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The map label for this gene is glnA [H]

Identifier: 33862874

GI number: 33862874

Start: 657545

End: 658966

Strand: Direct

Name: glnA [H]

Synonym: PMT0601

Alternate gene names: 33862874

Gene position: 657545-658966 (Clockwise)

Preceding gene: 33862872

Following gene: 33862879

Centisome position: 27.27

GC content: 51.83

Gene sequence:

>1422_bases
ATGGGCAAATCACCCCAAGACGTCCTCCGCCAGATCAAGGACGAGGGCATTGAACTGATTGACCTCAAGTTCACTGATCT
GCACGGCAAGTGGCAGCATCTCACCGTTTGTTCGGATTTGATTGATGAGGAAGCCTTTGCGAATGGCTTGGCTTTCGATG
GATCTTCCATCCGTGGATGGAAGGCCATTAACGAATCAGATATGGATATGGTGCCCGACGCCAGTACGGCCTGGATCGAC
CCCTTTTATCGCCACAAGACTCTTAGCTTGATCTGTTCCATTCGGGAACCTCGTAGTGGTGAGCCCTATGCCAGATGCCC
CAGGGCGCTTGCCCAAAAAGCCCTCGGTTACTTAGCCGGCACCGGCCTGGCCGATACTGCTTTTTTCGGCCCTGAGCCAG
AGTTTTTCATCTTTGACGACGTCCGCTACAACTCTGGCGAAGGTGGTTGCTTCTACAGCGTTGACACGATTGAGGCCCCT
TGGAATAGCGGTCGCATTGAAGAAGGCGGCAACCTTGCCTACAAGATCCAACTGAAAGAGGGCTACTTTCCGGTTCCACC
CAACGACACCGCCCAGGACATCCGTTCCGAAATGCTGCTGCTGATGGGGCAATTAGGAATCCCGATGGAGAAGCACCACC
ATGAAGTTGCAGGTGCTGGCCAACATGAATTGGGGATGAAGTTCGCTGAGCTGATTGAAGCTGCGGACAACGTGATGATT
TACAAGTACATCGTCAGGAATGTTGCACGTAAATACGGCAAAACGGCAACATTTATGCCCAAGCCGGTGTTCAACGACAA
CGGCACCGGGATGCATGTTCACCAGAGCCTCTTTAAAGGAGGCCAACCCCTGTTCTTTGGAGAAGGCACCTACGCCAACT
TGTCACAGACGGCTCGCTGGTATATCGGCGGCATCCTTAAGCATGCTCCCTCCTTCCTGGCTTTCACAAACCCCACAACC
AATAGCTACAAGCGTTTGGTGCCAGGTTTTGAAGCTCCTGTAAACCTTGTTTATTCTCAGGGCAATAGGTCTGCAGCTGT
TCGCATTCCGCTCACTGGCCCAAACCCGAAAGCTAAGCGTCTTGAATTCCGCCCAGGCGATGCCTTGGCCAATCCTTATC
TAGCTTTCAGCGCAATGATGATGGCGGGCGTTGACGGCATTAAAAACCAAATTGACCCTGGCGATGGTTTCGATGAGGAT
CTATTTGAACTACCAGAAGAACGCCTTGCCAGCATCCCCACAGTGCCAGCTTCACTTAATGGTGCACTCGAAGCGTTAAA
TGCAGACAAGAACTACCTCATGGAAGGGGGAGTCTTCACTGAAGACTTCATTGACAACTGGATTGACATCAAATACGAAG
AGGTGCAGCAATTGCGTCAACGGCCTCATCCCCATGAGTTCACCATGTATTACGACGCATGA

Upstream 100 bases:

>100_bases
GGAAACAAGGCTTCAGTAGAAGGTACCTGTTGCTACAAAAAGGGGGCCATGCGGCTGATTACGGTCGCTCTCTTATCGCC
GGCTCGGCCTCATCTTCGCC

Downstream 100 bases:

>100_bases
GGTTGGGTGAAAAGATCACCATCTGTTTGAAAAAATAGGGAAAGTTATTCATTTAAACTAATTTTCAAGTCCCCGCACCT
AAGCGGGGACTTTTTAATGA

Product: glutamine synthetase, glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MGKSPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGWKAINESDMDMVPDASTAWID
PFYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAGTGLADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAP
WNSGRIEEGGNLAYKIQLKEGYFPVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMI
YKYIVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKRLEFRPGDALANPYLAFSAMMMAGVDGIKNQIDPGDGFDED
LFELPEERLASIPTVPASLNGALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA

Sequences:

>Translated_473_residues
MGKSPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGWKAINESDMDMVPDASTAWID
PFYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAGTGLADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAP
WNSGRIEEGGNLAYKIQLKEGYFPVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMI
YKYIVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKRLEFRPGDALANPYLAFSAMMMAGVDGIKNQIDPGDGFDED
LFELPEERLASIPTVPASLNGALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA
>Mature_472_residues
GKSPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGWKAINESDMDMVPDASTAWIDP
FYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAGTGLADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAPW
NSGRIEEGGNLAYKIQLKEGYFPVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMIY
KYIVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARWYIGGILKHAPSFLAFTNPTTN
SYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKRLEFRPGDALANPYLAFSAMMMAGVDGIKNQIDPGDGFDEDL
FELPEERLASIPTVPASLNGALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=476, Percent_Identity=23.9495798319328, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=53.4188034188034, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI87081870, Length=178, Percent_Identity=33.1460674157303, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52689; Mature: 52558

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGW
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCC
KAINESDMDMVPDASTAWIDPFYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAG
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGLADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAPWNSGRIEEGGNLAYKIQLKE
CCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCEECCCCCCCCCCCCCEEEEEEECC
GYFPVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMI
CEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEH
YKYIVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARW
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKR
HHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCE
LEFRPGDALANPYLAFSAMMMAGVDGIKNQIDPGDGFDEDLFELPEERLASIPTVPASLN
EECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCCCCCCCHHH
GALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA
HHHHHHCCCCHHEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
GKSPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGW
CCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCC
KAINESDMDMVPDASTAWIDPFYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAG
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGLADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAPWNSGRIEEGGNLAYKIQLKE
CCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCEECCCCCCCCCCCCCEEEEEEECC
GYFPVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMI
CEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEH
YKYIVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARW
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKR
HHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCE
LEFRPGDALANPYLAFSAMMMAGVDGIKNQIDPGDGFDEDLFELPEERLASIPTVPASLN
EECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHCCCCCCCHHH
GALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA
HHHHHHCCCCHHEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678591 [H]