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The map label for this gene is gatA [H]

Identifier: 33863565

GI number: 33863565

Start: 1384865

End: 1386643

Strand: Reverse

Name: gatA [H]

Synonym: PMT1297

Alternate gene names: 33863565

Gene position: 1386643-1384865 (Counterclockwise)

Preceding gene: 161350043

Following gene: 33863563

Centisome position: 57.52

GC content: 44.91

Gene sequence:

>1779_bases
GTGAATCATTCGAAGGGGTCGAAGATATTTAACCAAGGCATAAGGCTTGCGCTTGCTCTGAGCATGACTTGTCTTATGGC
TGAGAAGTCTATGGGCAACCAGTTTGGTAGTCAGAAATTTGGGATTAAGGAGTCTACAATTGAATCCATCCATGATGCTT
TTAAAGAGAGAGAGATTGATTGTGAGCAATTAATTCAGAGGTATTTATATCGGATAAAGAAGTATAATTTTAGCCTCGAA
AGAGGAGCTCCGCTTAATGCATTTGTTGCACTTAATCCGAATGCAGTCAGGCAGGCAAAGGCTCTGGATAGGCATTTTAA
ACAAAGCAATAAATTGATTGGCCCTTTGCATTGCATCCCGATTGCTGTTAAAGACAACATTGATACCGTTGACACTCCTT
CCACTTCTGGGTCGTTGGCTTTGCTTGGCTCTCAGCCAATCAGTAATGCTTTTTTGGTTAATAAATTACGAGCCGCTGGC
GGCATCATTATTGGTAAAGCCGCTATGGATGAATTTGCTTCTGGTGGGGAAGGAATTAGTGGTCGTAGCGGAAGGATTGG
CAATGCTTATGACCCTAATCAAAATTCCGGTGGCTCAAGTGGAGGCTCTGCGGTTGCAGTGAGTGCGAACTTTGCTGTGT
TAGGGATCGGTACGGATAATAGTGGCTCGGTCAGAGTGCCTGCAGTCTTCAACGGGGTTTATGCCATTCGTCCAAGCACG
GGGCTGATTAGTCATTCAGGAATTCTCCCGAGAGGCAACCTGGATGGAGTGGCAGGAGTGATGGCAAGGTCGATTCCTGA
TCTTGCTCTTGGCCTTGCTGCGATAGCAAGCACATCTGATCCTGATGATCATTTTACGAAACAAGTACCGCGCACAGATT
CCTATGCAAATAATTTAAAAACTGCTTCCCTTGATGGAATGCGAATTGGCGTGATTCGCAGTGTTGCAGGCAATGAGGTC
TTTGACGCCACCGACAAGACTTCAATGGCTCTATTCAATCAGGTCAAAGCCAGGCTTGAGAGAAAGGGTGCGTCACTTGT
TGAAATCAATCTGCCCTTATTCGACACGAACAGAGACAATAATATGGCGGGCGAGGCGGAAGATATTGATCAATACCTAG
GTTCTTTTGCTTCGACCAGAAGGAGCCTTCAGGACATATGTTTATCTGGTCGCACACGGCTAGGCGAGAAAGCCTGCATA
GGGTATATGGAAAGTATTGCGCCAAAGTACAGCGATCGATATGATTCTGCATTAAAGACTTTTGAGAAGAATAGAAACTA
TGTAGAGGGATTGATGCGAGAAGATGGCATTGATGCTTTGCTAATGCCACTTAGTTCTTGGCAACCCCCAAGCTATTACG
ATGATATGTATCGCACAGCCACAACGGAATCGCCTGTGGCATCAAACTCCGGATTGCCGGCAATTGCTTTAATTGCTGGT
TGGACATCGGCAACTCCAGCGATGCCGATCGGCTTTGAGTTGATTGGTTTTCAATATGGAGAGGGGGATTTGATCGGCCT
TGCGCAGGCTTATTCTTCTGGTCTTCCAGACAGGCCTTTGCCTGAATTGAACGCAGGTAATAATGATTCTCCTTTCGAGG
ATGTTTGTGTTCAAGGAATCAATTACTTTATTACTCAGGCTGGTTGGCATAGTTATAAGCAGTTCCTAAAGGATGCTAAT
GGTCAGAATATTGATCCAGTTGCTTATCAAAGGTTTTTTGAGAAGCAAACGCAAAAATTCGCTCAAGCAAATCAACAGTC
TGTCCAAGCTTGTGAATAA

Upstream 100 bases:

>100_bases
ATGTGCCGCTTCAATTTGAAAATTATCGATAATAAAACACTCTTGCGAATGTTGCTGTGTGTATAGTAGTGGCAGTAAAA
TTCTTGTTTAAGTATTAGAC

Downstream 100 bases:

>100_bases
CTGCTCTATTTATCCAGCTTTTTCCTGTTCTTGAGAGGCTTTTGCTCCGCAAACACAGGCAGAATTAGGGCTTGCTAAAA
AAACGAGAATTGAATTAACT

Product: hypothetical protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMGNQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNKLIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNKLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAVFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLALGLAAIASTSDPDDHFTKQVPRTDSYANNLKTASLDGMRIGVIRSVAGNEV
FDATDKTSMALFNQVKARLERKGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSATPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPDRPLPELNAGNNDSPFEDVCVQGINYFITQAGWHSYKQFLKDAN
GQNIDPVAYQRFFEKQTQKFAQANQQSVQACE

Sequences:

>Translated_592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMGNQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNKLIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNKLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAVFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLALGLAAIASTSDPDDHFTKQVPRTDSYANNLKTASLDGMRIGVIRSVAGNEV
FDATDKTSMALFNQVKARLERKGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSATPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPDRPLPELNAGNNDSPFEDVCVQGINYFITQAGWHSYKQFLKDAN
GQNIDPVAYQRFFEKQTQKFAQANQQSVQACE
>Mature_592_residues
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMGNQFGSQKFGIKESTIESIHDAFKEREIDCEQLIQRYLYRIKKYNFSLE
RGAPLNAFVALNPNAVRQAKALDRHFKQSNKLIGPLHCIPIAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNKLRAAG
GIIIGKAAMDEFASGGEGISGRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAVFNGVYAIRPST
GLISHSGILPRGNLDGVAGVMARSIPDLALGLAAIASTSDPDDHFTKQVPRTDSYANNLKTASLDGMRIGVIRSVAGNEV
FDATDKTSMALFNQVKARLERKGASLVEINLPLFDTNRDNNMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACI
GYMESIAPKYSDRYDSALKTFEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
WTSATPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPDRPLPELNAGNNDSPFEDVCVQGINYFITQAGWHSYKQFLKDAN
GQNIDPVAYQRFFEKQTQKFAQANQQSVQACE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=27.0428015564202, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI195972892, Length=234, Percent_Identity=29.9145299145299, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=412, Percent_Identity=26.4563106796117, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=24.8, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=223, Percent_Identity=26.457399103139, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=201, Percent_Identity=25.8706467661692, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=28.0623608017817, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=409, Percent_Identity=23.7163814180929, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=359, Percent_Identity=25.3481894150418, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=27.5488069414317, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652985, Length=475, Percent_Identity=25.6842105263158, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922090, Length=475, Percent_Identity=25.6842105263158, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24652981, Length=475, Percent_Identity=25.6842105263158, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24652983, Length=475, Percent_Identity=25.6842105263158, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=25.5623721881391, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356731, Length=243, Percent_Identity=28.8065843621399, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=24.8979591836735, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=24.8979591836735, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=24.8979591836735, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=27.9329608938547, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=27.9329608938547, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 63848; Mature: 63848

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMGNQFGSQKFGIKESTIESIHDAFKEREID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
CEQLIQRYLYRIKKYNFSLERGAPLNAFVALNPNAVRQAKALDRHFKQSNKLIGPLHCIP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEE
IAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNKLRAAGGIIIGKAAMDEFASGGEGIS
EEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCC
GRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAVFNGVYAIRPST
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCCEECCCEECCEEEECCCC
GLISHSGILPRGNLDGVAGVMARSIPDLALGLAAIASTSDPDDHFTKQVPRTDSYANNLK
CCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCC
TASLDGMRIGVIRSVAGNEVFDATDKTSMALFNQVKARLERKGASLVEINLPLFDTNRDN
CCCCCCEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCC
NMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACIGYMESIAPKYSDRYDSALKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
FEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
HHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
WTSATPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPDRPLPELNAGNNDSPFEDVCVQGI
CCCCCCCCCCCEEEEEEEECCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NYFITQAGWHSYKQFLKDANGQNIDPVAYQRFFEKQTQKFAQANQQSVQACE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHSKGSKIFNQGIRLALALSMTCLMAEKSMGNQFGSQKFGIKESTIESIHDAFKEREID
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
CEQLIQRYLYRIKKYNFSLERGAPLNAFVALNPNAVRQAKALDRHFKQSNKLIGPLHCIP
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEE
IAVKDNIDTVDTPSTSGSLALLGSQPISNAFLVNKLRAAGGIIIGKAAMDEFASGGEGIS
EEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCC
GRSGRIGNAYDPNQNSGGSSGGSAVAVSANFAVLGIGTDNSGSVRVPAVFNGVYAIRPST
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCCEECCCEECCEEEECCCC
GLISHSGILPRGNLDGVAGVMARSIPDLALGLAAIASTSDPDDHFTKQVPRTDSYANNLK
CCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCC
TASLDGMRIGVIRSVAGNEVFDATDKTSMALFNQVKARLERKGASLVEINLPLFDTNRDN
CCCCCCEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCC
NMAGEAEDIDQYLGSFASTRRSLQDICLSGRTRLGEKACIGYMESIAPKYSDRYDSALKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
FEKNRNYVEGLMREDGIDALLMPLSSWQPPSYYDDMYRTATTESPVASNSGLPAIALIAG
HHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
WTSATPAMPIGFELIGFQYGEGDLIGLAQAYSSGLPDRPLPELNAGNNDSPFEDVCVQGI
CCCCCCCCCCCEEEEEEEECCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
NYFITQAGWHSYKQFLKDANGQNIDPVAYQRFFEKQTQKFAQANQQSVQACE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA