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The map label for this gene is sigW [H]

Identifier: 37521308

GI number: 37521308

Start: 1843741

End: 1844385

Strand: Reverse

Name: sigW [H]

Synonym: gll1739

Alternate gene names: 37521308

Gene position: 1844385-1843741 (Counterclockwise)

Preceding gene: 37521311

Following gene: 37521307

Centisome position: 39.59

GC content: 62.79

Gene sequence:

>645_bases
ATGGAAAACAGTCTGCCTCTGCCCTGGCCCCTCGTCTCCGCCGCCAAGGAGCCCCTCTGCCTGCACAAAATGAGCAACCA
GGAACTGGTGGTGCGCTGCCAGCAAGGGTTCAGCCCCGACCGGGCGGCCTTTGCCGAACTAATGCGCCGCCACCAAGCCC
ACATCGACCGATTGCTCTACCATCTGGCACCCGACTGGCAGGACCGCTCGGACCTTGCCCAGGAGGTCTGGATTCGTGTC
TACCGCTATGTCAAGCGCCTCAAAGAACCCGAAAAGTTTCGCTCCTGGGCAGGTCGAATCGCCACCAACCTGTTCTACGA
CGAGTTGCGGCGGCGGCGGCGCGGCTGGCCGCCTCTATCGCTCGACGCACCCATCCAGACCAAGGACGGCGAACTGGACT
GGGAGCTTGCCGCCCAGTCCCCCGGGCCGGATGAGAATCTGGTAACGTGTGAATTCTATGAGCACCTGCGGCGGGCAATA
GCCGAGCTGCCGGAAGCTTTTCGGACCACCATCGTCCTTCGAGAAATCGAGGGGATGGCCTACGAAGAAATCGCCGAGAC
CCTGGGTATCTCCGTCGGCACCGTCAAGTCCCGCATCGCCCGGGCCCGGCGCCGTCTGCAGTCGCAACTGAACGCTTACC
TGTAA

Upstream 100 bases:

>100_bases
GGCGGCTTGCAAATCTTTTGCGGATAATCGAGGAACCCGGGTTGTAGAGGGCACGTCACTTCTGGCATTGTCCGCGAACT
TCACCCGGCCTCCCGAATCG

Downstream 100 bases:

>100_bases
GCTCCCCTGCCCCCTGTAATTCCATGGACCCGCATCGCTTCGAACAACTGAGCGCTTATCTGGACGGCGAACTGTCCACC
GCCGAACAACAACAGGTCGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MENSLPLPWPLVSAAKEPLCLHKMSNQELVVRCQQGFSPDRAAFAELMRRHQAHIDRLLYHLAPDWQDRSDLAQEVWIRV
YRYVKRLKEPEKFRSWAGRIATNLFYDELRRRRRGWPPLSLDAPIQTKDGELDWELAAQSPGPDENLVTCEFYEHLRRAI
AELPEAFRTTIVLREIEGMAYEEIAETLGISVGTVKSRIARARRRLQSQLNAYL

Sequences:

>Translated_214_residues
MENSLPLPWPLVSAAKEPLCLHKMSNQELVVRCQQGFSPDRAAFAELMRRHQAHIDRLLYHLAPDWQDRSDLAQEVWIRV
YRYVKRLKEPEKFRSWAGRIATNLFYDELRRRRRGWPPLSLDAPIQTKDGELDWELAAQSPGPDENLVTCEFYEHLRRAI
AELPEAFRTTIVLREIEGMAYEEIAETLGISVGTVKSRIARARRRLQSQLNAYL
>Mature_214_residues
MENSLPLPWPLVSAAKEPLCLHKMSNQELVVRCQQGFSPDRAAFAELMRRHQAHIDRLLYHLAPDWQDRSDLAQEVWIRV
YRYVKRLKEPEKFRSWAGRIATNLFYDELRRRRRGWPPLSLDAPIQTKDGELDWELAAQSPGPDENLVTCEFYEHLRRAI
AELPEAFRTTIVLREIEGMAYEEIAETLGISVGTVKSRIARARRRLQSQLNAYL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=189, Percent_Identity=33.8624338624339, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25010; Mature: 25010

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00622 HTH_LUXR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSLPLPWPLVSAAKEPLCLHKMSNQELVVRCQQGFSPDRAAFAELMRRHQAHIDRLLY
CCCCCCCCCHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HLAPDWQDRSDLAQEVWIRVYRYVKRLKEPEKFRSWAGRIATNLFYDELRRRRRGWPPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAPIQTKDGELDWELAAQSPGPDENLVTCEFYEHLRRAIAELPEAFRTTIVLREIEGMA
CCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
YEEIAETLGISVGTVKSRIARARRRLQSQLNAYL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENSLPLPWPLVSAAKEPLCLHKMSNQELVVRCQQGFSPDRAAFAELMRRHQAHIDRLLY
CCCCCCCCCHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
HLAPDWQDRSDLAQEVWIRVYRYVKRLKEPEKFRSWAGRIATNLFYDELRRRRRGWPPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDAPIQTKDGELDWELAAQSPGPDENLVTCEFYEHLRRAIAELPEAFRTTIVLREIEGMA
CCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
YEEIAETLGISVGTVKSRIARARRRLQSQLNAYL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]