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The map label for this gene is cbiJ [H]

Identifier: 33863532

GI number: 33863532

Start: 1353395

End: 1354216

Strand: Reverse

Name: cbiJ [H]

Synonym: PMT1264

Alternate gene names: 33863532

Gene position: 1354216-1353395 (Counterclockwise)

Preceding gene: 33863534

Following gene: 33863531

Centisome position: 56.17

GC content: 58.64

Gene sequence:

>822_bases
ATGCAAGCTTGCAGGAATGACCAGCCGCATGTCTGGTTGCTATCTGGGACTGGTGAAGGACCTGTTTTGGTGGCCGCTTT
GGTGGCTCAGGGATGGCGAGTCAGTGTCAGTGTCGTCAGTGCTAGCGCCGCAGGGGCCTACGCAGGTCTGTCCTTGGAAG
ATCTTTGGACGGGCCCCCTCGACGGAGTGGAAGGGATACTCGGGGTGCTTGATCAGGCTCGTCATCGCCATCAAGGTTTT
GATTGGGTGGTGGATGTGACCCATCCCTTTGCAGTCAAGATCAGTACGGATCTGCAGCGTGCCTGTCAGGAATTCGCTCA
GCCTTTGTTGCGCTTTGAGCGTCCCATGGAGGGTTGTGGAGAAGCATCCCTGATCGCGAGCTCGGCTGATTTGGCAAGGC
AAACATTGCAAGGAAGTCGATTGTTGATGGCTCTTGGGGCAAGGCATCTTGGCCAGGCTGTCAGGGCAGCTCGTCAGGCT
GGAGCTCAAGTGTTTGCCCGTGTGCTTCCTTCGCCGGAAAGTCTTCGACATGCTTTGGCGAGTGGCTTGCCCGAGCACCA
TCTGGCTGTTGTGCGTCCGCTGCAGGGGCAATCTCATGGCGAACTGGAGTCAGCCTTGTGCCGGCGCTGGTCGATTACAG
CAGTGGTTTGCAGGCAGTCGGGGGGGGTGACGGAGCAGCTCTGGCACAAGATCTGTCGGCAGCAGGGGCTTGGCCTTTGG
TTGATCTCCCGGCCACAGCCCTGCGGTGCAGTAGAAGCCATCGGTAGCGTTGAGAGCCTGCTCAATCGGCTCTCAACCGA
TCGAGTAGCTCCACTGTGCTGA

Upstream 100 bases:

>100_bases
GTTTGGTTGTCTTGGGTATAACGAATGGTTGGTGCCTGGATCACTTCCACTTCAAGTACGCAGTGGTTCATGTCCTTCCC
TTGGAGTGAGAGTCATTTTG

Downstream 100 bases:

>100_bases
CGTTCTTTGCAAAGGTCTTCATGGTTGATCGAGGATTCTCATCGACACTGGTTTTGGTGTTGACCACAGAGGCAAACGCA
AACCTGGCCGAGGGATTGGC

Product: precorrin-6x reductase CbiJ/CobK

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQACRNDQPHVWLLSGTGEGPVLVAALVAQGWRVSVSVVSASAAGAYAGLSLEDLWTGPLDGVEGILGVLDQARHRHQGF
DWVVDVTHPFAVKISTDLQRACQEFAQPLLRFERPMEGCGEASLIASSADLARQTLQGSRLLMALGARHLGQAVRAARQA
GAQVFARVLPSPESLRHALASGLPEHHLAVVRPLQGQSHGELESALCRRWSITAVVCRQSGGVTEQLWHKICRQQGLGLW
LISRPQPCGAVEAIGSVESLLNRLSTDRVAPLC

Sequences:

>Translated_273_residues
MQACRNDQPHVWLLSGTGEGPVLVAALVAQGWRVSVSVVSASAAGAYAGLSLEDLWTGPLDGVEGILGVLDQARHRHQGF
DWVVDVTHPFAVKISTDLQRACQEFAQPLLRFERPMEGCGEASLIASSADLARQTLQGSRLLMALGARHLGQAVRAARQA
GAQVFARVLPSPESLRHALASGLPEHHLAVVRPLQGQSHGELESALCRRWSITAVVCRQSGGVTEQLWHKICRQQGLGLW
LISRPQPCGAVEAIGSVESLLNRLSTDRVAPLC
>Mature_273_residues
MQACRNDQPHVWLLSGTGEGPVLVAALVAQGWRVSVSVVSASAAGAYAGLSLEDLWTGPLDGVEGILGVLDQARHRHQGF
DWVVDVTHPFAVKISTDLQRACQEFAQPLLRFERPMEGCGEASLIASSADLARQTLQGSRLLMALGARHLGQAVRAARQA
GAQVFARVLPSPESLRHALASGLPEHHLAVVRPLQGQSHGELESALCRRWSITAVVCRQSGGVTEQLWHKICRQQGLGLW
LISRPQPCGAVEAIGSVESLLNRLSTDRVAPLC

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 29298; Mature: 29298

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQACRNDQPHVWLLSGTGEGPVLVAALVAQGWRVSVSVVSASAAGAYAGLSLEDLWTGPL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHCCCCHHHHCCCCC
DGVEGILGVLDQARHRHQGFDWVVDVTHPFAVKISTDLQRACQEFAQPLLRFERPMEGCG
HHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCC
EASLIASSADLARQTLQGSRLLMALGARHLGQAVRAARQAGAQVFARVLPSPESLRHALA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SGLPEHHLAVVRPLQGQSHGELESALCRRWSITAVVCRQSGGVTEQLWHKICRQQGLGLW
HCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEE
LISRPQPCGAVEAIGSVESLLNRLSTDRVAPLC
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQACRNDQPHVWLLSGTGEGPVLVAALVAQGWRVSVSVVSASAAGAYAGLSLEDLWTGPL
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHCCCCHHHHCCCCC
DGVEGILGVLDQARHRHQGFDWVVDVTHPFAVKISTDLQRACQEFAQPLLRFERPMEGCG
HHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCC
EASLIASSADLARQTLQGSRLLMALGARHLGQAVRAARQAGAQVFARVLPSPESLRHALA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SGLPEHHLAVVRPLQGQSHGELESALCRRWSITAVVCRQSGGVTEQLWHKICRQQGLGLW
HCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEE
LISRPQPCGAVEAIGSVESLLNRLSTDRVAPLC
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]