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The map label for this gene is dnaA

Identifier: 33863457

GI number: 33863457

Start: 1277265

End: 1278662

Strand: Reverse

Name: dnaA

Synonym: PMT1187

Alternate gene names: 33863457

Gene position: 1278662-1277265 (Counterclockwise)

Preceding gene: 33863461

Following gene: 33863456

Centisome position: 53.04

GC content: 51.5

Gene sequence:

>1398_bases
GTGGTGATAACGGGCAATGAGCTGTGGTCCAAGGTGCAGCAGGCCCTTCAGCACAATCTCAGCAAACCTACGTTTGAGAC
ATGGATTCGTCCTGCCATTTGTAGTGGTTTTCGGGACGGGGAGTTGACTCTGATTGCTCCAAATAGCTTTGCAAGTAACT
GGCTTCGCAAGAATTACGTTCAGACAATCGAGGCGGCTGCGGCCAAGATTTACGGCCAGTCAGTGAGGGTGTCTGTTCAG
GCTCAGGAGGAAGACTCTGCTGAGAGGGTGTTACCACCAATGACATCGGCTTCCATCCCTTCACCTTTGCCTACTGCAGA
AACAACCACTGCCTCTGTAGCCCCTTCTAGTGGCCCTCGGCGGATTCTTCCAGGCCTGAACCTGCGCTATGTCTTCAATC
GGTTCGTCGTGGGGCCGAACAGTCGCATGGCCCATGCTGCTGCGCTAGCCGTTGCAGAAGCTCCTGGGCGAGAGTTCAAT
CCTTTATTCATTTGCGGCGGTGTTGGCCTAGGCAAGACTCATCTGATGCAGGCTATTGGTCACTACAGGCTTGAGATCAA
TCCCGAAGCAAAGGTGGCTTATGTTTCTACAGAGACCTTCACGAATGACTTAATTCAAGCTATTCGGAAGGATGGTATGC
AAGCTTTCCGAGATCGATATCGTGCCACTGATTTGATTTTGGTTGACGATATTCAGTTTATTGAAGGCAAGGAATACACC
CAGGAAGAGTTCTTTCATACCTTTAATGCTCTCCATGAAGCAGGTAGGCAGATTGTGATCGCTAGTGATAGACCTCCAAG
TCAGATTCCAAAGCTTCAAGAACGCCTAATCTCTCGATTCTCTATGGGCTTGATCGCTGATATTCAGTCGCCTGACCTTG
AGACTCGCATGGCAATTTTGCAGAAGAAGGCGGAACAGGAACGCATGATGCTGCCTCGCGATTTAATTCAGTACATCGCT
GGGCGCTTCACCTCCAATATCCGTGAACTTGAGGGGGCTTTGACCAGGGCAGTTGCCTTTGCTTCGATCACGGGGCTGCC
GATGACAGTTGAATCGGTAGCGCCAATGCTTGACCCTAATGGTCAAGGTGTGGACGTTACCCCACAACAAGTGATTGATA
AGGTCTCTGAGGTTTTTGACGTGACTCCTCAAGACATGCGTAGTAGCAGTCGAAGGCGGGCCGTTAGCCATGCTCGGCAG
GTGGGCATGTACCTGATGCGTCAGGGCACGGATTTGAGCTTGCCTCGCATCGGTGAGACTTTTGGGGGAAAGGACCACAC
CACAGTGATGTATGCCATCGAGCAAGTGGAGAAAAGGTTGTCCAGTGATCCTCAGTTGGCTAGTCAGGTTCGGCGTGTGA
GAGATCTGCTGCAGATCGATTCACGCCGTCGGCGATAG

Upstream 100 bases:

>100_bases
TGACAAACGCAGGTGCCAGGGAACGAGAACATCAGGGTCTTATTAAAGACGGCTTGCTCGATAGAAGTTGCGTAGATTCA
GCCATCGCAGAGGAAAGGAT

Downstream 100 bases:

>100_bases
GTTTCAACAATTTGATTATTTGACAAGATTAGATGAGGTGTATGAGATTTTGTTTGCATCAACATCAGCCTTGCAATGCC
TTTCCCTCAAAACGTTCCAG

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVITGNELWSKVQQALQHNLSKPTFETWIRPAICSGFRDGELTLIAPNSFASNWLRKNYVQTIEAAAAKIYGQSVRVSVQ
AQEEDSAERVLPPMTSASIPSPLPTAETTTASVAPSSGPRRILPGLNLRYVFNRFVVGPNSRMAHAAALAVAEAPGREFN
PLFICGGVGLGKTHLMQAIGHYRLEINPEAKVAYVSTETFTNDLIQAIRKDGMQAFRDRYRATDLILVDDIQFIEGKEYT
QEEFFHTFNALHEAGRQIVIASDRPPSQIPKLQERLISRFSMGLIADIQSPDLETRMAILQKKAEQERMMLPRDLIQYIA
GRFTSNIRELEGALTRAVAFASITGLPMTVESVAPMLDPNGQGVDVTPQQVIDKVSEVFDVTPQDMRSSSRRRAVSHARQ
VGMYLMRQGTDLSLPRIGETFGGKDHTTVMYAIEQVEKRLSSDPQLASQVRRVRDLLQIDSRRRR

Sequences:

>Translated_465_residues
MVITGNELWSKVQQALQHNLSKPTFETWIRPAICSGFRDGELTLIAPNSFASNWLRKNYVQTIEAAAAKIYGQSVRVSVQ
AQEEDSAERVLPPMTSASIPSPLPTAETTTASVAPSSGPRRILPGLNLRYVFNRFVVGPNSRMAHAAALAVAEAPGREFN
PLFICGGVGLGKTHLMQAIGHYRLEINPEAKVAYVSTETFTNDLIQAIRKDGMQAFRDRYRATDLILVDDIQFIEGKEYT
QEEFFHTFNALHEAGRQIVIASDRPPSQIPKLQERLISRFSMGLIADIQSPDLETRMAILQKKAEQERMMLPRDLIQYIA
GRFTSNIRELEGALTRAVAFASITGLPMTVESVAPMLDPNGQGVDVTPQQVIDKVSEVFDVTPQDMRSSSRRRAVSHARQ
VGMYLMRQGTDLSLPRIGETFGGKDHTTVMYAIEQVEKRLSSDPQLASQVRRVRDLLQIDSRRRR
>Mature_465_residues
MVITGNELWSKVQQALQHNLSKPTFETWIRPAICSGFRDGELTLIAPNSFASNWLRKNYVQTIEAAAAKIYGQSVRVSVQ
AQEEDSAERVLPPMTSASIPSPLPTAETTTASVAPSSGPRRILPGLNLRYVFNRFVVGPNSRMAHAAALAVAEAPGREFN
PLFICGGVGLGKTHLMQAIGHYRLEINPEAKVAYVSTETFTNDLIQAIRKDGMQAFRDRYRATDLILVDDIQFIEGKEYT
QEEFFHTFNALHEAGRQIVIASDRPPSQIPKLQERLISRFSMGLIADIQSPDLETRMAILQKKAEQERMMLPRDLIQYIA
GRFTSNIRELEGALTRAVAFASITGLPMTVESVAPMLDPNGQGVDVTPQQVIDKVSEVFDVTPQDMRSSSRRRAVSHARQ
VGMYLMRQGTDLSLPRIGETFGGKDHTTVMYAIEQVEKRLSSDPQLASQVRRVRDLLQIDSRRRR

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=446, Percent_Identity=38.3408071748879, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_PROMM (Q7V6H4)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895017.1
- ProteinModelPortal:   Q7V6H4
- STRING:   Q7V6H4
- GeneID:   1729120
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1187
- NMPDR:   fig|74547.1.peg.1184
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   FNELHAN
- ProtClustDB:   PRK00149
- BioCyc:   PMAR74547:PMT1187-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51908; Mature: 51908

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITGNELWSKVQQALQHNLSKPTFETWIRPAICSGFRDGELTLIAPNSFASNWLRKNYV
CEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHH
QTIEAAAAKIYGQSVRVSVQAQEEDSAERVLPPMTSASIPSPLPTAETTTASVAPSSGPR
HHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RILPGLNLRYVFNRFVVGPNSRMAHAAALAVAEAPGREFNPLFICGGVGLGKTHLMQAIG
CCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHC
HYRLEINPEAKVAYVSTETFTNDLIQAIRKDGMQAFRDRYRATDLILVDDIQFIEGKEYT
CEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCHH
QEEFFHTFNALHEAGRQIVIASDRPPSQIPKLQERLISRFSMGLIADIQSPDLETRMAIL
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHH
QKKAEQERMMLPRDLIQYIAGRFTSNIRELEGALTRAVAFASITGLPMTVESVAPMLDPN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCC
GQGVDVTPQQVIDKVSEVFDVTPQDMRSSSRRRAVSHARQVGMYLMRQGTDLSLPRIGET
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
FGGKDHTTVMYAIEQVEKRLSSDPQLASQVRRVRDLLQIDSRRRR
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVITGNELWSKVQQALQHNLSKPTFETWIRPAICSGFRDGELTLIAPNSFASNWLRKNYV
CEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHH
QTIEAAAAKIYGQSVRVSVQAQEEDSAERVLPPMTSASIPSPLPTAETTTASVAPSSGPR
HHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RILPGLNLRYVFNRFVVGPNSRMAHAAALAVAEAPGREFNPLFICGGVGLGKTHLMQAIG
CCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHC
HYRLEINPEAKVAYVSTETFTNDLIQAIRKDGMQAFRDRYRATDLILVDDIQFIEGKEYT
CEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCHH
QEEFFHTFNALHEAGRQIVIASDRPPSQIPKLQERLISRFSMGLIADIQSPDLETRMAIL
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHH
QKKAEQERMMLPRDLIQYIAGRFTSNIRELEGALTRAVAFASITGLPMTVESVAPMLDPN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCC
GQGVDVTPQQVIDKVSEVFDVTPQDMRSSSRRRAVSHARQVGMYLMRQGTDLSLPRIGET
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
FGGKDHTTVMYAIEQVEKRLSSDPQLASQVRRVRDLLQIDSRRRR
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642