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The map label for this gene is sir [H]

Identifier: 33862852

GI number: 33862852

Start: 634738

End: 636534

Strand: Direct

Name: sir [H]

Synonym: PMT0579

Alternate gene names: 33862852

Gene position: 634738-636534 (Clockwise)

Preceding gene: 33862850

Following gene: 33862864

Centisome position: 26.33

GC content: 52.92

Gene sequence:

>1797_bases
GTGACCGAAGGGCAGACAACATCTGGCCAAGCGGCAGATCTCAAGTTCGCAGCTTGTCTCGCCAGTGGAGCAGAGCAGAG
CAAATTTGAACAATTCAAGGCTGACAGCTGCTACTTACTAGACCCCCTGGCTGCTGAACTTGAGAACAGCAGTGATCACT
TCAGCAACGACGCCGTACAGCTGCTCAAATTCCACGGAAGCTATCAACAGGACAACCGAGATAACCGCCAGAAAGGTGCG
GGTAAGGACTGGCAAATGATGCTGCGTCTGCGCAGCCCTGGCGGACGTATTCCACCAGCATTGTTTTTAGCCTTAGATGA
TCTGTCCAATCGCCTTGGCAATGGGAGCCTGCGGGCCACTACCCGCCAGGCGTTCCAGATTCATGGGGTCCGCAAGGAGG
ACCTGCGGGAAGTTATCAGCACAATCATCCGATCAATGGGATCAACCCTGGCGGCTTGTGGTGATATCACTCGCAACGTT
ATGGCACCAGCAGCTCCCTATGAGAAAGGTGGTTATCCAGCTGCCCGACAACTTGCCAATGAAATCGCCGATGTTCTCAG
CCCAACGGCAGCGGAGGGGTCCTATCTGGACCTATGGGTGAATGGAAATGCGACCTACCGCATCCGCCCCTCCAGGCGAG
TGAAAAAAGTTCGTACCCGCCAGCATCAGGGCAACGTCTTCAGTGGAGATGCCAAGGAACCTCTCTATGGCACGACCTAC
TTGCCGCGCAAATTTAAGTGCGCCGTCACCGTGCCAGGCGATAACTCAGTTGATTTACTTACCAATGACATCGGTCTAGT
TGTCTTTACTGCTGCAAACGGTGAAATCAAGGGTTGCAATGTCTATGTGGGTGGCGGCATGGGTCGAACCCACAACAATG
AACAGACATTCGCCCGCACAGCTGATCCTCTTGGCTATGTGGCTGCAGAGCATGTATTGGATTTGGTGCAGTCGATTTTG
GCCTTGCAACGCGACTACGGAGACCGAAAGACAAGGCGTCATGCCCGCATGAAATATCTTCTCAATGATCATGGGATCAG
CTGGTTTAAACAGGAACTCAAGAGCAAATACTTCCTTCATCCAATCAAAGCGTTAAGGCCTGAACCAAAGAGCAAACTTG
AGGACTATCTGGGCTGGCATCAGCAAAGCGTTGGCCAATGGTTTGTTGGTTTGCCACTGCTCTCTGGTCGCTTGGAAGGA
GATCTAAAGAAAGGACTCCGCCAACTGGTAGAGACCTTTCAACTTGAAATTCGGCTAACACCGAATCAGGATCTATTGCT
CTGCAATATCGGGACGTCACAACGATCTAGTGTGCGCAAAGGTCTAGCCGCTCTAGGCATCCGCGCCCCAGAGGCTCCTG
CGATGCTGGCACGGCATGCAATGGCTTGCCCTGCGTTGCCCTTATGTGGTCTGGCGGTAACCGAAGCAGAGAGAACGCTG
CCTGCCTTGCTTGAGCGAATTGATGCTCAGCTGCGACGTCTTGAAATTACTAAATCAATCCTGATCCGCATGACAGGTTG
TCCTAATGGATGCGCTCGGCCCTATATGGCTGAGCTTGCTCTAGTGGGAAGTGGTGTCGATCAATATCAACTCTGGCTGG
GAGGCACCCCAAATCTGCAACGTCTTGCCAAACCCTATTTACAGCGGATGCCCCTCGATCAACTCGAGAGCACGCTTGAG
CCCCTATTGCTGAGCTGGAAGCAAGCTGGTGGTCGACGCAGCCTCGGAGATCATGTCAACAAACTTGGCGATCAAGCCGT
CCTTGGTTTGCTGTCTGGTAATGGACAGGCTCCATAG

Upstream 100 bases:

>100_bases
ACACGGAGGCGAGCAATCAAGACCGCCCAACTGTATTGAAGGGAGCAGCTCAACAGGTCGTGTGCATTTCGCTACGGTGT
ATTAGGAAAATTTTGACGCC

Downstream 100 bases:

>100_bases
ATTGCTTCTGCGACACCCGTTCTCCACGCCCAGAGTTGATCTCCATAGCTGAAATGCCACCATTCATTCGGGTGCTGAGA
AAACCCAGCTTTACCCATGG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTEGQTTSGQAADLKFAACLASGAEQSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGA
GKDWQMMLRLRSPGGRIPPALFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNV
MAPAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGNATYRIRPSRRVKKVRTRQHQGNVFSGDAKEPLYGTTY
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSIL
ALQRDYGDRKTRRHARMKYLLNDHGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEG
DLKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAMACPALPLCGLAVTEAERTL
PALLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLE
PLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGNGQAP

Sequences:

>Translated_598_residues
MTEGQTTSGQAADLKFAACLASGAEQSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGA
GKDWQMMLRLRSPGGRIPPALFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNV
MAPAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGNATYRIRPSRRVKKVRTRQHQGNVFSGDAKEPLYGTTY
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSIL
ALQRDYGDRKTRRHARMKYLLNDHGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEG
DLKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAMACPALPLCGLAVTEAERTL
PALLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLE
PLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGNGQAP
>Mature_597_residues
TEGQTTSGQAADLKFAACLASGAEQSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQLLKFHGSYQQDNRDNRQKGAG
KDWQMMLRLRSPGGRIPPALFLALDDLSNRLGNGSLRATTRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVM
APAAPYEKGGYPAARQLANEIADVLSPTAAEGSYLDLWVNGNATYRIRPSRRVKKVRTRQHQGNVFSGDAKEPLYGTTYL
PRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFARTADPLGYVAAEHVLDLVQSILA
LQRDYGDRKTRRHARMKYLLNDHGISWFKQELKSKYFLHPIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGD
LKKGLRQLVETFQLEIRLTPNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAMACPALPLCGLAVTEAERTLP
ALLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQRLAKPYLQRMPLDQLESTLEP
LLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGNGQAP

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=567, Percent_Identity=32.4514991181658, Blast_Score=298, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6322597, Length=574, Percent_Identity=37.1080139372822, Blast_Score=333, Evalue=4e-92,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 65793; Mature: 65662

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGQTTSGQAADLKFAACLASGAEQSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQ
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCHHHHH
LLKFHGSYQQDNRDNRQKGAGKDWQMMLRLRSPGGRIPPALFLALDDLSNRLGNGSLRAT
HHHHCCCCCCCCCCHHHCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVMAPAAPYEKGGYPAARQLAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EIADVLSPTAAEGSYLDLWVNGNATYRIRPSRRVKKVRTRQHQGNVFSGDAKEPLYGTTY
HHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCCCC
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFART
CCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHH
ADPLGYVAAEHVLDLVQSILALQRDYGDRKTRRHARMKYLLNDHGISWFKQELKSKYFLH
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGDLKKGLRQLVETFQLEIRLT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEC
PNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAMACPALPLCGLAVTEAERTL
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
PALLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHH
RLAKPYLQRMPLDQLESTLEPLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGNGQAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHEECCCCCCC
>Mature Secondary Structure 
TEGQTTSGQAADLKFAACLASGAEQSKFEQFKADSCYLLDPLAAELENSSDHFSNDAVQ
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCHHHHH
LLKFHGSYQQDNRDNRQKGAGKDWQMMLRLRSPGGRIPPALFLALDDLSNRLGNGSLRAT
HHHHCCCCCCCCCCHHHCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRQAFQIHGVRKEDLREVISTIIRSMGSTLAACGDITRNVMAPAAPYEKGGYPAARQLAN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EIADVLSPTAAEGSYLDLWVNGNATYRIRPSRRVKKVRTRQHQGNVFSGDAKEPLYGTTY
HHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCCCC
LPRKFKCAVTVPGDNSVDLLTNDIGLVVFTAANGEIKGCNVYVGGGMGRTHNNEQTFART
CCCEEEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHH
ADPLGYVAAEHVLDLVQSILALQRDYGDRKTRRHARMKYLLNDHGISWFKQELKSKYFLH
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PIKALRPEPKSKLEDYLGWHQQSVGQWFVGLPLLSGRLEGDLKKGLRQLVETFQLEIRLT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEC
PNQDLLLCNIGTSQRSSVRKGLAALGIRAPEAPAMLARHAMACPALPLCGLAVTEAERTL
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
PALLERIDAQLRRLEITKSILIRMTGCPNGCARPYMAELALVGSGVDQYQLWLGGTPNLQ
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHH
RLAKPYLQRMPLDQLESTLEPLLLSWKQAGGRRSLGDHVNKLGDQAVLGLLSGNGQAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]