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The map label for this gene is walK [H]

Identifier: 37519949

GI number: 37519949

Start: 396973

End: 398040

Strand: Reverse

Name: walK [H]

Synonym: gll0380

Alternate gene names: 37519949

Gene position: 398040-396973 (Counterclockwise)

Preceding gene: 37519952

Following gene: 37519948

Centisome position: 8.54

GC content: 60.77

Gene sequence:

>1068_bases
ATGTACACCGCATTCAAGCTTCAGGAGCCGCGCCGACGGGCGCTGTACGGCATTGTCGCCTTGATCTTGTTTGTCGAAAC
GGTGGAGTATTTCGCTCCAACCGGTTACATATTCAGCTATCTGTACGCCGTGCCAATATTGATTGCCGCCAACCATTTTT
CGGAGCGGCAGACGCAATGGATTACATTTTTGAGCCTGGTTTCGACGATTTTGGGGCTGTTTGTTCCCACCTTTGAACTC
AACAGCCTCACCCTGGTGAACCGATTGATTATCTGCTCGGCCCTCTGGACGGTGGGCACTTTGAGTCTTCGCTACCGCCA
GGCCCAGCGCGAGGCGCAGGAGCAGCGCTTTCGGGTGCAAAGCCAGCAACAACTGCTGCGCGCCTACGAAAATTTCACCG
CCGCCCTCACCCACGATCTCAAGACACCGCTACAGGGTATGGCCCAGACGCTGCGCTTTTTTGCCCAGGGGCAATTCGGC
CGGCTCACCGACGCCCAGCGCCAGGTGGTGCAGCTATTCGAGCGCTCGCTCGGGACGCTTTTGGAATTGACCGATACGCT
GCTGACTATCTACCGCAACGAGCGGGAGCCCCTGGGGCTGAGGTGCCGCAGCGCCGACCTCGATGCCCTCGCGGCGGAGG
TGGTCACCCAGTGGATCGACCCGGCGGGGGCACGCCAGGTGACATTGGTTTACGAAGGGCTCGAAGGGGCGCAGGTGGAG
GCGGACCCGCTGCAGCTCGGCCGGGCGATCACCAATCTGGTGGCCAACGCCGTGCGTTACACCCGCCTGGGTTCCACCGT
GCAGGTGCGTGTGGAAAGCCGTACTGCCGAATATCAAATTCAGGTGAGCGACTGCGGCCCCGGCATTCCCGAGGGTGATC
TCGATCGGATCTTCGAGCAATTCTACCAATCGCCCCTGACGCGCCAGAGCCTGGGGACGGGACTGGGGCTCTATCTGAGC
CGGCAGATCGTCGAAGCCCACAGCGGGCGCATCTGGGCGGTCAACAACCGCGACGAAGGCTGCACTTTTGTGATGGCGTT
ACCTAAGACCGACCAAGGCCATGACTAG

Upstream 100 bases:

>100_bases
GACGAACCATGTCATCCTTTATGGGGAAATTCTAGAGGCTCCATATATATGAATTCATATAGATGCCACGGCCAACGGTA
GTGAGGGCGAGCATTCGGCC

Downstream 100 bases:

>100_bases
AAAAACCCGGGTACTGCTCATCGAGGACGACCCGGTGTTCCGGGTGGGCCTGCGGGTCATTTTTGAGCAAAGCGGGGTAG
CCGAACTGGTTGGGGAGGCT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MYTAFKLQEPRRRALYGIVALILFVETVEYFAPTGYIFSYLYAVPILIAANHFSERQTQWITFLSLVSTILGLFVPTFEL
NSLTLVNRLIICSALWTVGTLSLRYRQAQREAQEQRFRVQSQQQLLRAYENFTAALTHDLKTPLQGMAQTLRFFAQGQFG
RLTDAQRQVVQLFERSLGTLLELTDTLLTIYRNEREPLGLRCRSADLDALAAEVVTQWIDPAGARQVTLVYEGLEGAQVE
ADPLQLGRAITNLVANAVRYTRLGSTVQVRVESRTAEYQIQVSDCGPGIPEGDLDRIFEQFYQSPLTRQSLGTGLGLYLS
RQIVEAHSGRIWAVNNRDEGCTFVMALPKTDQGHD

