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The map label for this gene is glcD [H]

Identifier: 33863563

GI number: 33863563

Start: 1381752

End: 1383260

Strand: Reverse

Name: glcD [H]

Synonym: PMT1295

Alternate gene names: 33863563

Gene position: 1383260-1381752 (Counterclockwise)

Preceding gene: 33863565

Following gene: 33863560

Centisome position: 57.38

GC content: 55.67

Gene sequence:

>1509_bases
TTGCTGCCTAGGTTGTTGGACAGATGCGCGGGCATGGTGTCCCACGACTGGACTGCTCTAGACAGGGACTTAAGGCGATT
TTTGCCGAAGAAGTCGGTGGTCTCCAAGCGGCAAGAGTTGCTCGTTTACGACTGTGACGGGTTAACCATCGATCGTCATT
GTCCTCCTTTGGCAGTGTTGCCCGAGACCACTGAGGAGGTGTCTCAGATCTTGGCTTGTTGTCATCGCCATAGCATCCCT
TTTGTCGCTAGGGGTAGTGGTACGGGGCTCTCGGGAGGTGCATTAGTGGAGCAAGAGGCGCTGTTGGTGGTGACCAGTCG
CATGCGCAGGATCCTTTCGATGGACCTTGATAACCACACCATTACGGTGCAGCCAGGAGTGATCAATAGTTGGGTGACTC
GGGCGGTCGCTGGGGAAGGGTTTTATTACGCCCCTGATCCTTCCAGTCAGGTGGTGTGCAGCATCGGTGGCAATGTGGCC
GAGAACTCCGGTGGTGTGCACTGTCTCAAATATGGCGTCACCAGTAATCATGTTCTGGGGCTTGAAGTAGTGCTTGCTGA
TGGAACGGTGACCCTTCTCGGTGGTGGACTGCCGGAGCATGCGGAGCTCGACCTGCGTGGGGCCTTTATTGGCAGCGAAG
GCACCCTGGGGATCGCCACAGCGATCACTCTGCGGCTCTTGCGGGCGCCGCAACATGTGACTGTGCTGCTTGCAGATTTC
CCCACCATGGAGTCTGCTGGCGAGGCGGTCTGCCAGGTCACCGCTGCCGGAGTTTTACCTGCGGGTATGGAGATCATGGA
CAACTTCATGATTAATGCAGTCAATGATCTGTTTGGCTTCGACGAATACCCTCGTGATGCCGCGGCTGTGCTGCTGATCG
AGCTCGATGGTCAGGCGGCGGAAGTCACTGTGGCCGCAGACCAGGCCAGTGGACTTTGTAGCAAGGCAGGAGCGAGGACC
ATTCGTCGTGCAGAAGAGCCTGCTGACCGTGCCTTGCTTTGGAAGGGACGCAAGTCAGCATTTGCTGCGGTTGGGCAGAT
TTCACCCACCTATTACGTGCAGGACGGTGTCGTGCCACGCAGTACTTTGCCGAGGGTGCTAATGGCAATAGAGAGGCTTA
GTCGGGAGTATGAGTTGCCGGTGGCCAATGTTTTTCATGCTGGCGATGGCAATTTGCATCCTTTAATCCTCTACAACGCA
GACACGCCTGATGTGGAGTCTCGTGTTCGGGATCTGGGGTCAGCCATTCTGCGTGAGTGTCTTGATGTTGGCGGCAGCAT
CACTGGTGAACATGGGGTTGGGGCTGATAAGCGCTGTTATCTCGATTGGATGTTTGCAGCGGATGATCTGTCCACAATGA
AACTGCTACGAAGAGCCTTCGATCCAGATGGACGGGCCAATCCTGACAAAATTTTCCCCACTCCAAAAAGCTGTGGTGAG
TCGGCTCGGCGCCAGGTCATCTTGACTTCAGAAGGTCTTGAGATGCCTTCAGAAGCCAAGGCATTCTGA

Upstream 100 bases:

>100_bases
AGGGACCGAAGCCAAGTGGCTGATACGAACCGTTCGCCATCTTGACCCAAAAAGGTCAGAAGCGGATGAACTGTGTCAGG
CCATCCGTTTACTTCAGGGT

Downstream 100 bases:

>100_bases
CTGCTCCGCAGATGAATCCTTGACTGCGACTATTGCCTTAAAGAATCATGTCCTTCAATTGCTGAACAGTTCCGACATCT
CCTCATCGTCATCAGTTGGA

Product: glycolate oxidase subunit glcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLPRLLDRCAGMVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHSIP
FVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVA
ENSGGVHCLKYGVTSNHVLGLEVVLADGTVTLLGGGLPEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADF
PTMESAGEAVCQVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQAAEVTVAADQASGLCSKAGART
IRRAEEPADRALLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLMAIERLSREYELPVANVFHAGDGNLHPLILYNA
DTPDVESRVRDLGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGE
SARRQVILTSEGLEMPSEAKAF

Sequences:

>Translated_502_residues
MLPRLLDRCAGMVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHSIP
FVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVA
ENSGGVHCLKYGVTSNHVLGLEVVLADGTVTLLGGGLPEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADF
PTMESAGEAVCQVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQAAEVTVAADQASGLCSKAGART
IRRAEEPADRALLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLMAIERLSREYELPVANVFHAGDGNLHPLILYNA
DTPDVESRVRDLGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGE
SARRQVILTSEGLEMPSEAKAF
>Mature_502_residues
MLPRLLDRCAGMVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVLPETTEEVSQILACCHRHSIP
FVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHTITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVA
ENSGGVHCLKYGVTSNHVLGLEVVLADGTVTLLGGGLPEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADF
PTMESAGEAVCQVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQAAEVTVAADQASGLCSKAGART
IRRAEEPADRALLWKGRKSAFAAVGQISPTYYVQDGVVPRSTLPRVLMAIERLSREYELPVANVFHAGDGNLHPLILYNA
DTPDVESRVRDLGSAILRECLDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGE
SARRQVILTSEGLEMPSEAKAF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=432, Percent_Identity=31.9444444444444, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI37595754, Length=454, Percent_Identity=30.3964757709251, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI119964728, Length=461, Percent_Identity=29.9349240780911, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI4501993, Length=452, Percent_Identity=21.2389380530973, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789351, Length=471, Percent_Identity=47.5583864118896, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=23.8095238095238, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI48994907, Length=446, Percent_Identity=23.542600896861, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71992373, Length=432, Percent_Identity=31.25, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71992381, Length=432, Percent_Identity=30.3240740740741, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=364, Percent_Identity=31.043956043956, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17534361, Length=426, Percent_Identity=25.8215962441315, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556096, Length=218, Percent_Identity=25.6880733944954, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=431, Percent_Identity=29.2343387470998, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320023, Length=461, Percent_Identity=26.4642082429501, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320764, Length=464, Percent_Identity=22.6293103448276, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18921117, Length=474, Percent_Identity=25.9493670886076, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24639277, Length=474, Percent_Identity=25.9493670886076, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24639275, Length=474, Percent_Identity=25.9493670886076, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653753, Length=455, Percent_Identity=22.6373626373626, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53973; Mature: 53973

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRLLDRCAGMVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVL
CCHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCEEECCCCCCEEEC
PETTEEVSQILACCHRHSIPFVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHT
CCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCE
ITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKYGVTSNHVLG
EEECCCHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEE
LEVVLADGTVTLLGGGLPEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADF
EEEEEECCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECC
PTMESAGEAVCQVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQAA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEE
EVTVAADQASGLCSKAGARTIRRAEEPADRALLWKGRKSAFAAVGQISPTYYVQDGVVPR
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEEECCCCCH
STLPRVLMAIERLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRDLGSAILREC
HHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGE
HHCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
SARRQVILTSEGLEMPSEAKAF
HHHCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLPRLLDRCAGMVSHDWTALDRDLRRFLPKKSVVSKRQELLVYDCDGLTIDRHCPPLAVL
CCHHHHHHHCCHHHCCHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCEEECCCCCCEEEC
PETTEEVSQILACCHRHSIPFVARGSGTGLSGGALVEQEALLVVTSRMRRILSMDLDNHT
CCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCE
ITVQPGVINSWVTRAVAGEGFYYAPDPSSQVVCSIGGNVAENSGGVHCLKYGVTSNHVLG
EEECCCHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEE
LEVVLADGTVTLLGGGLPEHAELDLRGAFIGSEGTLGIATAITLRLLRAPQHVTVLLADF
EEEEEECCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECC
PTMESAGEAVCQVTAAGVLPAGMEIMDNFMINAVNDLFGFDEYPRDAAAVLLIELDGQAA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEE
EVTVAADQASGLCSKAGARTIRRAEEPADRALLWKGRKSAFAAVGQISPTYYVQDGVVPR
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEEECCCCCH
STLPRVLMAIERLSREYELPVANVFHAGDGNLHPLILYNADTPDVESRVRDLGSAILREC
HHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LDVGGSITGEHGVGADKRCYLDWMFAADDLSTMKLLRRAFDPDGRANPDKIFPTPKSCGE
HHCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
SARRQVILTSEGLEMPSEAKAF
HHHCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]