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The map label for this gene is proB

Identifier: 33862802

GI number: 33862802

Start: 576861

End: 577946

Strand: Direct

Name: proB

Synonym: PMT0529

Alternate gene names: 33862802

Gene position: 576861-577946 (Clockwise)

Preceding gene: 33862801

Following gene: 33862803

Centisome position: 23.93

GC content: 57.27

Gene sequence:

>1086_bases
ATGAGTCTGTGGGTGGTGAAGGTGGGAACCAGCCTGCTGAGAGGCAATGAGAAGCAATCCACCGCCGAAGTGATTGAGAG
CTACAGCGCCTGCCTTTCAGCAAGCCTCAAGCGGGGGGATCATGTTGTCCTCGTAACAAGTGGCGCCGTAGGTCTTGGTT
GTAATCGTCTAGGGCTAAGCCAACGGCCGGTCGATGTGGTGGCTCTCCAAGGGACGGCGGCGATCGGTCAAGGACAACTC
ATGGCTCTTTATGAGGCTGCCATGAGCCGAAGGGGTCACACCGTGGCTCAGGTATTGCTTACCCGCTCAGATCTTGGCTC
CAGGCAGCGATACCGCAATGCTTCTAGCACCTTGAAGCAATTGCTCGACTGGGGCGTTCTGCCGGTGGTGAATGAGAACG
ACGCCCTCTCTCCGGAAGAATTGCGATACGGCGACAACGACACCCTTTCAGCTCTCGTTGCAACCGCAGTGGAGGCTGAT
CAGCTCATCATGCTCACCGACGTGGATCGCCTTTATTCCTCAGACCCCCGCATTAATGCCTCTGCCGAACCGATCTCAGA
TGTTTACCATCCCCATGAACTGACTGCCCTGGAAGTCGCGGCAGGGGAGGGAGGTGCCTGGGGCACTGGGGGCATGACTA
CAAAACTTGCGGCAGCTCGCATCGCTACCGCAAGTGGCATCACCGTTCTTCTTGCTGATGGCCGCGATCCTCAAGTGCTT
GATGGGTTGCTGCAAGGAAGACGGAGCGGCACGGTGTTTCATCCTCACCCTCAACCTCTCGGCAATCGCAAGAGCTGGCT
GGCCCATGCTCTGAAGCCCTTAGGGACCCTTCAGCTGGATGAAGGCGCCTGCGATGCCCTTCAACATCGTGGCGCCTCCC
TGCTCTTGGTGGGGGTTAAAGAGGTGGAAGGAAACTTTGAAGCCAACCAGCCGGTAAGACTAATTAATCCCGAAGGTAAC
GAGCTCGCTCGAGGCCTGTGTTCCTTGAGTAGCAAAGAACTGCGCGAGGCCATTGAGACCCAAATGAGCACGAATCGTTC
ACCGGTCGTGGTGCATAGGGACGTGTTGGTTCTCCGCAACGCGTGA

Upstream 100 bases:

>100_bases
TTCTAGTTCGACCACTACGCGCCGATGGCTACCCCTGCAAAAACAACAGAGTTCAGCGTCTTGAACAAAAACTTGCCGCA
TGGCTAGGGGCCCCACAGAC

Downstream 100 bases:

>100_bases
CTTGATTGAACATCACCAACACATCACGCCTACGATCCGCCGAAAAGTGATTTGACATGCGCTTCAGCCAGTTGATCGCC
TCCCTCCAACAAGGAAGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQGTAAIGQGQL
MALYEAAMSRRGHTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEAD
QLIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGAWGTGGMTTKLAAARIATASGITVLLADGRDPQVL
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGN
ELARGLCSLSSKELREAIETQMSTNRSPVVVHRDVLVLRNA

Sequences:

>Translated_361_residues
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQGTAAIGQGQL
MALYEAAMSRRGHTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEAD
QLIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGAWGTGGMTTKLAAARIATASGITVLLADGRDPQVL
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGN
ELARGLCSLSSKELREAIETQMSTNRSPVVVHRDVLVLRNA
>Mature_360_residues
SLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQGTAAIGQGQLM
ALYEAAMSRRGHTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQ
LIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGAWGTGGMTTKLAAARIATASGITVLLADGRDPQVLD
GLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGNE
LARGLCSLSSKELREAIETQMSTNRSPVVVHRDVLVLRNA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=282, Percent_Identity=31.5602836879433, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI21361368, Length=284, Percent_Identity=31.3380281690141, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=41.9178082191781, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17569969, Length=265, Percent_Identity=32.0754716981132, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17569967, Length=265, Percent_Identity=32.0754716981132, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=32.2033898305085, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=349, Percent_Identity=35.8166189111748, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21357643, Length=285, Percent_Identity=31.2280701754386, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_PROMM (Q7V844)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894362.1
- ProteinModelPortal:   Q7V844
- STRING:   Q7V844
- GeneID:   1729544
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0529
- NMPDR:   fig|74547.1.peg.529
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   PMAR74547:PMT0529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38511; Mature: 38380

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLS
CEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
QRPVDVVALQGTAAIGQGQLMALYEAAMSRRGHTVAQVLLTRSDLGSRQRYRNASSTLKQ
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHH
LLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQLIMLTDVDRLYSSDPRINA
HHHCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
SAEPISDVYHPHELTALEVAAGEGGAWGTGGMTTKLAAARIATASGITVLLADGRDPQVL
CCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVK
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEE
EVEGNFEANQPVRLINPEGNELARGLCSLSSKELREAIETQMSTNRSPVVVHRDVLVLRN
ECCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
A
C
>Mature Secondary Structure 
SLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLS
EEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
QRPVDVVALQGTAAIGQGQLMALYEAAMSRRGHTVAQVLLTRSDLGSRQRYRNASSTLKQ
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHH
LLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQLIMLTDVDRLYSSDPRINA
HHHCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
SAEPISDVYHPHELTALEVAAGEGGAWGTGGMTTKLAAARIATASGITVLLADGRDPQVL
CCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVK
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEE
EVEGNFEANQPVRLINPEGNELARGLCSLSSKELREAIETQMSTNRSPVVVHRDVLVLRN
ECCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642