATLKELHFISFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPS
VPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAGC
METRQPQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGSHDGV
SRVFAPAFLFTRQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSAEETAAKIGLPIDMASVHY
PLGGWLCPAELTQALFAQLDTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLGQ
VKGQVSHAPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAEKLTQCVPNQAWAKEVDTS
GNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICND
PLPLPVDVLEELHPSRMWVRKLRKGKAITEL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=52.3952095808383, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74970; Mature: 74838

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVI
CCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHEEHHHHCCCHHHHHHHHCCEEEE
GETGFGTGLNFLAVWQAFNDFRRANPDATLKELHFISFEKFPLSKQDLIKAHQAWPELAE
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHH
LAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPSVPYGEEGIIDTWFLDGFAP
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
SKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
CMETRQPQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAE
HHHHCCCHHHCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GASGNRQGAVYPLLNGSHDGVSRVFAPAFLFTRQFVEQAAQALTFDHDWCGVTQLMWDEK
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
STNKLDKMLSGNFAPELIQKLSAEETAAKIGLPIDMASVHYPLGGWLCPAELTQALFAQL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCEECHHHHHHHHHHHH
DTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHH
QVKGQVSHAPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQE
HHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHCCCCCCCCCCHH
NAEKLTQCVPNQAWAKEVDTSGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQ
HHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICNDPLPLPVDVLEELHPSRMWV
HHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHH
RKLRKGKAITEL
HHHHCCCCCCCC
>Mature Secondary Structure 
TSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVI
CCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHEEHHHHCCCHHHHHHHHCCEEEE
GETGFGTGLNFLAVWQAFNDFRRANPDATLKELHFISFEKFPLSKQDLIKAHQAWPELAE
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHH
LAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPSVPYGEEGIIDTWFLDGFAP
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
SKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
CMETRQPQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAE
HHHHCCCHHHCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GASGNRQGAVYPLLNGSHDGVSRVFAPAFLFTRQFVEQAAQALTFDHDWCGVTQLMWDEK
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
STNKLDKMLSGNFAPELIQKLSAEETAAKIGLPIDMASVHYPLGGWLCPAELTQALFAQL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCEECHHHHHHHHHHHH
DTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHH
QVKGQVSHAPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQE
HHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHCCCCCCCCCCHH
NAEKLTQCVPNQAWAKEVDTSGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQ
HHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICNDPLPLPVDVLEELHPSRMWV
HHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHH
RKLRKGKAITEL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA