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The map label for this gene is dapF

Identifier: 30410829

GI number: 30410829

Start: 623300

End: 624109

Strand: Reverse

Name: dapF

Synonym: BUsg568

Alternate gene names: 30410829

Gene position: 624109-623300 (Counterclockwise)

Preceding gene: 21672828

Following gene: 21672825

Centisome position: 97.3

GC content: 25.43

Gene sequence:

>810_bases
ATGCATGGTTTAGGCAACGACTTTATGGTCATTGAATCGATTAAACAAGATTTTATTTTAACTAAATCAATTATTGAAAA
CTTATCTAATCGAAATACTGGAGTTGGTTTTGATCAATTATTATTAATTGAAAAATCTAATAACTTTAAATTTGATTTTC
ATTATCGTATTTTTAACGCCAATGGAAATGAAGTTGAACAATGTGGAAATGGTGCTCGATGTGTAGGTTTATTTCTTTTT
TTAAAAGGTTTAATCAACAAACGAAAAATTATGTTAAGTACTAATAAAAAAAATATATCTATTAAATTTATGTCTGATAA
CAATATACAAGTTAATATGAATGAACCTAGTTTTAAATTAGAAGAAATATCTATTTCAAAAAAAATTGTATTAAATAATT
TTTCTGTCAAACTTATAAATAAAAATTTAAAATGTAGTTTAGTATCTATTGGAAATCCTCATTGTGTTATTAAAGTAGAA
TCTATAAATAATGCACCTGTAAAAAAAATTGGTGAACTTTTAGAAAAAAATTCAATTTTTCCTCAAGGAATAAACGTAGG
TTTTATGAAAGTTATAAACAAAAATCACATTCAATTAAGAGTGTATGAACGTGATGTAGGTGAAACTCAATCTTGCGGTA
GTGGCGCGTGTGCAGCAGTTGCAGTTGGTATAGCCAAAAATCTTCTTTGTGATAATGTTAAGGTAGATTTATTAGGTGGT
AATTTAATAATTAAATGGAAAGGTTTTAATTATCCTCTTTATATGATAGGGACAGCTTGTCATATTTACGATGGATATAT
ATACATATAA

Upstream 100 bases:

>100_bases
TACATTGTTTTATTTTTAAGAATGTAAAAAGAATAAATAACTATCAGGAATAAAAATGTTTTCAAATTACAAAAAAAAGA
AAAAAATTTATTTTTCTAAG

Downstream 100 bases:

>100_bases
AAAACATATTTTATTTAAAAAATGTTTTTTATACTTTTGGAAAATAAAAAATGTTATTAAAAAAAAAAATACAAAAAAAT
TTAATCAAATTGTTTTATCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MHGLGNDFMVIESIKQDFILTKSIIENLSNRNTGVGFDQLLLIEKSNNFKFDFHYRIFNANGNEVEQCGNGARCVGLFLF
LKGLINKRKIMLSTNKKNISIKFMSDNNIQVNMNEPSFKLEEISISKKIVLNNFSVKLINKNLKCSLVSIGNPHCVIKVE
SINNAPVKKIGELLEKNSIFPQGINVGFMKVINKNHIQLRVYERDVGETQSCGSGACAAVAVGIAKNLLCDNVKVDLLGG
NLIIKWKGFNYPLYMIGTACHIYDGYIYI

Sequences:

>Translated_269_residues
MHGLGNDFMVIESIKQDFILTKSIIENLSNRNTGVGFDQLLLIEKSNNFKFDFHYRIFNANGNEVEQCGNGARCVGLFLF
LKGLINKRKIMLSTNKKNISIKFMSDNNIQVNMNEPSFKLEEISISKKIVLNNFSVKLINKNLKCSLVSIGNPHCVIKVE
SINNAPVKKIGELLEKNSIFPQGINVGFMKVINKNHIQLRVYERDVGETQSCGSGACAAVAVGIAKNLLCDNVKVDLLGG
NLIIKWKGFNYPLYMIGTACHIYDGYIYI
>Mature_269_residues
MHGLGNDFMVIESIKQDFILTKSIIENLSNRNTGVGFDQLLLIEKSNNFKFDFHYRIFNANGNEVEQCGNGARCVGLFLF
LKGLINKRKIMLSTNKKNISIKFMSDNNIQVNMNEPSFKLEEISISKKIVLNNFSVKLINKNLKCSLVSIGNPHCVIKVE
SINNAPVKKIGELLEKNSIFPQGINVGFMKVINKNHIQLRVYERDVGETQSCGSGACAAVAVGIAKNLLCDNVKVDLLGG
NLIIKWKGFNYPLYMIGTACHIYDGYIYI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=269, Percent_Identity=49.0706319702602, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BUCAP (Q8K901)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660893.2
- ProteinModelPortal:   Q8K901
- SMR:   Q8K901
- EnsemblBacteria:   EBBUCT00000000056
- GeneID:   1005624
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg568
- GeneTree:   EBGT00050000008129
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   BAPH198804:BUSG568-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30103; Mature: 30103

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 83-83 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGLGNDFMVIESIKQDFILTKSIIENLSNRNTGVGFDQLLLIEKSNNFKFDFHYRIFNA
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEEEECC
NGNEVEQCGNGARCVGLFLFLKGLINKRKIMLSTNKKNISIKFMSDNNIQVNMNEPSFKL
CCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCEEEEECCCCEEE
EEISISKKIVLNNFSVKLINKNLKCSLVSIGNPHCVIKVESINNAPVKKIGELLEKNSIF
EEEECEEEEEEECEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCC
PQGINVGFMKVINKNHIQLRVYERDVGETQSCGSGACAAVAVGIAKNLLCDNVKVDLLGG
CCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECC
NLIIKWKGFNYPLYMIGTACHIYDGYIYI
EEEEEEECCCCCEEEEEEEEEEECCEEEC
>Mature Secondary Structure
MHGLGNDFMVIESIKQDFILTKSIIENLSNRNTGVGFDQLLLIEKSNNFKFDFHYRIFNA
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEEEECC
NGNEVEQCGNGARCVGLFLFLKGLINKRKIMLSTNKKNISIKFMSDNNIQVNMNEPSFKL
CCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCEEEEECCCCEEE
EEISISKKIVLNNFSVKLINKNLKCSLVSIGNPHCVIKVESINNAPVKKIGELLEKNSIF
EEEECEEEEEEECEEEEEECCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCC
PQGINVGFMKVINKNHIQLRVYERDVGETQSCGSGACAAVAVGIAKNLLCDNVKVDLLGG
CCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECC
NLIIKWKGFNYPLYMIGTACHIYDGYIYI
EEEEEEECCCCCEEEEEEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438