Sequences:

>Translated_355_residues
MYTAFKLQEPRRRALYGIVALILFVETVEYFAPTGYIFSYLYAVPILIAANHFSERQTQWITFLSLVSTILGLFVPTFEL
NSLTLVNRLIICSALWTVGTLSLRYRQAQREAQEQRFRVQSQQQLLRAYENFTAALTHDLKTPLQGMAQTLRFFAQGQFG
RLTDAQRQVVQLFERSLGTLLELTDTLLTIYRNEREPLGLRCRSADLDALAAEVVTQWIDPAGARQVTLVYEGLEGAQVE
ADPLQLGRAITNLVANAVRYTRLGSTVQVRVESRTAEYQIQVSDCGPGIPEGDLDRIFEQFYQSPLTRQSLGTGLGLYLS
RQIVEAHSGRIWAVNNRDEGCTFVMALPKTDQGHD
>Mature_355_residues
MYTAFKLQEPRRRALYGIVALILFVETVEYFAPTGYIFSYLYAVPILIAANHFSERQTQWITFLSLVSTILGLFVPTFEL
NSLTLVNRLIICSALWTVGTLSLRYRQAQREAQEQRFRVQSQQQLLRAYENFTAALTHDLKTPLQGMAQTLRFFAQGQFG
RLTDAQRQVVQLFERSLGTLLELTDTLLTIYRNEREPLGLRCRSADLDALAAEVVTQWIDPAGARQVTLVYEGLEGAQVE
ADPLQLGRAITNLVANAVRYTRLGSTVQVRVESRTAEYQIQVSDCGPGIPEGDLDRIFEQFYQSPLTRQSLGTGLGLYLS
RQIVEAHSGRIWAVNNRDEGCTFVMALPKTDQGHD

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=256, Percent_Identity=30.859375, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790436, Length=272, Percent_Identity=27.2058823529412, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=28.5067873303167, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786783, Length=224, Percent_Identity=29.9107142857143, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=29.5833333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=28.2608695652174, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=25.6410256410256, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=27.9816513761468, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=21.9298245614035, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788279, Length=205, Percent_Identity=23.4146341463415, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790861, Length=217, Percent_Identity=28.110599078341, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40109; Mature: 40109

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTAFKLQEPRRRALYGIVALILFVETVEYFAPTGYIFSYLYAVPILIAANHFSERQTQW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITFLSLVSTILGLFVPTFELNSLTLVNRLIICSALWTVGTLSLRYRQAQREAQEQRFRVQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQQQLLRAYENFTAALTHDLKTPLQGMAQTLRFFAQGQFGRLTDAQRQVVQLFERSLGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LELTDTLLTIYRNEREPLGLRCRSADLDALAAEVVTQWIDPAGARQVTLVYEGLEGAQVE
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
ADPLQLGRAITNLVANAVRYTRLGSTVQVRVESRTAEYQIQVSDCGPGIPEGDLDRIFEQ
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHH
FYQSPLTRQSLGTGLGLYLSRQIVEAHSGRIWAVNNRDEGCTFVMALPKTDQGHD
HHHCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MYTAFKLQEPRRRALYGIVALILFVETVEYFAPTGYIFSYLYAVPILIAANHFSERQTQW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITFLSLVSTILGLFVPTFELNSLTLVNRLIICSALWTVGTLSLRYRQAQREAQEQRFRVQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQQQLLRAYENFTAALTHDLKTPLQGMAQTLRFFAQGQFGRLTDAQRQVVQLFERSLGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LELTDTLLTIYRNEREPLGLRCRSADLDALAAEVVTQWIDPAGARQVTLVYEGLEGAQVE
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
ADPLQLGRAITNLVANAVRYTRLGSTVQVRVESRTAEYQIQVSDCGPGIPEGDLDRIFEQ
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHH
FYQSPLTRQSLGTGLGLYLSRQIVEAHSGRIWAVNNRDEGCTFVMALPKTDQGHD
HHHCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA