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The map label for this gene is entD [H]

Identifier: 34498105

GI number: 34498105

Start: 2863663

End: 2864376

Strand: Reverse

Name: entD [H]

Synonym: CV_2650

Alternate gene names: 34498105

Gene position: 2864376-2863663 (Counterclockwise)

Preceding gene: 34498107

Following gene: 34498101

Centisome position: 60.29

GC content: 67.23

Gene sequence:

>714_bases
ATGCCCATTCCCGGCGCCGAATTAAGCGCTTTCCTGCACGATGTGTCTTATCTGTCTCTGGCGGAGGCGCCCGGCATGGC
CGCCATGCATTGCCGGTTCCGCATCGCGGCCTACGACGACGCCTGGTTCGGCGCTTTGGGCGTGGCGCGTCCGGATACGT
TGGCAAGGGCGGTGCCCAAGCGCAAGGCGGAATACCTGGCCGCGCGCTACCTGTGCCAGCTGCTGCTGGCCGAACGCGGC
CTGCCCACCGCGGTGGGCAGCGGCCAGCATCGGCAGCCGCTGTGGCCGGAGGGCTGGACGGGGACGATCACCCATAGCGA
CGAAACGGCGGTGGTGGCCTTGGCCCCCAGCGGCGAGGGCTTGATGCTGGGGCTGGATCTGGAGCGCTGGATGTCGGACA
AGACCGCCGACAACGTGCATGAGGGAATACTGGTGCCAGGAGAGCGCGAGGCGCTGGCCGGACCGTGGCCGTTTCCGCAT
GCGCTGACGCTGGCGTTTTCCGCCAAGGAGAGCCTGTTCAAGGCGCTGTACCCCAGAGTGGGGCAGTACTTCGACTTCAA
CGCCGCGGAAATGACGCGCGTGGATTGGGACGCCGGGCGTTTCAGCATCCGCCTGCTGAAGACGCTGGCGCCGGGGCTGG
AGGCCGGGCGGGCGTTCGACGGCAGTTTCCATCTGCGCGACGGTCAGGTGTTGACCCTGATCGCGGCGTTTTAA

Upstream 100 bases:

>100_bases
GGAACGCCGCCGGGCGCTTGCGTCCGCGCGGTGCGGTGGTTACCCTTGCAGGCTGCCTCGGAGGCGCCTTTTAATCATGC
AAACAGTTCATCTTGATACC

Downstream 100 bases:

>100_bases
GCTTGCCGGCTTTCACCGCCAGGGTTCGGCAATGGGGGCGCAGACATGCTCCCGCATCGATTGACTTTTGCGCGGGTGCC
GTCGCCTGGCCTTGGCAATG

Product: enterobactin synthetase component D /phosphopantetheinyl transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPIPGAELSAFLHDVSYLSLAEAPGMAAMHCRFRIAAYDDAWFGALGVARPDTLARAVPKRKAEYLAARYLCQLLLAERG
LPTAVGSGQHRQPLWPEGWTGTITHSDETAVVALAPSGEGLMLGLDLERWMSDKTADNVHEGILVPGEREALAGPWPFPH
ALTLAFSAKESLFKALYPRVGQYFDFNAAEMTRVDWDAGRFSIRLLKTLAPGLEAGRAFDGSFHLRDGQVLTLIAAF

Sequences:

>Translated_237_residues
MPIPGAELSAFLHDVSYLSLAEAPGMAAMHCRFRIAAYDDAWFGALGVARPDTLARAVPKRKAEYLAARYLCQLLLAERG
LPTAVGSGQHRQPLWPEGWTGTITHSDETAVVALAPSGEGLMLGLDLERWMSDKTADNVHEGILVPGEREALAGPWPFPH
ALTLAFSAKESLFKALYPRVGQYFDFNAAEMTRVDWDAGRFSIRLLKTLAPGLEAGRAFDGSFHLRDGQVLTLIAAF
>Mature_236_residues
PIPGAELSAFLHDVSYLSLAEAPGMAAMHCRFRIAAYDDAWFGALGVARPDTLARAVPKRKAEYLAARYLCQLLLAERGL
PTAVGSGQHRQPLWPEGWTGTITHSDETAVVALAPSGEGLMLGLDLERWMSDKTADNVHEGILVPGEREALAGPWPFPHA
LTLAFSAKESLFKALYPRVGQYFDFNAAEMTRVDWDAGRFSIRLLKTLAPGLEAGRAFDGSFHLRDGQVLTLIAAF

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=125, Percent_Identity=42.4, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 25836; Mature: 25705

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIPGAELSAFLHDVSYLSLAEAPGMAAMHCRFRIAAYDDAWFGALGVARPDTLARAVPK
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHCCCCCCCCHHHHHHHHH
RKAEYLAARYLCQLLLAERGLPTAVGSGQHRQPLWPEGWTGTITHSDETAVVALAPSGEG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCE
LMLGLDLERWMSDKTADNVHEGILVPGEREALAGPWPFPHALTLAFSAKESLFKALYPRV
EEEEECHHHHHCCCCCCHHHCCEECCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHH
GQYFDFNAAEMTRVDWDAGRFSIRLLKTLAPGLEAGRAFDGSFHLRDGQVLTLIAAF
CCCCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCEEEECCCCEEEEEEEC
>Mature Secondary Structure 
PIPGAELSAFLHDVSYLSLAEAPGMAAMHCRFRIAAYDDAWFGALGVARPDTLARAVPK
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHCCCCCCCCHHHHHHHHH
RKAEYLAARYLCQLLLAERGLPTAVGSGQHRQPLWPEGWTGTITHSDETAVVALAPSGEG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCE
LMLGLDLERWMSDKTADNVHEGILVPGEREALAGPWPFPHALTLAFSAKESLFKALYPRV
EEEEECHHHHHCCCCCCHHHCCEECCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHH
GQYFDFNAAEMTRVDWDAGRFSIRLLKTLAPGLEAGRAFDGSFHLRDGQVLTLIAAF
CCCCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHCCCCCCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]