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The map label for this gene is trpA

Identifier: 34498216

GI number: 34498216

Start: 2997203

End: 2997991

Strand: Reverse

Name: trpA

Synonym: CV_2761

Alternate gene names: 34498216

Gene position: 2997991-2997203 (Counterclockwise)

Preceding gene: 161594051

Following gene: 34498215

Centisome position: 63.1

GC content: 65.02

Gene sequence:

>789_bases
ATGTCACGCATTGCTAACTGTTTTGCCGAGCTCGCGGGCAAGAAAGCCCTGATCCCGTTCATCACCGCCGGCGATCCGCA
TCCTGGCTTGACCGTGTCGCTGATGCACGGCCTGGTGGACGGCGGCGCCGACATCATCGAGTTGGGCGTGCCGTTTTCCG
ACCCGATGGCCGACGGCCCGGTGATCCAGCGCGCGTCCGAGCGCGCGCTCAAGCACAAGGTCGGTCTGCGCCACGTGCTG
GAGATGGTGGCCGAGTTCCGTCGCGACAACGCAACGACGCCCGTGGTGCTGATGGGTTACCTGAATCCGCTGTGCGCGAT
GGGCTATACCGAATTTGCCAGCCGCGCCAAGGCGGCCGGCGTGGATGGCGTGCTGACGGTGGATTGTCCGCCGGAAGAGG
CCGCCGAGCTTGAGTCCGCGCTGGACGCCCACGGCCTGGACACCGTGTTCCTGGTGGCGCCGACCACGCCGCCGTCGCGG
GTGGCGGAAATCGCCAAATTGGCGCGCGGATACGTCTATTATGTGTCGCTGAAAGGCGTGACCGGCGCCGGACATCTGGA
CATTGAGGATGTAGCGCGTAAAATTGCTGCCCTCAGACAACAATTGCCGCTGCCGATAGGCGTGGGTTTCGGGATTCGCG
ACGCCGCGACTGCCAAGGCCATCGCGGCCGCCGCCGACGCGGTGGTGGTGGGCAGCAGGCTGGTGCAGGAAATTGAGGCG
GCGACACCCGAAACTGCCAGAGAGCAGTTAACTCGTCTGGTGGCCGAATTGAAGGCCGCCATCCGCTAG

Upstream 100 bases:

>100_bases
GCAAGGATCAGGTGATCCTGGTCAATCTGTCCGGCCGCGGCGACAAGGACATCAATACCGTTGCCGGCTTGGCCGGCATC
ACGCTGTGAAGGGGGCGAGA

Downstream 100 bases:

>100_bases
AACACAGACGGGGGCGCAGCGCCCCCGTCGCAATGTGGCCGGAATGATATCCGGCCACCCAGATTCAAGGAGTCAGCATG
AGCTGGTTGAACAAGCTCCT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSRIANCFAELAGKKALIPFITAGDPHPGLTVSLMHGLVDGGADIIELGVPFSDPMADGPVIQRASERALKHKVGLRHVL
EMVAEFRRDNATTPVVLMGYLNPLCAMGYTEFASRAKAAGVDGVLTVDCPPEEAAELESALDAHGLDTVFLVAPTTPPSR
VAEIAKLARGYVYYVSLKGVTGAGHLDIEDVARKIAALRQQLPLPIGVGFGIRDAATAKAIAAAADAVVVGSRLVQEIEA
ATPETAREQLTRLVAELKAAIR

Sequences:

>Translated_262_residues
MSRIANCFAELAGKKALIPFITAGDPHPGLTVSLMHGLVDGGADIIELGVPFSDPMADGPVIQRASERALKHKVGLRHVL
EMVAEFRRDNATTPVVLMGYLNPLCAMGYTEFASRAKAAGVDGVLTVDCPPEEAAELESALDAHGLDTVFLVAPTTPPSR
VAEIAKLARGYVYYVSLKGVTGAGHLDIEDVARKIAALRQQLPLPIGVGFGIRDAATAKAIAAAADAVVVGSRLVQEIEA
ATPETAREQLTRLVAELKAAIR
>Mature_261_residues
SRIANCFAELAGKKALIPFITAGDPHPGLTVSLMHGLVDGGADIIELGVPFSDPMADGPVIQRASERALKHKVGLRHVLE
MVAEFRRDNATTPVVLMGYLNPLCAMGYTEFASRAKAAGVDGVLTVDCPPEEAAELESALDAHGLDTVFLVAPTTPPSRV
AEIAKLARGYVYYVSLKGVTGAGHLDIEDVARKIAALRQQLPLPIGVGFGIRDAATAKAIAAAADAVVVGSRLVQEIEAA
TPETAREQLTRLVAELKAAIR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=36.3295880149813, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321412, Length=228, Percent_Identity=40.3508771929825, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CHRVO (Q7NUD9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902431.1
- ProteinModelPortal:   Q7NUD9
- SMR:   Q7NUD9
- GeneID:   2547672
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2761
- NMPDR:   fig|243365.1.peg.2761
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q7NUD9
- BioCyc:   CVIO243365:CV_2761-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27536; Mature: 27405

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIANCFAELAGKKALIPFITAGDPHPGLTVSLMHGLVDGGADIIELGVPFSDPMADGP
CCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCH
VIQRASERALKHKVGLRHVLEMVAEFRRDNATTPVVLMGYLNPLCAMGYTEFASRAKAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
VDGVLTVDCPPEEAAELESALDAHGLDTVFLVAPTTPPSRVAEIAKLARGYVYYVSLKGV
CCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECC
TGAGHLDIEDVARKIAALRQQLPLPIGVGFGIRDAATAKAIAAAADAVVVGSRLVQEIEA
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATPETAREQLTRLVAELKAAIR
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIANCFAELAGKKALIPFITAGDPHPGLTVSLMHGLVDGGADIIELGVPFSDPMADGP
CHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCH
VIQRASERALKHKVGLRHVLEMVAEFRRDNATTPVVLMGYLNPLCAMGYTEFASRAKAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
VDGVLTVDCPPEEAAELESALDAHGLDTVFLVAPTTPPSRVAEIAKLARGYVYYVSLKGV
CCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECC
TGAGHLDIEDVARKIAALRQQLPLPIGVGFGIRDAATAKAIAAAADAVVVGSRLVQEIEA
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATPETAREQLTRLVAELKAAIR
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782