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The map label for this gene is lldD

Identifier: 30065119

GI number: 30065119

Start: 4021602

End: 4022792

Strand: Reverse

Name: lldD

Synonym: S4124

Alternate gene names: 30065119

Gene position: 4022792-4021602 (Counterclockwise)

Preceding gene: 30065120

Following gene: 30065118

Centisome position: 87.46

GC content: 55.67

Gene sequence:

>1191_bases
ATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGG
TGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCACTGCGCCAGCGTATTCTGAAAA
ACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTAGCACTGGCACCGGTGGGTTTG
TGTGGCATGTATGCGCGTCGTGGCGAAGTTCAGGCAGCCAAAGCGGCGGACGCGCATGGCATTCCGTTTACTCTCTCGAC
GGTTTCCGTTTGCCCGATTGAAGAAGTCGCGCCAGCCATCAAGCGCCCAATGTGGTTCCAGCTTTATGTACTGCGCGATC
GCGGCTTTATGCGTAACGCGCTGGAGCGAGCAAAAGCAGCGGGTTGTTCGACGCTGGTTTTCACCGTGGATATGCCGACA
CCGGGCGCACGCTACCGTGATGCGCATTCAGGTATGAGCGGCCCAAATGCGGCAATGCGCCGCTACTTGCAGGCGGTGAC
GCATCCGCAATGGGCGTGGGATGTGGGCCTGAACGGTCGTCCACATGATTTAGGTAATATCTCAGCTTATCTCGGCAAAC
CGACCGGACTGGAAGATTACATCGGCTGGCTGGGGAATAACTTCGATCCGTCCATCTCATGGAAAGACCTTGAGTGGATC
CGCGATTTCTGGGATGGCCCGATGGTGATCAAAGGGATCCTCGATCCGGAAGATGCGCGCGATGCAGTACGTTTTGGTGC
TGATGGAATTGTGGTTTCTAACCACGGTGGCCGCCAGCTGGACGGTGTACTCTCTTCCGCTCGTGCGTTGCCCGCTATTG
CAGATGCGGTGAAAGGTGATATCGCCATTCTGGCGGATAGCGGAATTCGTAACGGGCTTGATGTCGTGCGTATGATTGCG
CTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCCTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCT
GCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGTGAAATTACGCAAGATT
CGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTACGGCACTGGCTCCGATGGCGAAAGGGAATGCGGCATAG

Upstream 100 bases:

>100_bases
TTCACACCACCATGAAACGATTCGATGAAGATCAGGCTCGCCACGCACGGATTACCCGCCTGCCCGGTGAGCATAATGAG
CATTCGAGGGAGAAAAACGC

Downstream 100 bases:

>100_bases
TCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAA
ACGACACCGCACTCTTTTTT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGL
CGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWI
RDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIA
LGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAKGNAA

Sequences:

>Translated_396_residues
MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGL
CGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWI
RDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIA
LGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAKGNAA
>Mature_396_residues
MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGL
CGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWI
RDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIA
LGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAKGNAA

Specific function: Unknown

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain

Homologues:

Organism=Homo sapiens, GI11068137, Length=367, Percent_Identity=33.5149863760218, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI54234014, Length=378, Percent_Identity=34.3915343915344, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI7705393, Length=378, Percent_Identity=34.3915343915344, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1790033, Length=396, Percent_Identity=99.7474747474748, Blast_Score=805, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193208036, Length=371, Percent_Identity=34.5013477088949, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI32566217, Length=284, Percent_Identity=32.3943661971831, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323587, Length=382, Percent_Identity=29.0575916230367, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI78707188, Length=378, Percent_Identity=34.1269841269841, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI281363140, Length=378, Percent_Identity=34.1269841269841, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI78707190, Length=378, Percent_Identity=34.1269841269841, Blast_Score=208, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LLDD_ECO24 (A7ZTF9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465088.1
- ProteinModelPortal:   A7ZTF9
- SMR:   A7ZTF9
- STRING:   A7ZTF9
- EnsemblBacteria:   EBESCT00000020166
- GeneID:   5587517
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4109
- eggNOG:   COG1304
- GeneTree:   EBGT00050000009653
- HOGENOM:   HBG517781
- OMA:   WIREFWE
- ProtClustDB:   PRK11197
- BioCyc:   ECOL331111:ECE24377A_4109-MONOMER
- HAMAP:   MF_01559
- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259
- InterPro:   IPR020920
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000138

Pfam domain/function: PF01070 FMN_dh

EC number: =1.1.2.3

Molecular weight: Translated: 42759; Mature: 42759

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH_1; PS51349 FMN_HYDROXY_ACID_DH_2

Important sites: ACT_SITE 275-275 BINDING 24-24 BINDING 106-106 BINDING 127-127 BINDING 129-129 BINDING 155-155 BINDING 164-164 BINDING 251-251 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSL
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
ETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAI
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEECCEEECCHHHHHHHH
KRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPTPGARYRDAHSGMSGPNAAMR
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHCCCCCCHHHHH
RYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWI
HHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
RDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGD
HHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
IAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMT
EEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LTGAKSISEITQDSLVQGLGKELPTALAPMAKGNAA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSL
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
ETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAI
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEECCEEECCHHHHHHHH
KRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPTPGARYRDAHSGMSGPNAAMR
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHCCCCCCHHHHH
RYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWI
HHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
RDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGD
HHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
IAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMT
EEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LTGAKSISEITQDSLVQGLGKELPTALAPMAKGNAA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA