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The map label for this gene is thrS [H]

Identifier: 33862697

GI number: 33862697

Start: 474279

End: 476201

Strand: Direct

Name: thrS [H]

Synonym: PMT0424

Alternate gene names: 33862697

Gene position: 474279-476201 (Clockwise)

Preceding gene: 33862696

Following gene: 33862698

Centisome position: 19.67

GC content: 43.37

Gene sequence:

>1923_bases
ATGCCTATTATTGCACTTCCAGACGGAAATAAAAAGAAGTTTGATCAACCCGTAACCATTATGGAGGTGGCCGAAAGCCT
TGGGCCTGGATTAGCAAAGGCGGCTATCGCAGGCCGAGTCAATGGCGTATTGCTTGATACCTGTATCCCTATCGAGAAAG
ACTCTGAAGTCAATATCATCACGGCGAAAGACCAAGATGGAATTGAGACTATTCGCCACTCATTCGCTCACTTGATTGGT
CATGCCGTAAAGCAATTATATCCCGAAGCAAAAATGGCCATTGGTCCAGTTATTGAAGATGGATTTTATTATGATATTGC
CTATGATCATCCTTTTACTCCTAAAGACTTGGAAGCGATTGAGGCTCGTATGAAAGAGCTGGTTAAGCTTGACTACGACG
TTAATGTTGAAATAGTTTCAAAGGAAGAGGCTCGTAAGGAATTCGAAAAGCGATGCGAGCCCTACAAGATCGAAATCGTA
GATGAAATTCCTGAGAATGAAATTATTAAGCTATATCGACATCAAGAATATACTGATATGTGTAGAGGTCCACATGTGCC
TAACACAAGGCATTTGCGCACTTTCAAATTAATGAAAGTATCAGGTGCTTATTGGCGAGGTGATTCAAATAAAACAATGT
TGCAGCGCATCTATGGCACGGCCTGGGGAAGTTCCAAAGAGCTAAAAGCTTATCTCAAGCGCCTTGAAGAAGCTGAAAAG
CGCGATCATCGCAGGATCGCCAAGCAAATGTCTTTATTTCATACTCAAGAAGAAGCTCCTGGGATGATTTTCTGGCATGC
CAAGGGTTGGGCTATTTATCAGGTTTTAGAGCAATATATTCGCGAGACCCTGAGCCTGCATGACTACCAAGAAATCCGAA
CACCTCAGGTTGTAGACCGCTCCTTATGGGAGAAATCAGGCCATTGGGAGAAGTTCAAAGATGACATGTTTACAACGACA
TCTGAAAATCGTGAATATGCCATAAAGCCTATGAATTGTCCCTGCCATGTACAGATCTTCAATCAAGGCCTAAAAAGTTA
CCGTGACCTGCCAATTAGATTGGCAGAGTTTGGTTCATGTTTAAGGAATGAACCATCGGGTTCACTTCATGGTCTCATGC
GCGTGCGCAATTTTGTTCAAGACGATGCTCACATCTTTTGTACTGAGCTTCAGGTTCAGGAAGAGGTTTCTAAGTTTATT
GATCTAGTCTTTGAGGTTTACAGATCATTTGGGTTTGACTCGGTGCTTATAAAGTTATCAACCAGGCCCGAAAAGCGTGT
TGGTAGTGATGAGATCTGGGACAAATCAGAGAAGGCCTTGTCCGATGCATTGGATGCTAAAGGTCTTGCATGGGACTTAT
TGCCAGGGGAAGGTGCATTCTACGGGCCTAAAATTGAGTTTTCTTTAAAAGACTGTCTTGGTAGAGTTTGGCAATGTGGA
ACGATCCAGGTTGACTTCTCGATGCCGGAACGTTTGGGAGCATCTTATGTAGCAGAAGACAGCCAGCGCAGAACACCAGT
AATGTTGCATCGAGCAATTCTGGGTTCTTTTGAACGTTTTATCGGAATTCTGATCGAGCACTATGCTGGACGACTGCCTA
TTTGGCTAGCACCTGTGCAGGTGGTAGTGATGGGGATTACAGACCGTAATGCTCAGGCTTGTCAGGATATTTGCAAGAAG
TTATCAGCTCTAGAATATCGAACTGAAGTTGACTTAAGAAATGAAAAAATTGGTTTTAAAGTTCGCGAGCATACTCTTCA
GCGTGTACCATTTTTAATCATTATTGGTGATAAAGAACAACAGAGTGGAGAGGTGGCTGTGCGCACTCGAGAGGGCAAGG
ACTTTGGCAGTATGCCTTTGAAAGGCTTCACATCACTTCTGGATGAAGCAATTGCTCTTAAAGGTAGATCAGGTGTCTCT
TGA

Upstream 100 bases:

>100_bases
GCAAAATATTTTAGTCCAGCATTCAGAGGTGCAAGGATTGCTAGTGATATAGCTGATAAAATAGAACACTATTTCAACTC
ATCAAAGAAAACTCCTTGCT

Downstream 100 bases:

