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The map label for this gene is slt [H]

Identifier: 37520090

GI number: 37520090

Start: 558625

End: 560628

Strand: Reverse

Name: slt [H]

Synonym: gll0521

Alternate gene names: 37520090

Gene position: 560628-558625 (Counterclockwise)

Preceding gene: 37520091

Following gene: 37520088

Centisome position: 12.03

GC content: 66.97

Gene sequence:

>2004_bases
GTGACACCGATGCCGTTTCGCTTCAAGACCAGGACGCTGCTAATCGCCGCCAGTCTGGCGGGGGCTCTGGGTCTCGCCTG
GGTCGCCTTTCCAAAGCCGCCGACGGAGGATAGTCCCCTGGTGGCCCTCTCCAGTCAGGATCGCTCCGGGCGCTACCGCC
GGGGAAGCGCCGCTTTCGTGCGGGGCGATTACGCCGAGGCCCGCCGGCAACTGGCAGGATTGGCCGCGTCCTACCCGGCC
CTGGCCGAACGCATCGGGTTGAAGCTTGCCGTTGCCAGCGGCGAGGGCCGCCGCTGGCTGGAGGCCCACCCCAAAAGCCC
ACTGGTTCCCGACGCGCTGGCCTTGCTGGCCCGCCAGGATCGTGCCGCTCTGCTCCCACTGCTGAACGGCTATCCGGATC
ACTATCGCACCCGCGAGGCGCTGGAGCGGGCGCTGGTGCGCGACCCCGACGATCACACCCTGGCTGGAGCGCTGCTCGCC
CGTTTTCCCGAGAGCACCCTCGCCTACGCGATGGCGACCCGGCGCGAAAAAATCGGCGAACTCTCCCCGGCCGAATGGCA
GTTGGTGGCTTCTATTTATCGGCGGGTCAACGCCAAACAGGCCCTCGCCGCCTACGAGCGCGCCCCCGCTACCCCCGAAA
ACCTGCTCGAGCGGGCCCGTCTCCAGCGCAAGTTGGGGGACAAACCGGCCGCCCGGGAACTCTACGAACTGGTGTTGCGG
CGCTTCCCCGAATCGCCTCTGGTGCTCGATGCGGCCTTCGAGCGCGCCGAGTTGCTGGGGGCGGGCGAGGCTTTTGCTGC
GCTCGAACCATGGGAGCGCGCCTCCGCCGAGCGCGGCGACGAAGTGCTCTGGGCGCGCAGCCAGATCGCCGCGCGTCGCC
TGGACGCTCCCGAGATGGCCATTCCCTTATACCGGCGGTTGGTGGAGCGCTACCCCCAAAGCGCCAAAGCTCCCAACGCC
GCCTGGGAACTGGCCGCCCAGAGCGCCGAGCGCGGCAATACGGGAGCGGCCCGCTCCTATGCCCGCTGGTTGGTGCGCAA
CCACCCGGCCGATCCGTTTGCGCCCAAGGCGGCTTTTTGGCTCGGCAAGTGGGCCGAGCAGGCCGGGGCCACCGCCGAGG
CCCGCTCCCAATTTCGCGAAGTGCTCAAGCGCTATCCCCGCAGCTACTACGCCTGGCGCTCCGCCGCCCGGCTGGGTCTG
GCCGACGGCAGTTATAACATCGACCAATCCGTCGTCTCGGTGCGCTGGTCGCAGCCTCCCCTGCCGCTGGCGGGTACCTC
CGCCGCCCTGCGCGAACTGCTGGCCCTGGGCGAAATCTCCGAGGCCGAGCAACAGTGGCAGTCGGAGACCTACCGCTGCA
AGCTCTCTCCCACCCAAAAGCTCGCCGAATCGCAACTGCGCGCCCGCCTCGGCCAGCACCTCAGATCGATCAACCAGGCG
ACCCTTACTTTGTTTGCCGAGCCGCCCGCCGACCCGCGCGCCTGGGAGCAGGCCTATCCGCTCTTCTATGCCCCCGGGCT
GCGGCAGTGGTCCCAGGAGCGCAATCTCAATCCGCTGTTGGTCGCATCGCTGATTCGCCAGGAATCGCGCTTCGAGCCTG
CCATTCGTTCGCGCTCCGGGGCGATGGGTCTGATGCAGATTATGCCCGCCACCGCCCGGTTCATCGCCGAGCGCGAACCG
GGGCAATTCGAACTGACCAACCCCGTGGACAATCTGCGCCTGGGCACCTGGTATCTGCGCTATACCCACGAGCGCTTCGG
CGGCAGCACGATGCTGGCGCTCGCCAGTTACAACGCCGGACCCGGCAACGTCTCCAAGTGGCTCAAGCGCAACCCCGTCA
CCGACGAAGAAGATTTTATCGAGCAGATCCCTTTTAAGGAGACGCGCTTCTACGTGGTCAACATCTACGAGAACTACTGG
AACTATCTGCGGTTGTATACCAGCCAGGGCCGCAAAGCTCTAGCCGCAATCGACGGCCGGACGGCTATGGACGGCTCGAA
TTGA

Upstream 100 bases:

>100_bases
CCGACGAATACACGCTCATCACTGCACCGGCTTACCAGGAGCGCATCGCCCGCGCGCTGACCCGCGGGCTGGAGCGTTGG
CTGCACGCCGAGCGGCCGGA

Downstream 100 bases:

>100_bases
TGTACTCAATCAGCGCACGGTCAAAGCCGGCCGGATCCTCAAGGGGGTTTGGGAGCGAAGTACCTTGACGGGCACGCAAC
CTGTCGGCGAACGCTTCGAA

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MTPMPFRFKTRTLLIAASLAGALGLAWVAFPKPPTEDSPLVALSSQDRSGRYRRGSAAFVRGDYAEARRQLAGLAASYPA
LAERIGLKLAVASGEGRRWLEAHPKSPLVPDALALLARQDRAALLPLLNGYPDHYRTREALERALVRDPDDHTLAGALLA
RFPESTLAYAMATRREKIGELSPAEWQLVASIYRRVNAKQALAAYERAPATPENLLERARLQRKLGDKPAARELYELVLR
RFPESPLVLDAAFERAELLGAGEAFAALEPWERASAERGDEVLWARSQIAARRLDAPEMAIPLYRRLVERYPQSAKAPNA
AWELAAQSAERGNTGAARSYARWLVRNHPADPFAPKAAFWLGKWAEQAGATAEARSQFREVLKRYPRSYYAWRSAARLGL
ADGSYNIDQSVVSVRWSQPPLPLAGTSAALRELLALGEISEAEQQWQSETYRCKLSPTQKLAESQLRARLGQHLRSINQA
TLTLFAEPPADPRAWEQAYPLFYAPGLRQWSQERNLNPLLVASLIRQESRFEPAIRSRSGAMGLMQIMPATARFIAEREP
GQFELTNPVDNLRLGTWYLRYTHERFGGSTMLALASYNAGPGNVSKWLKRNPVTDEEDFIEQIPFKETRFYVVNIYENYW
NYLRLYTSQGRKALAAIDGRTAMDGSN

Sequences:

>Translated_667_residues
MTPMPFRFKTRTLLIAASLAGALGLAWVAFPKPPTEDSPLVALSSQDRSGRYRRGSAAFVRGDYAEARRQLAGLAASYPA
LAERIGLKLAVASGEGRRWLEAHPKSPLVPDALALLARQDRAALLPLLNGYPDHYRTREALERALVRDPDDHTLAGALLA
RFPESTLAYAMATRREKIGELSPAEWQLVASIYRRVNAKQALAAYERAPATPENLLERARLQRKLGDKPAARELYELVLR
RFPESPLVLDAAFERAELLGAGEAFAALEPWERASAERGDEVLWARSQIAARRLDAPEMAIPLYRRLVERYPQSAKAPNA
AWELAAQSAERGNTGAARSYARWLVRNHPADPFAPKAAFWLGKWAEQAGATAEARSQFREVLKRYPRSYYAWRSAARLGL
ADGSYNIDQSVVSVRWSQPPLPLAGTSAALRELLALGEISEAEQQWQSETYRCKLSPTQKLAESQLRARLGQHLRSINQA
TLTLFAEPPADPRAWEQAYPLFYAPGLRQWSQERNLNPLLVASLIRQESRFEPAIRSRSGAMGLMQIMPATARFIAEREP
GQFELTNPVDNLRLGTWYLRYTHERFGGSTMLALASYNAGPGNVSKWLKRNPVTDEEDFIEQIPFKETRFYVVNIYENYW
NYLRLYTSQGRKALAAIDGRTAMDGSN
>Mature_666_residues
TPMPFRFKTRTLLIAASLAGALGLAWVAFPKPPTEDSPLVALSSQDRSGRYRRGSAAFVRGDYAEARRQLAGLAASYPAL
AERIGLKLAVASGEGRRWLEAHPKSPLVPDALALLARQDRAALLPLLNGYPDHYRTREALERALVRDPDDHTLAGALLAR
FPESTLAYAMATRREKIGELSPAEWQLVASIYRRVNAKQALAAYERAPATPENLLERARLQRKLGDKPAARELYELVLRR
FPESPLVLDAAFERAELLGAGEAFAALEPWERASAERGDEVLWARSQIAARRLDAPEMAIPLYRRLVERYPQSAKAPNAA
WELAAQSAERGNTGAARSYARWLVRNHPADPFAPKAAFWLGKWAEQAGATAEARSQFREVLKRYPRSYYAWRSAARLGLA
DGSYNIDQSVVSVRWSQPPLPLAGTSAALRELLALGEISEAEQQWQSETYRCKLSPTQKLAESQLRARLGQHLRSINQAT
LTLFAEPPADPRAWEQAYPLFYAPGLRQWSQERNLNPLLVASLIRQESRFEPAIRSRSGAMGLMQIMPATARFIAEREPG
QFELTNPVDNLRLGTWYLRYTHERFGGSTMLALASYNAGPGNVSKWLKRNPVTDEEDFIEQIPFKETRFYVVNIYENYWN
YLRLYTSQGRKALAAIDGRTAMDGSN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=324, Percent_Identity=28.3950617283951, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74759; Mature: 74627

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPMPFRFKTRTLLIAASLAGALGLAWVAFPKPPTEDSPLVALSSQDRSGRYRRGSAAFV
CCCCCCEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEE
RGDYAEARRQLAGLAASYPALAERIGLKLAVASGEGRRWLEAHPKSPLVPDALALLARQD
ECCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHCC
RAALLPLLNGYPDHYRTREALERALVRDPDDHTLAGALLARFPESTLAYAMATRREKIGE
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LSPAEWQLVASIYRRVNAKQALAAYERAPATPENLLERARLQRKLGDKPAARELYELVLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RFPESPLVLDAAFERAELLGAGEAFAALEPWERASAERGDEVLWARSQIAARRLDAPEMA
HCCCCCEEHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
IPLYRRLVERYPQSAKAPNAAWELAAQSAERGNTGAARSYARWLVRNHPADPFAPKAAFW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LGKWAEQAGATAEARSQFREVLKRYPRSYYAWRSAARLGLADGSYNIDQSVVSVRWSQPP
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCC
LPLAGTSAALRELLALGEISEAEQQWQSETYRCKLSPTQKLAESQLRARLGQHLRSINQA
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCHH
TLTLFAEPPADPRAWEQAYPLFYAPGLRQWSQERNLNPLLVASLIRQESRFEPAIRSRSG
EEEEEECCCCCCHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCC
AMGLMQIMPATARFIAEREPGQFELTNPVDNLRLGTWYLRYTHERFGGSTMLALASYNAG
CHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEECCEEEEEHHHHHCCCEEEEEEECCCC
PGNVSKWLKRNPVTDEEDFIEQIPFKETRFYVVNIYENYWNYLRLYTSQGRKALAAIDGR
CCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEHHCCCC
TAMDGSN
CCCCCCC
>Mature Secondary Structure 
TPMPFRFKTRTLLIAASLAGALGLAWVAFPKPPTEDSPLVALSSQDRSGRYRRGSAAFV
CCCCCEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEE
RGDYAEARRQLAGLAASYPALAERIGLKLAVASGEGRRWLEAHPKSPLVPDALALLARQD
ECCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHCC
RAALLPLLNGYPDHYRTREALERALVRDPDDHTLAGALLARFPESTLAYAMATRREKIGE
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LSPAEWQLVASIYRRVNAKQALAAYERAPATPENLLERARLQRKLGDKPAARELYELVLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RFPESPLVLDAAFERAELLGAGEAFAALEPWERASAERGDEVLWARSQIAARRLDAPEMA
HCCCCCEEHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
IPLYRRLVERYPQSAKAPNAAWELAAQSAERGNTGAARSYARWLVRNHPADPFAPKAAFW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LGKWAEQAGATAEARSQFREVLKRYPRSYYAWRSAARLGLADGSYNIDQSVVSVRWSQPP
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCC
LPLAGTSAALRELLALGEISEAEQQWQSETYRCKLSPTQKLAESQLRARLGQHLRSINQA
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCHH
TLTLFAEPPADPRAWEQAYPLFYAPGLRQWSQERNLNPLLVASLIRQESRFEPAIRSRSG
EEEEEECCCCCCHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCC
AMGLMQIMPATARFIAEREPGQFELTNPVDNLRLGTWYLRYTHERFGGSTMLALASYNAG
CHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEECCEEEEEHHHHHCCCEEEEEEECCCC
PGNVSKWLKRNPVTDEEDFIEQIPFKETRFYVVNIYENYWNYLRLYTSQGRKALAAIDGR
CCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEHHCCCC
TAMDGSN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]