Click here to switch to the map view.

The map label for this gene is mak [H]

Identifier: 308535160

GI number: 308535160

Start: 448810

End: 449244

Strand: Direct

Name: mak [H]

Synonym: NA

Alternate gene names: 308535160

Gene position: 448810-449244 (Clockwise)

Preceding gene: 197116769

Following gene: 197116770

Centisome position: 9.72

GC content: 65.52

Gene sequence:

>435_bases
ATGCCGGCATCGGAACCGTACCGCATAGGAGTGGATCTGGGCGGGACCAAGACGGAGGTCGTGGTGCTCGATCCGCAGGA
CGGGGTTGTCTTCAGGGAAAGGAGGAAGACCCCGCTGGTTGAGGGATACCATGCCGTGCTGAACTGCGTGGCCGACCTGG
TGCGGGATGGGGCCTGCCGGGTGCCGGCGGCTAATAGCTGCACGGTCGGCATCGGCATCCCGGGCTCCGTCGATGCCGTC
AGCGGAATGGTGCGCAACGCCAACTCGGTCTGCCTTATAGGGCGCCCGTTGCAGGCCGATCTGGAGCGGCTTTTGGGTAG
GAGGATCGGGGTAAGAAACGACGCCGACTGCTTCGCCATGGCCGAATGCCGCAAGGGGGCGGGAGCGGGTTACGGCCTTG
TCTTCGGCGTCATCATGGGAACCGGTTGCGGCGGG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: D-fructose 6-kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:
NA

Sequences:

>Translated_145_residues
MPASEPYRIGVDLGGTKTEVVVLDPQDGVVFRERRKTPLVEGYHAVLNCVADLVRDGACRVPAANSCTVGIGIPGSVDAV
SGMVRNANSVCLIGRPLQADLERLLGRRIGVRNDADCFAMAECRKGAGAGYGLVFGVIMGTGCGG
>Mature_144_residues
PASEPYRIGVDLGGTKTEVVVLDPQDGVVFRERRKTPLVEGYHAVLNCVADLVRDGACRVPAANSCTVGIGIPGSVDAVS
GMVRNANSVCLIGRPLQADLERLLGRRIGVRNDADCFAMAECRKGAGAGYGLVFGVIMGTGCGG

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=137, Percent_Identity=44.5255474452555, Blast_Score=108, Evalue=1e-25,
Organism=Escherichia coli, GI1787363, Length=136, Percent_Identity=39.7058823529412, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 15064; Mature: 14932

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASEPYRIGVDLGGTKTEVVVLDPQDGVVFRERRKTPLVEGYHAVLNCVADLVRDGACR
CCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
VPAANSCTVGIGIPGSVDAVSGMVRNANSVCLIGRPLQADLERLLGRRIGVRNDADCFAM
CCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHH
AECRKGAGAGYGLVFGVIMGTGCGG
HHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PASEPYRIGVDLGGTKTEVVVLDPQDGVVFRERRKTPLVEGYHAVLNCVADLVRDGACR
CCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
VPAANSCTVGIGIPGSVDAVSGMVRNANSVCLIGRPLQADLERLLGRRIGVRNDADCFAM
CCCCCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHH
AECRKGAGAGYGLVFGVIMGTGCGG
HHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]