>100_bases
CAAATGTTGTCAATAGTTAGGTGATAATAATAATTGAGGAATACCTCGCTTTGGAGTGAATCTCTCTTCCGTCTGGATGA
GAAGCTGAATAGCAAATTGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MPIIALPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIG
HAVKQLYPEAKMAIGPVIEDGFYYDIAYDHPFTPKDLEAIEARMKELVKLDYDVNVEIVSKEEARKEFEKRCEPYKIEIV
DEIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHDYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTT
SENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFI
DLVFEVYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRLPIWLAPVQVVVMGITDRNAQACQDICKK
LSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLKGFTSLLDEAIALKGRSGVS

Sequences:

>Translated_640_residues
MPIIALPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIG
HAVKQLYPEAKMAIGPVIEDGFYYDIAYDHPFTPKDLEAIEARMKELVKLDYDVNVEIVSKEEARKEFEKRCEPYKIEIV
DEIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHDYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTT
SENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFI
DLVFEVYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRLPIWLAPVQVVVMGITDRNAQACQDICKK
LSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLKGFTSLLDEAIALKGRSGVS
>Mature_639_residues
PIIALPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNIITAKDQDGIETIRHSFAHLIGH
AVKQLYPEAKMAIGPVIEDGFYYDIAYDHPFTPKDLEAIEARMKELVKLDYDVNVEIVSKEEARKEFEKRCEPYKIEIVD
EIPENEIIKLYRHQEYTDMCRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEKR
DHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHDYQEIRTPQVVDRSLWEKSGHWEKFKDDMFTTTS
ENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSCLRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFID
LVFEVYRSFGFDSVLIKLSTRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCGT
IQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRLPIWLAPVQVVVMGITDRNAQACQDICKKL
SALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQQSGEVAVRTREGKDFGSMPLKGFTSLLDEAIALKGRSGVS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=617, Percent_Identity=38.2495948136143, Blast_Score=451, Evalue=1e-126,
Organism=Homo sapiens, GI38570111, Length=620, Percent_Identity=38.3870967741935, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI20070344, Length=638, Percent_Identity=34.9529780564263, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI1788013, Length=632, Percent_Identity=62.0253164556962, Blast_Score=853, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=640, Percent_Identity=37.8125, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984192, Length=640, Percent_Identity=37.8125, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=628, Percent_Identity=36.9426751592357, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=37.9396984924623, Blast_Score=296, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24583839, Length=641, Percent_Identity=37.597503900156, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24583842, Length=641, Percent_Identity=37.597503900156, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI28574141, Length=641, Percent_Identity=37.597503900156, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73272; Mature: 73141

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIALPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNII
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
TAKDQDGIETIRHSFAHLIGHAVKQLYPEAKMAIGPVIEDGFYYDIAYDHPFTPKDLEAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCEEEEEEECCCCCCHHHHHH
EARMKELVKLDYDVNVEIVSKEEARKEFEKRCEPYKIEIVDEIPENEIIKLYRHQEYTDM
HHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHH
CRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
HCCCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHDYQEIRTPQVVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLWEKSGHWEKFKDDMFTTTSENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSC
HHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
LRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFIDLVFEVYRSFGFDSVLIKLS
HCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCCHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRLPIWLAPVQ
EEEEECCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEE
VVVMGITDRNAQACQDICKKLSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCC
QSGEVAVRTREGKDFGSMPLKGFTSLLDEAIALKGRSGVS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIIALPDGNKKKFDQPVTIMEVAESLGPGLAKAAIAGRVNGVLLDTCIPIEKDSEVNII
CEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
TAKDQDGIETIRHSFAHLIGHAVKQLYPEAKMAIGPVIEDGFYYDIAYDHPFTPKDLEAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEECCCEEEEEEECCCCCCHHHHHH
EARMKELVKLDYDVNVEIVSKEEARKEFEKRCEPYKIEIVDEIPENEIIKLYRHQEYTDM
HHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHH
CRGPHVPNTRHLRTFKLMKVSGAYWRGDSNKTMLQRIYGTAWGSSKELKAYLKRLEEAEK
HCCCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDHRRIAKQMSLFHTQEEAPGMIFWHAKGWAIYQVLEQYIRETLSLHDYQEIRTPQVVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLWEKSGHWEKFKDDMFTTTSENREYAIKPMNCPCHVQIFNQGLKSYRDLPIRLAEFGSC
HHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHHHHHHHHH
LRNEPSGSLHGLMRVRNFVQDDAHIFCTELQVQEEVSKFIDLVFEVYRSFGFDSVLIKLS
HCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPEKRVGSDEIWDKSEKALSDALDAKGLAWDLLPGEGAFYGPKIEFSLKDCLGRVWQCG
CCCCCCCCCCHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
TIQVDFSMPERLGASYVAEDSQRRTPVMLHRAILGSFERFIGILIEHYAGRLPIWLAPVQ
EEEEECCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEE
VVVMGITDRNAQACQDICKKLSALEYRTEVDLRNEKIGFKVREHTLQRVPFLIIIGDKEQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEECCCCC
QSGEVAVRTREGKDFGSMPLKGFTSLLDEAIALKGRSGVS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA