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The map label for this gene is folD [H]

Identifier: 33863381

GI number: 33863381

Start: 1202106

End: 1203014

Strand: Reverse

Name: folD [H]

Synonym: PMT1110

Alternate gene names: 33863381

Gene position: 1203014-1202106 (Counterclockwise)

Preceding gene: 33863383

Following gene: 33863380

Centisome position: 49.9

GC content: 56.0

Gene sequence:

>909_bases
ATGGCCCTTAGGCTTGATGGCAAACAACTGGCCGCAGAATTGGAGCAGCGTCTCCAAGCTGAAATTGCGGCTGGCTTGGT
GCAAGCCGGAAGGCCGCCAGGGTTAGCTGTGTTGCGTATTGGGGACGACCCCGCCAGTGGGGTGTATGTGGCCAACAAGC
AGAAAGCTTGTGGGCGTATTGGCGTGGCCAGCCATCTAACCCATTTGCCGGAAACGGTTTCAGTTTCAAAGGTTTTGGCC
ACGATCCAGGCCTTAAACATCGATGAGCGAGTGGACGGCATTCTTTTGCAATTGCCTCTACCCAAGGGCCTTGATGAAGG
GCCTTTGCTGGCTGCCATTGATCCCGAGAAGGATGCCGATGGTCTGCATACCCTCAACCTTGGCCGATTGTTGAAAGGTG
AACCTGGGCCTCGTAGTTGTACCCCTGCCGGTGTGATGGCCTTGTTGGCACGCCATCAGATTTCTCTGGAGCGGAAAAGG
GCTGTTGTGATTGGGCGCAGCATCCTGGTAGGCCAACCAATGGCATTGATGCTTCAGGCGGCCAATGCCACTGTGAGCGT
GGCCCATTCTCATACCGGTGATCTGGCCTCGTTAACTCAGCAGGCCGATGTGTTGGTGGTGGCGGCAGGGCGAGCGCGGA
TGATTGGCGCAGAGCATGTCAAACCCGGAGCAGTGGTTGTCGATGTTGGCATTCATCGCCTTCCCCTAGATCCTGAACTA
GGGACGCAAGCCAAGGCCAGGTTGTGTGGAGATGTACGAACGCAAGAAGTTGAACCTTTAGCAAGTGCATTGACCCCTGT
CCCTGGTGGTGTAGGTCCGATGACCGTGACCATGCTCTTGGTGAATACCGTGGCTAGATGGCAGCAGCATTGCGGTTTGC
CCTTCGGCCTGAGAGATTTGCTGGTTTGA

Upstream 100 bases:

>100_bases
GAGCCTGTTTAAGGTTGGAAGACGGACCACAGAAGCTGGCACTTGTCCTAAACGCTAGCTCTCTTCTGCGTGCATGCTTG
GGGAAGCATCGGATCGGTGA

Downstream 100 bases:

>100_bases
GACTGGTTGGCCTGAGAGAATCCCATGAACGAGACAAGGTTCATGGCGGCGGCGGTCAGCACCTTCTTCGACTTCCCTGC
TTATTTGGCTGCGGCCAAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MALRLDGKQLAAELEQRLQAEIAAGLVQAGRPPGLAVLRIGDDPASGVYVANKQKACGRIGVASHLTHLPETVSVSKVLA
TIQALNIDERVDGILLQLPLPKGLDEGPLLAAIDPEKDADGLHTLNLGRLLKGEPGPRSCTPAGVMALLARHQISLERKR
AVVIGRSILVGQPMALMLQAANATVSVAHSHTGDLASLTQQADVLVVAAGRARMIGAEHVKPGAVVVDVGIHRLPLDPEL
GTQAKARLCGDVRTQEVEPLASALTPVPGGVGPMTVTMLLVNTVARWQQHCGLPFGLRDLLV

Sequences:

