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The map label for this gene is narB [H]

Identifier: 37521140

GI number: 37521140

Start: 1691588

End: 1693666

Strand: Direct

Name: narB [H]

Synonym: gvip218

Alternate gene names: 37521140

Gene position: 1691588-1693666 (Clockwise)

Preceding gene: 37521139

Following gene: 37521141

Centisome position: 36.31

GC content: 66.91

Gene sequence:

>2079_bases
TTGCCGACTGACACCCTGGCCAAAACTCTGTGTCCTTACTGCGGTGTGGGCTGTGGCCTGGAGGTGACCGCGGATGCGCG
CGTGCGCGGCGACCGCGCCCATCCCTCCACTCTGGGCATGGTCTGCGTCAAAGGGGCGACGGTGCTCGAATCGATCGGCA
AAGATCGCCTGCTCTACCCGATGGTGCGCGCTCGCCTCGATGAACCTTTCAGGCAAGCCAGTTGGGAAGAAGCGCTCGCT
CTGGTGGTCGGGAGGTTGCGCGCCGCTTCCCCGGAGAGCCTGTGCTTCTACGGCTCCGGGCAGTTTGTCACAGAAGACTA
TTACGTCGCCCAGAAGCTCTTCAAAGGCTGCCTGGGAAGCAATCATATCGACGCCAACTCGCGACTTTGCATGTCTTCGG
CGGTGTCGGGTTACCTACAGAGCCTGGGTAGCGACGGCCCGCCCGCCTGCTACGACGATCTCGATCTGACCGATTGCGCC
TTTTTGGTGGGCACCAACACCGCCGAGTGCCACCCGGTGCTCTTCAACCGCCTGCTCAAACATCGCAAGCAAGACCTCAA
TTCCCGGCTGGTGGTGGTCGATCCGCGCGCCACGCCCACCGCCAAGGCGGCGGATCTGCACCTGGCCATCCGACCGGGCA
GCGACATCGATCTATTCAACGGCATCGCCCACCTGATCTTGCAGTGGGATCGCGCCGACCAGCGCTTTATCGGCGCCCAC
ACCCAGGGATTCGCGGCAATGGCCGAGGTGGTGCGCCACTACCCGCCCGAGGCGGTCGCCCGCCGCTGCGGGATCACTAC
CGCGGCCCTGGAACTGGCCGCGCGCCTGTGGGTAAATTCGGCGCGCGTCCTTTCGCTCTGGTCGATGGGCATCAATCAGT
CGATCGAAGGCACCGCCAAGGTGCGCGCCCTCATCAACTTGCACCTGCTCACCGCTCAGATCGGTCGGCCCGGATCGGGA
CCCTTCTCGCTCACCGGCCAGCCCAACGCCATGGGCGGGCGCGAGGCCGGCGGATTGTCGCAATTGCTGCCGGGTTACCG
CAGCGTCGCAGACCCCGATCACCGCCGGCAAGTCGAAGAACACTGGCAGTTGCCGTCCGGCAGCATCGCGGCGCGGCCGG
GGCGCACCGCCTGGGAGATGATCGAGGCGCTGGAGGCGGGCGCAGTCGAAGTCTTCTGGATCGCCGCCACCAACCCGGCG
GTGAGCCTGCCGGATCTGGAGCGCACCAAGGCCGCCCTGCTGCGCTCGCCCTTTACGGTCTACCAGGACTGCTACTATCC
GACCGAGACGGCCCGCTACGCCCACGTGTTGCTGCCTGCCACCCAGTGGAGCGAAAAGACCGGCACGATGACCAATTCCG
AGCGCCGCGTCACCCTCTGCCCGGCTTTTCACCCTCCCCCGGGCGAGGCGCGCGACGACTGGCAAATTTTTGCCGAGGTC
GGCCGCCGTCTGGGCTTCGCCGAGCAGTTCGCCTTTGCCGACGCCGCGGCGGTGTTTGCCGAATTTGCCGCCCTCACCCG
GGGGCGCCCCTGCGAGATGACCGGCATCTCCCACGCCCGCCTGGAGCGCGAGGGACCGTTGCAGTGGCCCTGCCCGGCGG
GTTCGGCGGGCACAGCGCGGCTTTACACCGATTGGCGCTTTGCGACACCCGATGCGCGCGCCCGCTTCGCCGCCTGCCAC
GCCCGGGGCCTCGCCGAACCCCCCGACCCCGAGTATCCCTTTGTGCTCATCAACGGCCGTCTCTACGGCCACTGGCACAC
CCTGACACGCACCGGCCGCATCGAGAAACTGCTCAACCTGTACCCTGAGCCGTTCATCGAAATCCACCCCCGCGACGCGG
GCAAACTCGGTCTCGGCGAAGGCGACTGGGTCGAGGTGCGCTCGCGCCGGGGCGCTATCCGCCTGCCGGCCCGCCTCACC
CTGGCGGTAGCACCGGGCACGGTCTTTGTGCCGATGCACTGGGGGGCGCTCTGGGCGGAGGCGGCCGAGGTCAACGCCCT
CACCCACCCCGAATCAGATCCGATTTCGGGTCAACCGGAAGTCAAAAGTTGTGCAGTACAGCTTTCGGCGCTGCCTTGA

