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The map label for this gene is rne [H]

Identifier: 37521075

GI number: 37521075

Start: 1620272

End: 1622260

Strand: Direct

Name: rne [H]

Synonym: gvip208

Alternate gene names: 37521075

Gene position: 1620272-1622260 (Clockwise)

Preceding gene: 37521071

Following gene: 37521076

Centisome position: 34.78

GC content: 66.67

Gene sequence:

>1989_bases
ATGCCCAAGCAAATCATCATCGCGGAACAATACCGTCTGGCGGCGGTATTTGCAGAAGATCAAATTCAAGAACTCATCGT
CGCCTCCGGCAACCACCAGGTCGGAGACATTTACCTGGGCATCGTTGAGAACGTGCTGCCCAGCATCGACGCCGCTTTCG
TCAACATCGGCAATCCCGAACGCAACGGCTTCATCCACGTCACCGACCTCGGTCAGATCCGGTTGCGCCGCTCCTCCGCT
TCGATTAGCGAACTGGTCACCCCCCAGCAAAAAGTGCTCGTCCAGGTGATGAAGGAGCCCACCGGCAACAAAGGTCCGCG
CTTGACCGGCAACGTCAGCCTGCCGGGACGCTTTTTGGTGTTGATGCCCTTCGGCCGGGGCGTCAACCTCTCGCGGCGCA
TCCGCGACGACGACGAGCGCAATCGCCTGCGTGCCCTGGCGGTGCTCATCAAACCCCCGGGCATGGGCCTGCTGGTGCGT
ACCGAGGCGGCCGGCATGGACGAGCAGGGCGTTCTCGAAGATCTCGAAAGCCTGCTCAAGCAGTGGGAGAAAATTCAGAA
GCAGACCGCCGAGACCCGGGCTCCGGCCTTGCTCAACCGCGACGAGGACTTTATTCAACGCATCTTGCGCGACGCCTACG
GCGCCCAGGTCAACCGCATCGTGGTGGATTCCCACGTCGGGGTCAAGCGTGTCAAGCAGCACCTGCTCAACTGGAACGGC
GGGCGCATGCCCCAGGGCGTCTACGTCGAGCACCACCGCGAGCCCAGTTCGATTCTCGATTACTTTCGGGTCAACGCGGC
AATTCGCGAGGCGCTGCGGCCCCGCGTCGATTTGCCCTCCGGCGGTTACATCATCATCGAGCCCACCGAGGCGCTCACGG
TGATCGACGTCAACTCCGGTTCCTTCACCCGCTCGGCGACTTCACGCGAGACGGTGCTGTGGACCAACTGCGAGGCGGCC
ACCGAAATCGCCCGCCAACTGCGGTTGCGCAACATCGCGGGCATCATCATCATCGACTTTATCGACATGGACGCGCGCCG
CGACCAGTTGCAGGTGCTGGAGCACTTCTCCAAGGCGCTCAAAGCCGACAAGTCGCGCCCGCAGATCGCTCAGCTCACCG
AACTGGGCCTTGTCGAGATCACCCGCAAGCGTCAGGGCCAGAGCATCTACGAGATCTTCGGCAAGCCCTGCCCCACCTGC
GACGGCCTCGGCATCACCACCCACCTGCCCGGCACTCGCCACGAGCCAGGCACCGAGGCTCTACCCACCTTTGCCCCGTC
CGCCGAGGAACTGGAAGGCTTCGAAGGTGAAGGATTTGCCGGGGCCGGGGCCGGCGGCGAGTACGAAGAGGAAGAATCGT
CGGATCTCGACCTCACGGCCCATCCGGTTTTTCGCAACGACCGGCGCCGCCTGCCGATGGAGCGCCGACCGGCGGCGCCA
CCGCCGGCCGGCAACGGTGCCCCGGGCGGAACCGCCGGTGGACGCAACCGCCGCCGCCGCGGCCGGGGCGGCAACGGCCG
CGGCGGGCCGGAGGAGGGCCTGGGACGTCCCGCGATCGAGACGGGCGAACCGGTTCCCGGCGCGTCCCCGCCTTCGCCGG
CCGCCCGTGCGCCCGAGGCCGAACCGGCGGAGGCAGCGCCTTCGATGCCAGGCTACGGCGGCTACGAAGATCGCCACGAC
GAAGGCGCCCAACCGACGCAGCCTCCGATCGAAGCGCCCGAGATGCCCATCGACCACATCCCTGCCGCCGAGCAGGAAGT
GCTCGCCTACATGGGGCTGCCCGCCGAGCTGTTGAGCGGTCCGGCCATCACCTCGGGCAACACCTCGATCCTCTCGGTCC
GGCCGCCCAGTCCCGACAAACCCGAAGACGAAGGGGGCGAGGAGGGCGAGGGCGATCCGCGCCGCCGCCGCCGCCGCCGC
ACCACCCGCAGCAAAGCCCCCGAGGCGCAGAACACCCCGGAGCCGGTTCCCGCCAATCCGGAGGAATAA

Upstream 100 bases:

>100_bases
TGGCACATCGCTGCCAGGCTGTTTAGTACCTCGCGAAATGAGCCCAATTGCCTGCTGCTGTGACGGACTGAAGGTGCCGT
CACTTTTCACAAGGAGTCCT

Downstream 100 bases:

>100_bases
CATCAAGCGGCCGTCTCAGACTCCCCTGCCTTCGCTGGAGCGCGAGCGCGCCCTATGGGCGGCGGGCATCCGCCGTCTGG
CGGGGGTGGACGAGGTCGGC

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MPKQIIIAEQYRLAAVFAEDQIQELIVASGNHQVGDIYLGIVENVLPSIDAAFVNIGNPERNGFIHVTDLGQIRLRRSSA
SISELVTPQQKVLVQVMKEPTGNKGPRLTGNVSLPGRFLVLMPFGRGVNLSRRIRDDDERNRLRALAVLIKPPGMGLLVR
TEAAGMDEQGVLEDLESLLKQWEKIQKQTAETRAPALLNRDEDFIQRILRDAYGAQVNRIVVDSHVGVKRVKQHLLNWNG
GRMPQGVYVEHHREPSSILDYFRVNAAIREALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATSRETVLWTNCEAA
TEIARQLRLRNIAGIIIIDFIDMDARRDQLQVLEHFSKALKADKSRPQIAQLTELGLVEITRKRQGQSIYEIFGKPCPTC
DGLGITTHLPGTRHEPGTEALPTFAPSAEELEGFEGEGFAGAGAGGEYEEEESSDLDLTAHPVFRNDRRRLPMERRPAAP
PPAGNGAPGGTAGGRNRRRRGRGGNGRGGPEEGLGRPAIETGEPVPGASPPSPAARAPEAEPAEAAPSMPGYGGYEDRHD
EGAQPTQPPIEAPEMPIDHIPAAEQEVLAYMGLPAELLSGPAITSGNTSILSVRPPSPDKPEDEGGEEGEGDPRRRRRRR
TTRSKAPEAQNTPEPVPANPEE

Sequences:

>Translated_662_residues
MPKQIIIAEQYRLAAVFAEDQIQELIVASGNHQVGDIYLGIVENVLPSIDAAFVNIGNPERNGFIHVTDLGQIRLRRSSA
SISELVTPQQKVLVQVMKEPTGNKGPRLTGNVSLPGRFLVLMPFGRGVNLSRRIRDDDERNRLRALAVLIKPPGMGLLVR
TEAAGMDEQGVLEDLESLLKQWEKIQKQTAETRAPALLNRDEDFIQRILRDAYGAQVNRIVVDSHVGVKRVKQHLLNWNG
GRMPQGVYVEHHREPSSILDYFRVNAAIREALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATSRETVLWTNCEAA
TEIARQLRLRNIAGIIIIDFIDMDARRDQLQVLEHFSKALKADKSRPQIAQLTELGLVEITRKRQGQSIYEIFGKPCPTC
DGLGITTHLPGTRHEPGTEALPTFAPSAEELEGFEGEGFAGAGAGGEYEEEESSDLDLTAHPVFRNDRRRLPMERRPAAP
PPAGNGAPGGTAGGRNRRRRGRGGNGRGGPEEGLGRPAIETGEPVPGASPPSPAARAPEAEPAEAAPSMPGYGGYEDRHD
EGAQPTQPPIEAPEMPIDHIPAAEQEVLAYMGLPAELLSGPAITSGNTSILSVRPPSPDKPEDEGGEEGEGDPRRRRRRR
TTRSKAPEAQNTPEPVPANPEE
>Mature_661_residues
PKQIIIAEQYRLAAVFAEDQIQELIVASGNHQVGDIYLGIVENVLPSIDAAFVNIGNPERNGFIHVTDLGQIRLRRSSAS
ISELVTPQQKVLVQVMKEPTGNKGPRLTGNVSLPGRFLVLMPFGRGVNLSRRIRDDDERNRLRALAVLIKPPGMGLLVRT
EAAGMDEQGVLEDLESLLKQWEKIQKQTAETRAPALLNRDEDFIQRILRDAYGAQVNRIVVDSHVGVKRVKQHLLNWNGG
RMPQGVYVEHHREPSSILDYFRVNAAIREALRPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATSRETVLWTNCEAAT
EIARQLRLRNIAGIIIIDFIDMDARRDQLQVLEHFSKALKADKSRPQIAQLTELGLVEITRKRQGQSIYEIFGKPCPTCD
GLGITTHLPGTRHEPGTEALPTFAPSAEELEGFEGEGFAGAGAGGEYEEEESSDLDLTAHPVFRNDRRRLPMERRPAAPP
PAGNGAPGGTAGGRNRRRRGRGGNGRGGPEEGLGRPAIETGEPVPGASPPSPAARAPEAEPAEAAPSMPGYGGYEDRHDE
GAQPTQPPIEAPEMPIDHIPAAEQEVLAYMGLPAELLSGPAITSGNTSILSVRPPSPDKPEDEGGEEGEGDPRRRRRRRT
TRSKAPEAQNTPEPVPANPEE

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=405, Percent_Identity=37.2839506172839, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082235, Length=429, Percent_Identity=36.3636363636364, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 72195; Mature: 72064

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIIIAEQYRLAAVFAEDQIQELIVASGNHQVGDIYLGIVENVLPSIDAAFVNIGNPE
CCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
RNGFIHVTDLGQIRLRRSSASISELVTPQQKVLVQVMKEPTGNKGPRLTGNVSLPGRFLV
CCCEEEEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
LMPFGRGVNLSRRIRDDDERNRLRALAVLIKPPGMGLLVRTEAAGMDEQGVLEDLESLLK
EEECCCCCCHHHHCCCCHHHHHEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEKIQKQTAETRAPALLNRDEDFIQRILRDAYGAQVNRIVVDSHVGVKRVKQHLLNWNG
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCC
GRMPQGVYVEHHREPSSILDYFRVNAAIREALRPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCC
SFTRSATSRETVLWTNCEAATEIARQLRLRNIAGIIIIDFIDMDARRDQLQVLEHFSKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KADKSRPQIAQLTELGLVEITRKRQGQSIYEIFGKPCPTCDGLGITTHLPGTRHEPGTEA
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
LPTFAPSAEELEGFEGEGFAGAGAGGEYEEEESSDLDLTAHPVFRNDRRRLPMERRPAAP
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCCHHCCCCCC
PPAGNGAPGGTAGGRNRRRRGRGGNGRGGPEEGLGRPAIETGEPVPGASPPSPAARAPEA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EPAEAAPSMPGYGGYEDRHDEGAQPTQPPIEAPEMPIDHIPAAEQEVLAYMGLPAELLSG
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCC
PAITSGNTSILSVRPPSPDKPEDEGGEEGEGDPRRRRRRRTTRSKAPEAQNTPEPVPANP
CCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EE
CC
>Mature Secondary Structure 
PKQIIIAEQYRLAAVFAEDQIQELIVASGNHQVGDIYLGIVENVLPSIDAAFVNIGNPE
CCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
RNGFIHVTDLGQIRLRRSSASISELVTPQQKVLVQVMKEPTGNKGPRLTGNVSLPGRFLV
CCCEEEEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
LMPFGRGVNLSRRIRDDDERNRLRALAVLIKPPGMGLLVRTEAAGMDEQGVLEDLESLLK
EEECCCCCCHHHHCCCCHHHHHEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEKIQKQTAETRAPALLNRDEDFIQRILRDAYGAQVNRIVVDSHVGVKRVKQHLLNWNG
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCC
GRMPQGVYVEHHREPSSILDYFRVNAAIREALRPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCCC
SFTRSATSRETVLWTNCEAATEIARQLRLRNIAGIIIIDFIDMDARRDQLQVLEHFSKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
KADKSRPQIAQLTELGLVEITRKRQGQSIYEIFGKPCPTCDGLGITTHLPGTRHEPGTEA
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
LPTFAPSAEELEGFEGEGFAGAGAGGEYEEEESSDLDLTAHPVFRNDRRRLPMERRPAAP
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCCHHCCCCCC
PPAGNGAPGGTAGGRNRRRRGRGGNGRGGPEEGLGRPAIETGEPVPGASPPSPAARAPEA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EPAEAAPSMPGYGGYEDRHDEGAQPTQPPIEAPEMPIDHIPAAEQEVLAYMGLPAELLSG
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCC
PAITSGNTSILSVRPPSPDKPEDEGGEEGEGDPRRRRRRRTTRSKAPEAQNTPEPVPANP
CCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]