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The map label for this gene is thiF [H]

Identifier: 30263515

GI number: 30263515

Start: 3328622

End: 3329638

Strand: Reverse

Name: thiF [H]

Synonym: BA_3624

Alternate gene names: 30263515

Gene position: 3329638-3328622 (Counterclockwise)

Preceding gene: 30263516

Following gene: 30263514

Centisome position: 63.7

GC content: 36.58

Gene sequence:

>1017_bases
ATGGCTGAGCGGTATTCACGACAACAGTTGTTCAAACCGATTGGGGATAGAGGACAAGAAAAGATTCGAAATAAACATGT
GTTAATTGTAGGGGCAGGCGCATTAGGAAGTGCAAGTGCTGAAAGTTTCGTACGTGCAGGCATTGGGAAGTTGACGATTA
TTGATCGTGATTATGTTGAATGGAGTAATTTACAAAGACAACAACTGTACTCTGAAGAAGATGCGAGAGAGAAATTGCCA
AAAGCAATCGCTGCTAAAAATCGGCTAGAAAAACTTAATTCGGAAGTACAAATAGATGCTTTCGTAATGGATGCATGTGC
AGAAAACTTGGAAGGACTATTAGAAAATGTTGATGTAATAATTGATGCAACAGATAATTTCGATATCCGATTTATAATAA
ATGATTTATCACAAAAATATAATATCCCGTGGGTATATGGTTCTTGCGTTGGCTCGTACGGTATGAGTTATACAATTATT
CCGCAAGAGACACCGTGTTTACATTGTGTGCTGAAGAACGTTCCAGTTACAGGTGTGACGTGTGATACAGCTGGAATTAT
TAGTCCGACTGTTCAAATCGTTGCAGCATATCAAGTGGCGGAAGCACTAAAAATTTTAGTAGAAGATTTTGCAGCAATTA
GAAAAACATTTTTTATGTTTGATATATGGAGTAATCAAAACCATTTTATAAAACTAGGAAAAATCAAGACAGACGATTGC
CCTTCGTGCGGTTTGAATCGAACTTATCCTTATTTATCATACGAAAATCAAACGAAGGTAGCCGTTTTGTGCGGAAGAAA
TACAGTTCAAATTAGAACGGTAGAAAGTAGACAGTACAATTTTGATGATATAGAAAAAGTATTAAAAAAACTGGGGGAAG
TAGATCGGAATCCGTATTTACTATCTTGCCAACTAGATGAGTACCGCGTCGTTATTTTTCGAGATGGTCGTGTTTTCATT
CATGGTACAAATGATATTTCAAAAGCGAAACAGTTATATTATCGCGTATTCGGTTAA

Upstream 100 bases:

>100_bases
TGTAATTGGAGGAGCTGGATTTGTTGGATTTGCTTACTATGCTTGTTATCAAAAACAGCATTCTAATATGAAATAGAGTT
CGTGAGAGGAAGGGTATGTA

Downstream 100 bases:

>100_bases
TAGAAAGGGTTGTCAATAATGGAAAGAAGAGTGCCAATTACGGTTGAAGAAGCTGTTCGTAAAGTAATGGAATTTGCTAA
TGAGGGTTTAAAGGAGCTAC

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAERYSRQQLFKPIGDRGQEKIRNKHVLIVGAGALGSASAESFVRAGIGKLTIIDRDYVEWSNLQRQQLYSEEDAREKLP
KAIAAKNRLEKLNSEVQIDAFVMDACAENLEGLLENVDVIIDATDNFDIRFIINDLSQKYNIPWVYGSCVGSYGMSYTII
PQETPCLHCVLKNVPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFAAIRKTFFMFDIWSNQNHFIKLGKIKTDDC
PSCGLNRTYPYLSYENQTKVAVLCGRNTVQIRTVESRQYNFDDIEKVLKKLGEVDRNPYLLSCQLDEYRVVIFRDGRVFI
HGTNDISKAKQLYYRVFG

Sequences:

>Translated_338_residues
MAERYSRQQLFKPIGDRGQEKIRNKHVLIVGAGALGSASAESFVRAGIGKLTIIDRDYVEWSNLQRQQLYSEEDAREKLP
KAIAAKNRLEKLNSEVQIDAFVMDACAENLEGLLENVDVIIDATDNFDIRFIINDLSQKYNIPWVYGSCVGSYGMSYTII
PQETPCLHCVLKNVPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFAAIRKTFFMFDIWSNQNHFIKLGKIKTDDC
PSCGLNRTYPYLSYENQTKVAVLCGRNTVQIRTVESRQYNFDDIEKVLKKLGEVDRNPYLLSCQLDEYRVVIFRDGRVFI
HGTNDISKAKQLYYRVFG
>Mature_337_residues
AERYSRQQLFKPIGDRGQEKIRNKHVLIVGAGALGSASAESFVRAGIGKLTIIDRDYVEWSNLQRQQLYSEEDAREKLPK
AIAAKNRLEKLNSEVQIDAFVMDACAENLEGLLENVDVIIDATDNFDIRFIINDLSQKYNIPWVYGSCVGSYGMSYTIIP
QETPCLHCVLKNVPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFAAIRKTFFMFDIWSNQNHFIKLGKIKTDDCP
SCGLNRTYPYLSYENQTKVAVLCGRNTVQIRTVESRQYNFDDIEKVLKKLGEVDRNPYLLSCQLDEYRVVIFRDGRVFIH
GTNDISKAKQLYYRVFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=244, Percent_Identity=30.327868852459, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38045948, Length=155, Percent_Identity=31.6129032258064, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI150417996, Length=161, Percent_Identity=29.1925465838509, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=31.8367346938775, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082356, Length=250, Percent_Identity=34.4, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17540406, Length=250, Percent_Identity=32.4, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI193203301, Length=162, Percent_Identity=28.3950617283951, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=255, Percent_Identity=27.4509803921569, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=31.7647058823529, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582879, Length=242, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28573937, Length=182, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 38355; Mature: 38224

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERYSRQQLFKPIGDRGQEKIRNKHVLIVGAGALGSASAESFVRAGIGKLTIIDRDYVE
CCCHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCHHH
WSNLQRQQLYSEEDAREKLPKAIAAKNRLEKLNSEVQIDAFVMDACAENLEGLLENVDVI
HHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
IDATDNFDIRFIINDLSQKYNIPWVYGSCVGSYGMSYTIIPQETPCLHCVLKNVPVTGVT
EECCCCCEEEEEEEHHHHHCCCCEEEHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEE
CDTAGIISPTVQIVAAYQVAEALKILVEDFAAIRKTFFMFDIWSNQNHFIKLGKIKTDDC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCEECCCC
PSCGLNRTYPYLSYENQTKVAVLCGRNTVQIRTVESRQYNFDDIEKVLKKLGEVDRNPYL
CCCCCCCCCCEEEECCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
LSCQLDEYRVVIFRDGRVFIHGTNDISKAKQLYYRVFG
EEEEECCEEEEEEECCEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AERYSRQQLFKPIGDRGQEKIRNKHVLIVGAGALGSASAESFVRAGIGKLTIIDRDYVE
CCHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCHHH
WSNLQRQQLYSEEDAREKLPKAIAAKNRLEKLNSEVQIDAFVMDACAENLEGLLENVDVI
HHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
IDATDNFDIRFIINDLSQKYNIPWVYGSCVGSYGMSYTIIPQETPCLHCVLKNVPVTGVT
EECCCCCEEEEEEEHHHHHCCCCEEEHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEE
CDTAGIISPTVQIVAAYQVAEALKILVEDFAAIRKTFFMFDIWSNQNHFIKLGKIKTDDC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCEECCCC
PSCGLNRTYPYLSYENQTKVAVLCGRNTVQIRTVESRQYNFDDIEKVLKKLGEVDRNPYL
CCCCCCCCCCEEEECCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
LSCQLDEYRVVIFRDGRVFIHGTNDISKAKQLYYRVFG
EEEEECCEEEEEEECCEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]