Upstream 100 bases:

>100_bases
AGGCCGCGCTCGCGCGCCGAGATCCTCGAAGACCCGGCCTACTACACCCTCAGAAACCGCATCCTCGAATTTCTCTACGA
CCGCTTTGGAGAACCCGTCC

Downstream 100 bases:

>100_bases
CGAAGTTATGTCGAGTGTTAATGGAGGTAACCGCGATGGCCTTGCAGGTAAAGCTGTTGGAACAGAGTTTTGAAGGCGTC
AAACCGAACGCCCATGCGTT

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MPTDTLAKTLCPYCGVGCGLEVTADARVRGDRAHPSTLGMVCVKGATVLESIGKDRLLYPMVRARLDEPFRQASWEEALA
LVVGRLRAASPESLCFYGSGQFVTEDYYVAQKLFKGCLGSNHIDANSRLCMSSAVSGYLQSLGSDGPPACYDDLDLTDCA
FLVGTNTAECHPVLFNRLLKHRKQDLNSRLVVVDPRATPTAKAADLHLAIRPGSDIDLFNGIAHLILQWDRADQRFIGAH
TQGFAAMAEVVRHYPPEAVARRCGITTAALELAARLWVNSARVLSLWSMGINQSIEGTAKVRALINLHLLTAQIGRPGSG
PFSLTGQPNAMGGREAGGLSQLLPGYRSVADPDHRRQVEEHWQLPSGSIAARPGRTAWEMIEALEAGAVEVFWIAATNPA
VSLPDLERTKAALLRSPFTVYQDCYYPTETARYAHVLLPATQWSEKTGTMTNSERRVTLCPAFHPPPGEARDDWQIFAEV
GRRLGFAEQFAFADAAAVFAEFAALTRGRPCEMTGISHARLEREGPLQWPCPAGSAGTARLYTDWRFATPDARARFAACH
ARGLAEPPDPEYPFVLINGRLYGHWHTLTRTGRIEKLLNLYPEPFIEIHPRDAGKLGLGEGDWVEVRSRRGAIRLPARLT
LAVAPGTVFVPMHWGALWAEAAEVNALTHPESDPISGQPEVKSCAVQLSALP

Sequences:

>Translated_692_residues
MPTDTLAKTLCPYCGVGCGLEVTADARVRGDRAHPSTLGMVCVKGATVLESIGKDRLLYPMVRARLDEPFRQASWEEALA
LVVGRLRAASPESLCFYGSGQFVTEDYYVAQKLFKGCLGSNHIDANSRLCMSSAVSGYLQSLGSDGPPACYDDLDLTDCA
FLVGTNTAECHPVLFNRLLKHRKQDLNSRLVVVDPRATPTAKAADLHLAIRPGSDIDLFNGIAHLILQWDRADQRFIGAH
TQGFAAMAEVVRHYPPEAVARRCGITTAALELAARLWVNSARVLSLWSMGINQSIEGTAKVRALINLHLLTAQIGRPGSG
PFSLTGQPNAMGGREAGGLSQLLPGYRSVADPDHRRQVEEHWQLPSGSIAARPGRTAWEMIEALEAGAVEVFWIAATNPA
VSLPDLERTKAALLRSPFTVYQDCYYPTETARYAHVLLPATQWSEKTGTMTNSERRVTLCPAFHPPPGEARDDWQIFAEV
GRRLGFAEQFAFADAAAVFAEFAALTRGRPCEMTGISHARLEREGPLQWPCPAGSAGTARLYTDWRFATPDARARFAACH
ARGLAEPPDPEYPFVLINGRLYGHWHTLTRTGRIEKLLNLYPEPFIEIHPRDAGKLGLGEGDWVEVRSRRGAIRLPARLT
LAVAPGTVFVPMHWGALWAEAAEVNALTHPESDPISGQPEVKSCAVQLSALP
>Mature_691_residues
PTDTLAKTLCPYCGVGCGLEVTADARVRGDRAHPSTLGMVCVKGATVLESIGKDRLLYPMVRARLDEPFRQASWEEALAL
VVGRLRAASPESLCFYGSGQFVTEDYYVAQKLFKGCLGSNHIDANSRLCMSSAVSGYLQSLGSDGPPACYDDLDLTDCAF
LVGTNTAECHPVLFNRLLKHRKQDLNSRLVVVDPRATPTAKAADLHLAIRPGSDIDLFNGIAHLILQWDRADQRFIGAHT
QGFAAMAEVVRHYPPEAVARRCGITTAALELAARLWVNSARVLSLWSMGINQSIEGTAKVRALINLHLLTAQIGRPGSGP
FSLTGQPNAMGGREAGGLSQLLPGYRSVADPDHRRQVEEHWQLPSGSIAARPGRTAWEMIEALEAGAVEVFWIAATNPAV
SLPDLERTKAALLRSPFTVYQDCYYPTETARYAHVLLPATQWSEKTGTMTNSERRVTLCPAFHPPPGEARDDWQIFAEVG
RRLGFAEQFAFADAAAVFAEFAALTRGRPCEMTGISHARLEREGPLQWPCPAGSAGTARLYTDWRFATPDARARFAACHA
RGLAEPPDPEYPFVLINGRLYGHWHTLTRTGRIEKLLNLYPEPFIEIHPRDAGKLGLGEGDWVEVRSRRGAIRLPARLTL
AVAPGTVFVPMHWGALWAEAAEVNALTHPESDPISGQPEVKSCAVQLSALP

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=801, Percent_Identity=30.7116104868914, Blast_Score=341, Evalue=1e-94,
Organism=Escherichia coli, GI3868721, Length=701, Percent_Identity=29.9572039942939, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI3868719, Length=620, Percent_Identity=22.9032258064516, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI3868720, Length=627, Percent_Identity=23.1259968102073, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787778, Length=468, Percent_Identity=23.7179487179487, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081797, Length=748, Percent_Identity=22.192513368984, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787870, Length=708, Percent_Identity=23.728813559322, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693196, Length=481, Percent_Identity=24.948024948025, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI171474008, Length=779, Percent_Identity=22.3363286264442, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 75534; Mature: 75403

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDTLAKTLCPYCGVGCGLEVTADARVRGDRAHPSTLGMVCVKGATVLESIGKDRLLYP
CCCHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCHHHHHHHCCHHHHHHCCCCCHHHH
MVRARLDEPFRQASWEEALALVVGRLRAASPESLCFYGSGQFVTEDYYVAQKLFKGCLGS
HHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCC
NHIDANSRLCMSSAVSGYLQSLGSDGPPACYDDLDLTDCAFLVGTNTAECHPVLFNRLLK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHH
HRKQDLNSRLVVVDPRATPTAKAADLHLAIRPGSDIDLFNGIAHLILQWDRADQRFIGAH
HHHHHHCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHEECCCCCCCEECCC
TQGFAAMAEVVRHYPPEAVARRCGITTAALELAARLWVNSARVLSLWSMGINQSIEGTAK
CHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHH
VRALINLHLLTAQIGRPGSGPFSLTGQPNAMGGREAGGLSQLLPGYRSVADPDHRRQVEE
HHHHHHHHEEEHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHH
HWQLPSGSIAARPGRTAWEMIEALEAGAVEVFWIAATNPAVSLPDLERTKAALLRSPFTV
HHCCCCCCEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHH
YQDCYYPTETARYAHVLLPATQWSEKTGTMTNSERRVTLCPAFHPPPGEARDDWQIFAEV
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
GRRLGFAEQFAFADAAAVFAEFAALTRGRPCEMTGISHARLEREGPLQWPCPAGSAGTAR
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEE
LYTDWRFATPDARARFAACHARGLAEPPDPEYPFVLINGRLYGHWHTLTRTGRIEKLLNL
EEECEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEEHHHCHHHHHHHHH
YPEPFIEIHPRDAGKLGLGEGDWVEVRSRRGAIRLPARLTLAVAPGTVFVPMHWGALWAE
CCCCCEEECCCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCEEEEEECCHHHHHH
AAEVNALTHPESDPISGQPEVKSCAVQLSALP
HHHCCCCCCCCCCCCCCCCCHHHHHEEEECCC
>Mature Secondary Structure 
PTDTLAKTLCPYCGVGCGLEVTADARVRGDRAHPSTLGMVCVKGATVLESIGKDRLLYP
CCHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCHHHHHHHCCHHHHHHCCCCCHHHH
MVRARLDEPFRQASWEEALALVVGRLRAASPESLCFYGSGQFVTEDYYVAQKLFKGCLGS
HHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCC
NHIDANSRLCMSSAVSGYLQSLGSDGPPACYDDLDLTDCAFLVGTNTAECHPVLFNRLLK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEECCCCCCCHHHHHHHHH
HRKQDLNSRLVVVDPRATPTAKAADLHLAIRPGSDIDLFNGIAHLILQWDRADQRFIGAH
HHHHHHCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHEECCCCCCCEECCC
TQGFAAMAEVVRHYPPEAVARRCGITTAALELAARLWVNSARVLSLWSMGINQSIEGTAK
CHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHH
VRALINLHLLTAQIGRPGSGPFSLTGQPNAMGGREAGGLSQLLPGYRSVADPDHRRQVEE
HHHHHHHHEEEHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHH
HWQLPSGSIAARPGRTAWEMIEALEAGAVEVFWIAATNPAVSLPDLERTKAALLRSPFTV
HHCCCCCCEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCHHH
YQDCYYPTETARYAHVLLPATQWSEKTGTMTNSERRVTLCPAFHPPPGEARDDWQIFAEV
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
GRRLGFAEQFAFADAAAVFAEFAALTRGRPCEMTGISHARLEREGPLQWPCPAGSAGTAR
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEE
LYTDWRFATPDARARFAACHARGLAEPPDPEYPFVLINGRLYGHWHTLTRTGRIEKLLNL
EEECEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEEEHHHCHHHHHHHHH
YPEPFIEIHPRDAGKLGLGEGDWVEVRSRRGAIRLPARLTLAVAPGTVFVPMHWGALWAE
CCCCCEEECCCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCEEEEEECCHHHHHH
AAEVNALTHPESDPISGQPEVKSCAVQLSALP
HHHCCCCCCCCCCCCCCCCCHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]