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The map label for this gene is pflB [H]

Identifier: 37680526

GI number: 37680526

Start: 2368706

End: 2370982

Strand: Direct

Name: pflB [H]

Synonym: VV2342

Alternate gene names: 37680526

Gene position: 2368706-2370982 (Clockwise)

Preceding gene: 37680525

Following gene: 37680527

Centisome position: 70.61

GC content: 46.68

Gene sequence:

>2277_bases
ATGGCAGAGCAATTTGCTAAAGCTTGGGAAGGTTTTGCGGCAGGTGACTGGCAAAACGAAGTAAACGTTCGTGATTTCAT
TCAGAAGAACTACACTCCGTACGAAGGCGACGAATCTTTCCTAGTTTCTGAAGGTACTGAAGCAACCAACAAGCTTTGGG
CTAAGGTAATGGAAGGTATCAAAATTGAGAACTCAACTCACGCTCCAGTTGATTTCGATACTGACCTAATCTCTACCATC
ACTGCTCACGATGCAGGCTACATTGAAAAAGATCTAGAGACTATCGTTGGTCTACAAACTGACGCACCTCTAAAACGCGC
TATCATCCCTAACGGTGGTATCCGCATGGTTGAGGGTTCTTGTAAAGTTTACGGTCGTGAACTCGATCCTCAAATCTCTA
AAATCTACTCTGAATACCGCAAAACACACAACGCTGGCGTATTCGATATCTACACTCCAGATATCCTAGCTTGTCGTAAG
TCTGGTGTTCTAACTGGTCTTCCAGACGCTTACGGCCGTGGTCGTATCATCGGTGACTACCGTCGTGTTGCACTTTACGG
TATCGACTTCCTAATGAAGGACAAACTGGCTCAATTCAAGTCTCTACAAGAGCGTTTTGAGAACGGTGAAGATCTTCAAA
TGACGATGCAACTTCGTGAAGAGATTGCTGAGCAACACCGCGCTCTAGGTCAAATGAAAGTGATGGCAGCGAAATACGGT
TACGATATTTCTCGCCCAGCTGAAACTGCACAAGAAGCTATCCAGTGGACTTACTTCGGCTACCTAGCGGCTGTTAAGTC
TCAAAACGGTGCTGCAATGTCTCTAGGCCGTACTTCTACGTTCCTAGACGTGTACATCGAGCGTGATATCGCTGCTGGCA
AGATCACTGAAGTTGAAGCTCAGGAAATGATCGACCACTTCGTAATGAAACTACGTATGGTTCGTTTCCTACGTACTCCT
GAGTACGATGATCTATTCTCTGGCGACCCAATCTGGGCAACAGAATCTATGGGTGGTATGGGTCTTGACGGTCGTACGCT
AGTAACGCGTTCAAACTTCCGTTTCCTAAACAGCCTATACACAATGGGTCCTTCTCCAGAGCCAAACATCACTGTACTTT
GGTCTGAGCAACTACCTGAAGGCTTCAAGAAGTTCTGTGCGAAAGTATCTATCGATACTTCTTCAATCCAGTACGAAAAC
GACGACCTAATGCGTCCAGACATGAACTCTGACGACTACGCAATCGCATGTTGTGTATCTCCAATGGTTGTTGGTAAGCA
AATGCAGTTCTTCGGCGCTCGTGCAAACCTTGCAAAAACGATGCTTTACGCAATCAACGGTGGTGTTGATGAGAAGCTGA
AAATGCAAGTTGGCCCAGTAATGCCTAAGATCACTTCAGAAGTCCTAAGCTTCGACGAAGTGTGGAACAACCTAGACCAC
TTCATGGACTGGCTAGCGAAGCAATACGTTGCTGCACTAAACGCAATCCACTTCATGCACGACAAGTACAGCTATGAGTC
TGCACTAATGGCACTACACGATCGTGACGTTTACCGCACGATGGCTTGTGGCATCGCTGGTCTATCTATCGCAGCTGACT
CACTATCTGCAATCAAGTACGCGAAAGTGAAACCAATCCGTGACGAAGATGGCGTAGCTATCGACTTCGAAATCGAAGGC
GACTACCCTAAATTCGGTAACAACGATGCTCGCGTAGATGACATCGCTTGTGAACTTGTTGAAGTGTTCATGAACAAGAT
CCGTAAGCTTAAGACTTACCGTGATGCTGTTCCTACTCAGTCTATCCTAACTATCACATCAAACGTGGTATACGGTAAGA
AGACAGGTAATACGCCAGACGGTCGTCGCGCAGGTGCTCCATTTGCACCAGGTGCAAACCCAATGCACGGTCGTGATGAG
AAAGGTGCTGTAGCGTCTCTAACTTCAGTAGGTAAACTGCCATTTGCTCACGCGAAAGACGGTATCTCTTACACCTTCTC
TATCGTACCTAACGCACTTGGTAAAGATGAGAACGCTCAACGCGCTAACCTTGCTGGTCTGATGGATGGTTACTTCCACC
ACGAAGCTGGCATCGAAGGCGGTCAACACTTGAACGTAAACGTTCTAAACCGTGACACGCTTCTAGACGCAGTTAAGCAC
CCTGAGAAGTACCCTCAGCTAACTATCCGTGTATCTGGATACGCAGTTCGCTTTAACTCTCTAACTGCAGAGCAACAAGC
TGACGTTATCGCACGTACTTTCACTGAATCTCTATAA

Upstream 100 bases:

>100_bases
TTGTTATTCATAAGAGTGTACTGCTCCTCTTTAATATGAGGATGGCTGGCTCAACAGCCACAACAGTACGTGTTTTTTCT
ACTAAAAAGGTAGGTATGTC

Downstream 100 bases:

>100_bases
TCTAGAGCTCAGTTAGCAAAACCGGCACTCAGTTGCCGGTTTTTTATTTTTTTGGGAGTAGAACCTCCTACCTTACTTTC
TTCCTTCCTCGCTCGCTCAC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGIKIENSTHAPVDFDTDLISTI
TAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKVYGRELDPQISKIYSEYRKTHNAGVFDIYTPDILACRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQERFENGEDLQMTMQLREEIAEQHRALGQMKVMAAKYG
YDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDIAAGKITEVEAQEMIDHFVMKLRMVRFLRTP
EYDDLFSGDPIWATESMGGMGLDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYEN
DDLMRPDMNSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVMPKITSEVLSFDEVWNNLDH
FMDWLAKQYVAALNAIHFMHDKYSYESALMALHDRDVYRTMACGIAGLSIAADSLSAIKYAKVKPIRDEDGVAIDFEIEG
DYPKFGNNDARVDDIACELVEVFMNKIRKLKTYRDAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENAQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKH
PEKYPQLTIRVSGYAVRFNSLTAEQQADVIARTFTESL

Sequences:

>Translated_758_residues
MAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGIKIENSTHAPVDFDTDLISTI
TAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKVYGRELDPQISKIYSEYRKTHNAGVFDIYTPDILACRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQERFENGEDLQMTMQLREEIAEQHRALGQMKVMAAKYG
YDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDIAAGKITEVEAQEMIDHFVMKLRMVRFLRTP
EYDDLFSGDPIWATESMGGMGLDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYEN
DDLMRPDMNSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVMPKITSEVLSFDEVWNNLDH
FMDWLAKQYVAALNAIHFMHDKYSYESALMALHDRDVYRTMACGIAGLSIAADSLSAIKYAKVKPIRDEDGVAIDFEIEG
DYPKFGNNDARVDDIACELVEVFMNKIRKLKTYRDAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENAQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKH
PEKYPQLTIRVSGYAVRFNSLTAEQQADVIARTFTESL
>Mature_757_residues
AEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGIKIENSTHAPVDFDTDLISTIT
AHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGSCKVYGRELDPQISKIYSEYRKTHNAGVFDIYTPDILACRKS
GVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQERFENGEDLQMTMQLREEIAEQHRALGQMKVMAAKYGY
DISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDIAAGKITEVEAQEMIDHFVMKLRMVRFLRTPE
YDDLFSGDPIWATESMGGMGLDGRTLVTRSNFRFLNSLYTMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYEND
DLMRPDMNSDDYAIACCVSPMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVMPKITSEVLSFDEVWNNLDHF
MDWLAKQYVAALNAIHFMHDKYSYESALMALHDRDVYRTMACGIAGLSIAADSLSAIKYAKVKPIRDEDGVAIDFEIEGD
YPKFGNNDARVDDIACELVEVFMNKIRKLKTYRDAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEK
GAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENAQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKHP
EKYPQLTIRVSGYAVRFNSLTAEQQADVIARTFTESL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=82.7176781002639, Blast_Score=1340, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=754, Percent_Identity=74.1379310344828, Blast_Score=1221, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=595, Percent_Identity=27.3949579831933, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790388, Length=644, Percent_Identity=24.8447204968944, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=87.5, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84494; Mature: 84363

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGI
CCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCE
KIENSTHAPVDFDTDLISTITAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGS
EECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKVYGRELDPQISKIYSEYRKTHNAGVFDIYTPDILACRKSGVLTGLPDAYGRGRIIGDY
CEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLMKDKLAQFKSLQERFENGEDLQMTMQLREEIAEQHRALGQMKVMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDIAAGKITEVEA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDDLFSGDPIWATESMGGMGLDGRTLVTRSNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDMNSDDYAIACCVS
HCCCCCCCCEEEEECHHCHHHHHHHHHEEECCCCCEEECCCCCCCCCCCCCCCEEEEECC
PMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVMPKITSEVLSFDEVWNNLDH
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FMDWLAKQYVAALNAIHFMHDKYSYESALMALHDRDVYRTMACGIAGLSIAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKVKPIRDEDGVAIDFEIEGDYPKFGNNDARVDDIACELVEVFMNKIRKLKTYRDAVPTQ
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKD
HHHEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCC
GISYTFSIVPNALGKDENAQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKH
CCEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHC
PEKYPQLTIRVSGYAVRFNSLTAEQQADVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGI
CHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCE
KIENSTHAPVDFDTDLISTITAHDAGYIEKDLETIVGLQTDAPLKRAIIPNGGIRMVEGS
EECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECC
CKVYGRELDPQISKIYSEYRKTHNAGVFDIYTPDILACRKSGVLTGLPDAYGRGRIIGDY
CEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLMKDKLAQFKSLQERFENGEDLQMTMQLREEIAEQHRALGQMKVMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDIAAGKITEVEA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHH
QEMIDHFVMKLRMVRFLRTPEYDDLFSGDPIWATESMGGMGLDGRTLVTRSNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDMNSDDYAIACCVS
HCCCCCCCCEEEEECHHCHHHHHHHHHEEECCCCCEEECCCCCCCCCCCCCCCEEEEECC
PMVVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPVMPKITSEVLSFDEVWNNLDH
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FMDWLAKQYVAALNAIHFMHDKYSYESALMALHDRDVYRTMACGIAGLSIAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKVKPIRDEDGVAIDFEIEGDYPKFGNNDARVDDIACELVEVFMNKIRKLKTYRDAVPTQ
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKD
HHHEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCC
GISYTFSIVPNALGKDENAQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKH
CCEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHC
PEKYPQLTIRVSGYAVRFNSLTAEQQADVIARTFTESL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]