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The map label for this gene is umuD [H]

Identifier: 33862908

GI number: 33862908

Start: 688212

End: 688706

Strand: Direct

Name: umuD [H]

Synonym: PMT0635

Alternate gene names: 33862908

Gene position: 688212-688706 (Clockwise)

Preceding gene: 33862907

Following gene: 33862911

Centisome position: 28.55

GC content: 56.16

Gene sequence:

>495_bases
TTGACGTCCGATGTCGCGATGGAGTTCAGCGAGCCTCTTCTTCATTTGCGCTCCGAGCGGAAGCAGCAGATCCTGCCATT
AGCGAGTGAGCACGTGGCGGCAGGCTTCCCCTCCCCTGCTGAGGACTATATCGAGATCGGTATCGACCTCAACGAACAGT
TGATTCGTCATCCGGCTAGCACCTTCTTCCTGCGTGTTAGCGGCCACTCAATGACAGGTGTTGGCATCCACAATGGCGAC
CTCTTGGTTGTCGACCGCAGCCTAGATGCACGCCCCGGTCACATCGTGGTGGCGATTCTTGATGGTGCCTTCACACTCAA
ACAACTTGTCCGCCACCGTGGTGTACTGCGTCTAGAGGCCGCCCATTCCAATTACCCACCGCTGGATCTGGCCTGCTTCG
GTGACGTGCAAATCTGGGGTGTGGCAGTGCATTCCATTCATAGCCTCAACCCTGCGCAGCAAATATCCCACTCAACACAA
CGCCTACATGAATAG

Upstream 100 bases:

>100_bases
AGGCACGAGCGATCCTGTAAAGAGGTTGCTACCGCCTGGGGACACACCCCTATCAAGCCAAATCAGAAAATCACCCTGCT
GGAACGGGTGTACTATCGGG

Downstream 100 bases:

>100_bases
GCTCAAAATAGGCAAGACGATGAATAAGGCTGCCCGGCATAGCAATAGTGTTGTTGCTCACCTTTAAAGATATTGCCTTT
AAATCGTATATAGAGATAGG

Product: SOS mutagenesis protein UmuD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTSDVAMEFSEPLLHLRSERKQQILPLASEHVAAGFPSPAEDYIEIGIDLNEQLIRHPASTFFLRVSGHSMTGVGIHNGD
LLVVDRSLDARPGHIVVAILDGAFTLKQLVRHRGVLRLEAAHSNYPPLDLACFGDVQIWGVAVHSIHSLNPAQQISHSTQ
RLHE

Sequences:

>Translated_164_residues
MTSDVAMEFSEPLLHLRSERKQQILPLASEHVAAGFPSPAEDYIEIGIDLNEQLIRHPASTFFLRVSGHSMTGVGIHNGD
LLVVDRSLDARPGHIVVAILDGAFTLKQLVRHRGVLRLEAAHSNYPPLDLACFGDVQIWGVAVHSIHSLNPAQQISHSTQ
RLHE
>Mature_163_residues
TSDVAMEFSEPLLHLRSERKQQILPLASEHVAAGFPSPAEDYIEIGIDLNEQLIRHPASTFFLRVSGHSMTGVGIHNGDL
LVVDRSLDARPGHIVVAILDGAFTLKQLVRHRGVLRLEAAHSNYPPLDLACFGDVQIWGVAVHSIHSLNPAQQISHSTQR
LHE

Specific function: Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template [H]

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1787431, Length=130, Percent_Identity=42.3076923076923, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790476, Length=110, Percent_Identity=37.2727272727273, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 18072; Mature: 17941

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDVAMEFSEPLLHLRSERKQQILPLASEHVAAGFPSPAEDYIEIGIDLNEQLIRHPAS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHEEECCCCCHHHHHCCCC
TFFLRVSGHSMTGVGIHNGDLLVVDRSLDARPGHIVVAILDGAFTLKQLVRHRGVLRLEA
EEEEEECCCCEEEEEEECCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHCCEEEEEE
AHSNYPPLDLACFGDVQIWGVAVHSIHSLNPAQQISHSTQRLHE
CCCCCCCEEEEEECCEEEEHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSDVAMEFSEPLLHLRSERKQQILPLASEHVAAGFPSPAEDYIEIGIDLNEQLIRHPAS
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHEEECCCCCHHHHHCCCC
TFFLRVSGHSMTGVGIHNGDLLVVDRSLDARPGHIVVAILDGAFTLKQLVRHRGVLRLEA
EEEEEECCCCEEEEEEECCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHCCEEEEEE
AHSNYPPLDLACFGDVQIWGVAVHSIHSLNPAQQISHSTQRLHE
CCCCCCCEEEEEECCEEEEHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]