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The map label for this gene is yhjM

Identifier: 30065188

GI number: 30065188

Start: 4114208

End: 4115314

Strand: Direct

Name: yhjM

Synonym: S4204

Alternate gene names: 30065188

Gene position: 4114208-4115314 (Clockwise)

Preceding gene: 30065187

Following gene: 229089131

Centisome position: 89.45

GC content: 54.11

Gene sequence:

>1107_bases
ATGAATGTGTTGCGTAGTGGAATCGTGACGATGCTGTTGTTGGCTGCCTTTAGTGTTCAGGCAGCCTGTACCTGGCCTGC
CTGGGAGCAGTTTAAAAAGGATTACATCAGTCAGGAAGGGCGCGTCATCGACCCCAGCGACGCGCGCAAAATCACCACCT
CCGAAGGGCAAAGTTACGGTATGTTCTTTGCCCTGGCGGCTAACGACCGTGCAGCTTTCGATAATATTCTCGACTGGACG
CAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCCTGGCTGTGGGGCAAGAAAGAGAACAGTAAGTGGGA
AGTGCTGGACAGCAATTCGGCCTCCGATGGTGATGTCTGGATGGCCTGGTCGTTGCTGGAGGCGGGGCGTTTGTGGAAAG
AGCAGCGTTATACCGACATCGGCAGCGCGTTGCTAAAACGTATCGCGCGGGAGGAAGTGGTGACGGTGCCTGGGCTGGGT
TCCATGTTGTTACCGGGCAAAGTGGGTTTTGCTGAGGATAACAGCTGGCGTTTTAACCCCAGCTACATGCCGCCGACGCT
GGCGCAGTATTTCACCCGCTTTGGCGCGCCGTGGACCACGCTGCGCGAAACCAATCAACATTTATTGCTGGAAACCGCCC
CGAAAGGCTTTTCGCCAGACTGGGTGCGCTATGAGAAAGACAAAGGCTGGCAGCTAAAAGCCGAAAAAACATTGATCAGC
AGCTACGACGCTATCCGCGTTTACATGTGGGTAGGCATGATGCCTGACAGCGATCCGCAAAAAGCGCGGATGCTCAACCG
GTTTAAACCGATGGCGACATTCACTGAGAAAAACGGTTATCCGCCGGAAAAAGTGGATGTGGCTACGGGGAAAGCGCAGG
GTAAAGGACCGGTCGGTTTTTCTGCCGCCATGCTGCCTTTTTTACAAAACCGCGATGCGCAGGCAGTTCAGCGCCAGCGC
GTGGCCGATAACTTTCCCGGCAGCGATACCTATTACAACTATGTGCTGACCCTGTTTGGACAAGGCTGGGATCAACACCG
TTTCCGCTTCTCGACAAAAGGTGAGTTATTACCTGACTGGGGCCAGGAATGCGCAAATTCACACTAA

Upstream 100 bases:

>100_bases
GCTGGCGACTATCAGTGTGATATTGCTGGCATGGGTACTGTGGCGTCTGCTGCGAATTATTAGTCGTCGTCGTCTTAACC
CGGATAACGAGTAATTGAAG

Downstream 100 bases:

>100_bases
ACATATTCACGCTTTCCCTCGGTCTGGCCGTCATGCCGATGGTCGAGGCAGCACCAACCATCAGCAGACCCTGCGGATGG
ACAGCGGCAACACTAATGCC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILDWT
QNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLIS
SYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQR
VADNFPGSDTYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH

Sequences:

>Translated_368_residues
MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILDWT
QNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLIS
SYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQR
VADNFPGSDTYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH
>Mature_368_residues
MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILDWT
QNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLIS
SYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQR
VADNFPGSDTYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=368, Percent_Identity=98.9130434782609, Blast_Score=753, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41790; Mature: 41790

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYG
CCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCC
MFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVW
EEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCCCEEEEECCCCCCCCEE
MAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEECC
SYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLIS
CCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEHHHHHHH
SYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGF
HHHHEEEEEEEECCCCCCHHHHHHHHHHCCHHHECCCCCCCCCCEEECCCCCCCCCCCCH
SAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNYVLTLFGQGWDQHRFRFSTKGELLPDW
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHH
GQECANSH
HHHHCCCC
>Mature Secondary Structure
MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYG
CCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCC
MFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVW
EEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCCCEEEEECCCCCCCCEE
MAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEECC
SYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLIS
CCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEHHHHHHH
SYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGF
HHHHEEEEEEEECCCCCCHHHHHHHHHHCCHHHECCCCCCCCCCEEECCCCCCCCCCCCH
SAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNYVLTLFGQGWDQHRFRFSTKGELLPDW
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHH
GQECANSH
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]