>Translated_302_residues
MALRLDGKQLAAELEQRLQAEIAAGLVQAGRPPGLAVLRIGDDPASGVYVANKQKACGRIGVASHLTHLPETVSVSKVLA
TIQALNIDERVDGILLQLPLPKGLDEGPLLAAIDPEKDADGLHTLNLGRLLKGEPGPRSCTPAGVMALLARHQISLERKR
AVVIGRSILVGQPMALMLQAANATVSVAHSHTGDLASLTQQADVLVVAAGRARMIGAEHVKPGAVVVDVGIHRLPLDPEL
GTQAKARLCGDVRTQEVEPLASALTPVPGGVGPMTVTMLLVNTVARWQQHCGLPFGLRDLLV
>Mature_301_residues
ALRLDGKQLAAELEQRLQAEIAAGLVQAGRPPGLAVLRIGDDPASGVYVANKQKACGRIGVASHLTHLPETVSVSKVLAT
IQALNIDERVDGILLQLPLPKGLDEGPLLAAIDPEKDADGLHTLNLGRLLKGEPGPRSCTPAGVMALLARHQISLERKRA
VVIGRSILVGQPMALMLQAANATVSVAHSHTGDLASLTQQADVLVVAAGRARMIGAEHVKPGAVVVDVGIHRLPLDPELG
TQAKARLCGDVRTQEVEPLASALTPVPGGVGPMTVTMLLVNTVARWQQHCGLPFGLRDLLV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=40.2730375426621, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI222136639, Length=285, Percent_Identity=39.6491228070175, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=38.9078498293515, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=27.1428571428571, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=45.4225352112676, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17568735, Length=261, Percent_Identity=39.463601532567, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319558, Length=283, Percent_Identity=40.6360424028269, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=38.9078498293515, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=42.6056338028169, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=42.6056338028169, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=42.6056338028169, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=42.6056338028169, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17136816, Length=301, Percent_Identity=42.1926910299003, Blast_Score=201, Evalue=3e-52,
Organism=Drosophila melanogaster, GI17136818, Length=301, Percent_Identity=42.1926910299003, Blast_Score=201, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31606; Mature: 31475

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRLDGKQLAAELEQRLQAEIAAGLVQAGRPPGLAVLRIGDDPASGVYVANKQKACGRI
CEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCC
GVASHLTHLPETVSVSKVLATIQALNIDERVDGILLQLPLPKGLDEGPLLAAIDPEKDAD
CHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCC
GLHTLNLGRLLKGEPGPRSCTPAGVMALLARHQISLERKRAVVIGRSILVGQPMALMLQA
CCEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECHHHHCCCHHHHEEC
ANATVSVAHSHTGDLASLTQQADVLVVAAGRARMIGAEHVKPGAVVVDVGIHRLPLDPEL
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCEEECCCCCCCCCEEEEECCCEECCCCCCC
GTQAKARLCGDVRTQEVEPLASALTPVPGGVGPMTVTMLLVNTVARWQQHCGLPFGLRDL
CCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LV
CC
>Mature Secondary Structure 
ALRLDGKQLAAELEQRLQAEIAAGLVQAGRPPGLAVLRIGDDPASGVYVANKQKACGRI
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCC
GVASHLTHLPETVSVSKVLATIQALNIDERVDGILLQLPLPKGLDEGPLLAAIDPEKDAD
CHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCC
GLHTLNLGRLLKGEPGPRSCTPAGVMALLARHQISLERKRAVVIGRSILVGQPMALMLQA
CCEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECHHHHCCCHHHHEEC
ANATVSVAHSHTGDLASLTQQADVLVVAAGRARMIGAEHVKPGAVVVDVGIHRLPLDPEL
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCEEECCCCCCCCCEEEEECCCEECCCCCCC
GTQAKARLCGDVRTQEVEPLASALTPVPGGVGPMTVTMLLVNTVARWQQHCGLPFGLRDL
CCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA