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The map label for this gene is yiaJ

Identifier: 30065141

GI number: 30065141

Start: 4051111

End: 4051959

Strand: Direct

Name: yiaJ

Synonym: S4151

Alternate gene names: 30065141

Gene position: 4051111-4051959 (Clockwise)

Preceding gene: 30065134

Following gene: 30065142

Centisome position: 88.08

GC content: 52.18

Gene sequence:

>849_bases
ATGGGGAAAGAAGTGATGGGAAAAAAAGAGAACGAGATGGCGCAGGAAAAAGAGCGTCCAGCCGGAAGCCAGAGTCTGTT
TCGCGGGTTGATGCTGATTGAGATTTTGAGCAACTATCCAAACGGTTGTCCGTTGGCGCATCTTTCGGAGCTGGCTGGTT
TAAATAAGAGTACCGTCCATCGCTTATTGCAGGGATTACAGTCCTGCGGCTATGTGACCACCGCGCCCGCCGCAGGGAGT
TACCGCCTGACTACCAAATTTATTGCCGTCGGGCAGAAGGCGCTGTCTTCGCTGAATATCATTCATATCGCCGCTCCGCA
TCTTGAGGCACTGAACATCGCCACTGGTGAAACCATTAACTTCTCCAGCCGCGAAGACGATCACGCTATTTTGATTTATA
AGCTGGAACCCACAACCGGGATGCTGCGAACCCGTGCCTATATTGGCCAGCATATGCCGCTCTACTGTTCCGCAATGGGC
AAGATCTACATGGCGTTTGGTCACCCGGACTACGTGAAGTCATACTGGGAAAACCATCAGCATGAGATCCAGCCGTTAAC
CCGCAATACCATTACCGAGCTGCCCGCGATGTTCGACGAACTGGCGCACATTCGTGAAAGCGGAGCGGCGATGGACAGAG
AAGAAAACGAACTCGGCGTCTCCTGTATTGCTGTTCCGGTGTTTGATATTCATGGGCGGGTGCCGTACGCCGTGTCGATT
TCGCTTTCGACATCACGTCTGAAACAGGTGGGAGAGAAAAATCTCCTGAAACCACTGCGTGAAACCGCGCAGGCTATTTC
TAATGAACTGGGATTTACTGTCAGGGATGACCTGGGCGCGATCACATAA

Upstream 100 bases:

>100_bases
ATTGATGAATTTCAAAAGACATAAAAATCAAGTGGTTATAAATTATCTGTCGAGATCGTGAACTACGGCACACTTTGCGC
TACCATCAGGACGCGACAAA

Downstream 100 bases:

>100_bases
CGCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTG
TTTTAGTGCAAAGGAATGAT

Product: putative regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGS
YRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDLGAIT

Sequences:

>Translated_282_residues
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGS
YRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDLGAIT
>Mature_281_residues
GKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSY
RLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGK
IYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSIS
LSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDLGAIT

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=282, Percent_Identity=99.645390070922, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.4661354581673, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=34.2105263157895, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=29.8804780876494, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790752, Length=219, Percent_Identity=30.5936073059361, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788581, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693096, Length=217, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31094; Mature: 30963

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVH
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
RLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETIN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEEC
FSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWENHQ
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHCCH
HEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEE
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDLGAIT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
>Mature Secondary Structure 
GKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVH
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
RLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETIN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEEC
FSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWENHQ
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHCCH
HEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEE
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDLGAIT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]