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The map label for this gene is ispD

Identifier: 33863597

GI number: 33863597

Start: 1415835

End: 1416512

Strand: Reverse

Name: ispD

Synonym: PMT1330

Alternate gene names: 33863597

Gene position: 1416512-1415835 (Counterclockwise)

Preceding gene: 33863602

Following gene: 33863594

Centisome position: 58.76

GC content: 56.34

Gene sequence:

>678_bases
ATGCATCTGTTGATTGTCGCTGCAGGTAGCGGAAGCCGTATGGGAGCGGATCGCAACAAATTGCTTTTGCCTCTGGCAGG
ACGCCCTGTTCTTGCCTGGACGATTGATGCGGTGATGGAAGCCGATTCGATCACTTGGGTTGGCATTGTTGGCCAACCTG
TGGACCGAGCAATGATCATGGAGTTGTTGGCTGAGGCCGCCAAACCCGTCGTTTGGATTGAGGGGGGCAGCACAAGGCAG
GAATCCGTAGAGCGTGGATTACAGGCGTTGCCTTCAGTTGCGCAGCATGTTCTTATTCATGATGGGGCTAGATGTTTAGC
TGAAGCGGCTTTGATCAATCGCTGTGCTGAGGCGGTGGTGGCTGGCGCAGCGGTGATTGCGGCAACTCCGGTTACCGACA
CGATCAAGCGCGTTGATGGTCAAGGGATCATTACGGGCACACCAGATCGTGCGGAACTATGGGCAGCCCAGACTCCGCAG
GGCTTTGCTGTGGAGCAGCTCAAGCAAGGTCATGCTGAGGCTCAGGCCAAGGGTTGGACAGTGACTGACGACGCCTCGCT
TTATGAACGCCTGGGATGGCCGGTGCAGGTGCTGGAGGCCAGCCCTGCCAATATCAAAGTCACAACTCCGTTTGATCTCA
CCGTAGCCGAGGCAGTGATTGCACTGAGAGCAAACTAG

Upstream 100 bases:

>100_bases
ACCCGGGCTGAGGGCGAGAACACGCATCGATCCAGAGGCAGAGGCCTTGCGAGCTGATTGTATGGAGGAGGTTTCAGCCT
GCTTGCGGCAGGAGGCGCAT

Downstream 100 bases:

>100_bases
GAATCTGTTTGTGATCAATAGCCGATGCTTCAATATTGGATTGGCTGGCAATAATTTTGCTTTTATTGCCTTGAGTCAGA
TCAAAACTACAAAAACTTTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMEADSITWVGIVGQPVDRAMIMELLAEAAKPVVWIEGGSTRQ
ESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGAAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQ
GFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVIALRAN

Sequences:

>Translated_225_residues
MHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMEADSITWVGIVGQPVDRAMIMELLAEAAKPVVWIEGGSTRQ
ESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGAAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQ
GFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVIALRAN
>Mature_225_residues
MHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMEADSITWVGIVGQPVDRAMIMELLAEAAKPVVWIEGGSTRQ
ESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGAAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQ
GFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVIALRAN

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=234, Percent_Identity=25.2136752136752, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789104, Length=219, Percent_Identity=36.0730593607306, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PROMM (Q7V647)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895157.1
- ProteinModelPortal:   Q7V647
- STRING:   Q7V647
- GeneID:   1729082
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1330
- NMPDR:   fig|74547.1.peg.1324
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   PMAR74547:PMT1330-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23736; Mature: 23736

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMEADSITWVGIVGQPVDRAMIM
CEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHCCCCEEEEEEECCCHHHHHHH
ELLAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVV
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AGAAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQGFAVEQLKQGHAEAQAKGWT
HHHHHEEECCCHHHHHHCCCCEEEECCCCHHHEEECCCCCCCHHHHHHCCHHHHHCCCCE
VTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVIALRAN
ECCCHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHEECC
>Mature Secondary Structure
MHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMEADSITWVGIVGQPVDRAMIM
CEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHCCCCEEEEEEECCCHHHHHHH
ELLAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVV
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AGAAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQGFAVEQLKQGHAEAQAKGWT
HHHHHEEECCCHHHHHHCCCCEEEECCCCHHHEEECCCCCCCHHHHHHCCHHHHHCCCCE
VTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVIALRAN
ECCCHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642