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The map label for this gene is F18O21.290 [H]

Identifier: 33863444

GI number: 33863444

Start: 1265520

End: 1266794

Strand: Reverse

Name: F18O21.290 [H]

Synonym: PMT1174

Alternate gene names: 33863444

Gene position: 1266794-1265520 (Counterclockwise)

Preceding gene: 33863447

Following gene: 109150046

Centisome position: 52.55

GC content: 56.0

Gene sequence:

>1275_bases
ATGGGATTCGCCCCAGTCAGCACCTGTCTCTTGAATGGCCCGCTCGGCAGTCTGGAAGTCAGTTGCAAACAAAGCGGCTT
GAACCAACCCACCACCCACTATCGCGAAGGCTCAGTCGTTTTGGAGCCAGGAGGTGGTTTCTTCCGCCCAGAGTCTCGTC
CTTCCCGTGATCTTTCGGTTTTATTGGCTGCTTTTCAGCAGCAGAGGAGAGAGAAGTTTTCGCCTGCTTTGCGCTGGTTG
GACCTTATGGCTGGTTGCGGTATTCGTGCTTTGCGCTGGGGGTTGGAGGCTGTTAAGCCCTCATTGTTGGCGTCAGGAGT
AGAGCAGCTGCCAGAGCTGTGGGTGAATGATGCCGATCTTGAGCGCGCTTCTCTTTTGGAGAGGAATCTGGCCCCTCTGC
GCCAAGAGCAAATCTCGCTAAAGCTTTTTCATCAGCCCGCTGAGGTCCTGCTGAGCCGTGCCTACCTCGATCAGTGTCGT
TTTGATCTGATTGATCTCGATTGTTTTGGTTGTCCGAATGCGTTTCTGCAACCTGTGTTGCAGGTTCTCGCTTCTGAGGG
GTTTTTGCTGCTTTCAAGCAGTGATGGTCGCTCTCCCACAGGTCATGACCGGCCAGGGGCAATTCGCAGTTTGGCTGCTG
CGGCTCGGGTGCATCCAGCCAGCTGGGAGATCGCTTTGAGGCTGCAGCTTGGGGTTCTTGCACGACATGCCTGGCTGCTA
GGGCGTGGCTTGGAGCCCCTGGTCAGCTTTAGTGAAGGTCGTACTTTTCGTGTTGCGGTAAGGATCAAGCGTCGAATTTC
TGCATGTGAGGAGAGCCATCTTGGGCTTTTAGCTCGCTGTGAGGCTTGTGGTGCTCAGCTTGCTCAACCACTTCTCTCCC
TGCAGGGATGGTCAGGGTGTATGTGTTCACAAGGAGGTGGCCGCTGGGCGGTGAGCGGTCCCCTTTGGCTGGGCCCCCTT
CAGTCGCCAGTTGTGTTGGCCGATTTGTTGGTGTTAGCAGAACAGTCGATCGTTCCGATCGCATCCGCCACACTTCGGCT
GTTGCATCGCTTGCAGTCTGATATCGGCTTGCCGGTCTATTGCTGGTCCACAGCTGAACTGGCCAAGCGTCTGGTTTTGT
CTGGCCCGCCGTCTGTTTTGGCATTGGTCAGGGCTTTGCGTTTTGCTGGCCATGAGGCTTGCGGCAGTGCCGTGATGGCA
GGACAACTACGAACCGATGCGACAGTGACCGAGCTTTTGCGGATTTGCCACGAGATGTGTCAGGAAGGGCGTTAA

Upstream 100 bases:

>100_bases
GAGCGCTGCTGGCACCGAATCCGTGGATGAGCACGAGTGGCCGTGCATCCTCTGCTCCCAGTACACGCCAGTGACAACGT
AGGCCTTGCCAATACCAATC

Downstream 100 bases:

>100_bases
ATGGTGTGCGTGATTCAAATGCCCATTCATGGCTTCGGAGATTTTCGGGATTGCTGCCGTCTTCTGGGTGTTGATCCCAG
TTGGTCTTGCAGGTGGTGCT

Product: N2,N2-dimethylguanosine tRNA methyltransferase-like protein

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFSPALRWL
DLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCR
FDLIDLDCFGCPNAFLQPVLQVLASEGFLLLSSSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
GRGLEPLVSFSEGRTFRVAVRIKRRISACEESHLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPL
QSPVVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLVLSGPPSVLALVRALRFAGHEACGSAVMA
GQLRTDATVTELLRICHEMCQEGR

Sequences:

>Translated_424_residues
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFSPALRWL
DLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCR
FDLIDLDCFGCPNAFLQPVLQVLASEGFLLLSSSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
GRGLEPLVSFSEGRTFRVAVRIKRRISACEESHLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPL
QSPVVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLVLSGPPSVLALVRALRFAGHEACGSAVMA
GQLRTDATVTELLRICHEMCQEGR
>Mature_423_residues
GFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSVLLAAFQQQRREKFSPALRWLD
LMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADLERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRF
DLIDLDCFGCPNAFLQPVLQVLASEGFLLLSSSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLLG
RGLEPLVSFSEGRTFRVAVRIKRRISACEESHLGLLARCEACGAQLAQPLLSLQGWSGCMCSQGGGRWAVSGPLWLGPLQ
SPVVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVYCWSTAELAKRLVLSGPPSVLALVRALRFAGHEACGSAVMAG
QLRTDATVTELLRICHEMCQEGR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [H]

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family [H]

Homologues:

Organism=Homo sapiens, GI8923219, Length=361, Percent_Identity=26.0387811634349, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI209862871, Length=361, Percent_Identity=26.0387811634349, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI216548445, Length=345, Percent_Identity=24.9275362318841, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320325, Length=302, Percent_Identity=24.1721854304636, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921190, Length=435, Percent_Identity=23.9080459770115, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022923
- InterPro:   IPR002905 [H]

Pfam domain/function: PF02005 TRM [H]

EC number: =2.1.1.32 [H]

Molecular weight: Translated: 46064; Mature: 45933

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSV
CCCCHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
LLAAFQQQRREKFSPALRWLDLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRFDLIDLDCFGCPNAFLQPVL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
QVLASEGFLLLSSSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHH
GRGLEPLVSFSEGRTFRVAVRIKRRISACEESHLGLLARCEACGAQLAQPLLSLQGWSGC
HCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
MCSQGGGRWAVSGPLWLGPLQSPVVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVY
EECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
CWSTAELAKRLVLSGPPSVLALVRALRFAGHEACGSAVMAGQLRTDATVTELLRICHEMC
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCHHHHHCCCCCCHHHHHHHHHHHHHH
QEGR
HCCC
>Mature Secondary Structure 
GFAPVSTCLLNGPLGSLEVSCKQSGLNQPTTHYREGSVVLEPGGGFFRPESRPSRDLSV
CCCHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
LLAAFQQQRREKFSPALRWLDLMAGCGIRALRWGLEAVKPSLLASGVEQLPELWVNDADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ERASLLERNLAPLRQEQISLKLFHQPAEVLLSRAYLDQCRFDLIDLDCFGCPNAFLQPVL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
QVLASEGFLLLSSSDGRSPTGHDRPGAIRSLAAAARVHPASWEIALRLQLGVLARHAWLL
HHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHH
GRGLEPLVSFSEGRTFRVAVRIKRRISACEESHLGLLARCEACGAQLAQPLLSLQGWSGC
HCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
MCSQGGGRWAVSGPLWLGPLQSPVVLADLLVLAEQSIVPIASATLRLLHRLQSDIGLPVY
EECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
CWSTAELAKRLVLSGPPSVLALVRALRFAGHEACGSAVMAGQLRTDATVTELLRICHEMC
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCHHHHHCCCCCCHHHHHHHHHHHHHH
QEGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]