Click here to switch to the map view.

The map label for this gene is yjeA

Identifier: 30065532

GI number: 30065532

Start: 4479223

End: 4480200

Strand: Direct

Name: yjeA

Synonym: S4578

Alternate gene names: 30065532

Gene position: 4479223-4480200 (Clockwise)

Preceding gene: 30065525

Following gene: 161486369

Centisome position: 97.39

GC content: 52.25

Gene sequence:

>978_bases
ATGAGCGAAACGGCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGATCCG
TCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGTATGAGCCAGGCGACGGTAACCGATATTCATTTGG
TCCCGTTTGAGACACGTTTCGTTGGCCCCGGGCATTCGCAGGGGATGAATCTCTGGTTAATGACCAGCCCGGAATACCAT
ATGAAACGCCTGCTGGTTGCTGGTTGTGGGCCGGTATTCCAGCTGTGCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTA
TCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATC
TCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGAC
CCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGA
CCGCGACACGTTGCTACAATTGCTGTTTACCTTTGGCGTAGAGCCAAATATTGGTAAAGAAAAACCGACCTTTGTGTACC
ACTTTCCAGCCAGCCAGGCATCACTGGCGCAAATCAGTACCGAAGATCATCGGGTCGCTGAACGCTTTGAGGTTTATTAT
AAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTAACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCG
TAAGCGCGCGGCGCGCGGTTTGCCGCAGCACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACT
GTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTGAAGTCATCGCCTTT
AGCGTTGACCGGGCATAA

Upstream 100 bases:

>100_bases
TTAGTCACTGCCCACCGCTGTTTGATTCCTGCGTAAAATTTGTCTCGCCGTCGCGTTGCGAGTAGACTTCGTGCCCTTGT
CAAAAACTGGAGATTTAACT

Downstream 100 bases:

>100_bases
TTCTGAAAAATTACTGAATTAACAGCGAAGAATGGCGTGATAAGCGCCATTCTGCAGCATATGTTTTCATTTTTCCGTTA
AAGGCCACAATTTAAGACTG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYH
MKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAF
SVDRA

Sequences:

>Translated_325_residues
MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYH
MKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAF
SVDRA
>Mature_324_residues
SETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHM
KRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEIDP
LSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYYK
GIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFS
VDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=338, Percent_Identity=27.2189349112426, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194272210, Length=338, Percent_Identity=27.2189349112426, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=100, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=327, Percent_Identity=31.4984709480122, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789256, Length=321, Percent_Identity=30.5295950155763, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994340, Length=340, Percent_Identity=30, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17535927, Length=340, Percent_Identity=30, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17535925, Length=340, Percent_Identity=30, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320242, Length=338, Percent_Identity=27.2189349112426, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324256, Length=347, Percent_Identity=27.6657060518732, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640849, Length=335, Percent_Identity=27.4626865671642, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24640851, Length=335, Percent_Identity=27.4626865671642, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36977; Mature: 36845

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCEE
VGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRP
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEIDPLSADKTQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHH
VADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHH
VDRLVMLALGAETLAEVIAFSVDRA
HHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCEE
VGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRP
CCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEIDPLSADKTQLREVAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHH
VADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYY
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHH
VDRLVMLALGAETLAEVIAFSVDRA
HHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA