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The map label for this gene is yjiE

Identifier: 30065416

GI number: 30065416

Start: 4356465

End: 4357376

Strand: Direct

Name: yjiE

Synonym: S4447

Alternate gene names: 30065416

Gene position: 4356465-4357376 (Clockwise)

Preceding gene: 30065415

Following gene: 30065423

Centisome position: 94.72

GC content: 51.21

Gene sequence:

>912_bases
ATGGATGACTGTGGTGCGGTTTTGCATAATATTGAAACCAAATGGCTTTATGATTTTCTGACTCTGGAAAAATGCCGCAA
TTTTTCCCAGGCGGCAGTCAGTCGCAACGTCTCGCAACCGGCATTCAGCCGCCGCATCCGTGCACTGGAACAGGCGATTG
GCGTTGAATTGTTTAACCGCCAGGTGACGCCACTGCAACTCTCAGAACAAGGGAAAATCTTCCATTCGCAGATCCGCCAT
CTGTTGCAACAGTTAGAAAGCAACCTTGCAGAGCTGCGTGGCGGCAGCGATTACGCGCAACGTAAAATCAAGATAGCCGC
TGCACACTCTCTTTCCCTCGGTCTGTTACCGTCCATTATCAGCCAGATGCCGCCGCTCTTTACCTGGGCAATTGAAGCCA
TTGATGTCGATGAAGCGGTCGATAAACTGCGTGAAGGGCAAAGTGACTGTATTTTTTCCTTTCACGACGAAGATTTGCTG
GAAGCGCCGTTTGACCACATTCGCTTATTTGAATCTCAATTGTTCCCTGTCTGCGCCAGTGACGAACACGGAGAAGCACT
TTTTAACCTCGCGCAGCCACACTTTCCGTTACTGAATTACAGCCGCAACTCCTACATGGTGCGATTGATTAATCGCACCC
TGACGCGCCACAGTGAGTTAAGTTTCAGCACCTTTTTTGTCTCTTCGATGAGCGAGCTTTTAAAGCAGGTTGCCCTCGAC
GGCTGTGGGATTGCCTGGCTGCCGGAGTATGCCATCCAACAAGAAATTCGCAGCGGGCAGCTCGTTGTGCTGAATCGGGA
CGAACAGGTGATCCCGATTCAGGCCTACGCATACCGGATGAATACCCGCATGAATCCAGTTGCCGAACGCTTCTGGCGTG
AACTGCGCGAGCTGGAGATTGTGCTTAGCTGA

Upstream 100 bases:

>100_bases
AAAGCCTGCGTGAAAGGAACGTTTGAAACGGCTTAAACGGTTGTCAGCCGCCCTAATGGACGTAGAATGCCCCCAAGGGC
GGCTGACAGAGTAAAACGTA

Downstream 100 bases:

>100_bases
CATTCTCAAGCGTCGCACTGCGCCAACCGCCATTATCCCAGACCAGATCAAACGTTAGCGGTTCATCGTCGTGGTACAGC
ATTATCTCAAGGCTGATCGC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: Quorum-sensing regulator protein D [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS

Sequences:

>Translated_303_residues
MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS
>Mature_303_residues
MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS

Specific function: Down-regulates expression of the flagellar regulon, decreasing motility [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790783, Length=303, Percent_Identity=98.6798679867987, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1787601, Length=280, Percent_Identity=23.9285714285714, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34754; Mature: 34754

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNR
CCCHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
QVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSII
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHHHHHHHHHH
SQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCAS
HHCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCCCC
DEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRELEI
CCCCHHCCHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VLS
HCC
>Mature Secondary Structure
MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNR
CCCHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
QVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSII
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHHHHHHHHHH
SQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCAS
HHCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCCCC
DEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAYAYRMNTRMNPVAERFWRELRELEI
CCCCHHCCHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VLS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7876157 [H]