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The map label for this gene is intS [H]

Identifier: 30065067

GI number: 30065067

Start: 3967276

End: 3968487

Strand: Reverse

Name: intS [H]

Synonym: S4835

Alternate gene names: 30065067

Gene position: 3968487-3967276 (Counterclockwise)

Preceding gene: 30065069

Following gene: 30065066

Centisome position: 86.28

GC content: 44.97

Gene sequence:

>1212_bases
ATGAATTCATCAAAAGCCGGAGGATGTCATGGCATTAACGATATCAAAGTGAAAACCGCAAAACCCAAAGACAAACCCTA
TAAGCTTGCTGATGGCGGCGGTATGTATCTACTGATTAACACTAATGGTTCTAAATACTGGCGCATGAAGTACCGCTTTG
CTGGTAAAGAAAAGATGCTTTCTATTGGCGTATACCCCGATGTAACGCTAGCCGATGCTCGCGAGAAACGCAGTGAAGCC
AGGAAACTCCTCGCTGCTGGAGGCGATCCAGGGGAGGCAAAAAAGGAAGAAAAAATCGCGCAGCAAATGAGTCTGAAAAA
TACGTTCGAGGCTATTGCTCGCGAATGGCACCAGTTAAAAGCTGATCGGTGGTCATTACGCTATCGTGATGAAATCATTG
ATACCTTTGAAAAAGACATTTTTCCCTACATTGGCAAGCGCCCTATTGCCGAAATCAAACCAATGAAGTTACTGGAAGCA
CTGCGTAAAATGGAAAAACGTGGAGCTCTGGAGAAAATGCGCAAGGTCCGCCAGCGCTGTGGTGAGGTGTTCCGCTATGC
AATTGTTACTGGCCGGGCAGACTATAACCCAGCCCCCGATCTTGCCAGTGCTTTAGCTACACCGAAAAAAGTACATTTTC
CCTTTCTTACTGCTAATGAACTTCCCCACTTCCTCAACGATCTGGCGGGTTATACCGGAAGTATAATCACTAAGACAGCT
ACTCAGATCATTATGTTAACCGGCGTACGAACACAAGAACTGCGTTTTGCACGCTGGGAAGATATCGATTTTGAGACAAA
ATTGTGGGAGATCCCGGCAGAAGTAATGAAGATGAAGCGACCTCATATTGTGCCGCTCTCTGAGCAGGTCATTATGCTAT
TTAAGCAGCTTGAACCAATCTCAAAACATCATCCCCTAGTCTTTATCGGTAGAAATGATCCCCGCAAACCAATTAGTAAG
GAAAGCATTAACCAAGTAATTGAATTACTTGGATATAAGGGAAGACTCACAGGGCACGGTTTCAGACACACCATGAGTAC
AATTCTGCATGAACAAGGCTTTAATTCCGCCTGGATTGAAATGCAGTTAGCTCATGTGGATAAAAATTCCATCAGGGGTA
CCTATAATCATGCCCTGTATCTCGATGGCCGCCGTGAAATGATGCAATGGTACGCTGATTACATTGATTCACTCTCCAGC
CGGGAAAGTTAA

Upstream 100 bases:

>100_bases
AAATGTGATACATAACAACGGCTTTTAACAATATCTTTGTTATACAAGGGGTGTATTGGGGGTATCAGGTTAGTTACTGG
CAGAAATCCCCCTCAGGAAC

Downstream 100 bases:

>100_bases
GTCAGAAATACTTTTCCCTGATGTTTTCCTCAATTAAGTATTTTATCCTCTGGCCTACAAGGCTGGAGGATTTTTGTTAT
TATATTATTTTTTTGCGTCT

Product: putative integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEA
RKLLAAGGDPGEAKKEEKIAQQMSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTA
TQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISK
ESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSS
RES

Sequences:

>Translated_403_residues
MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEA
RKLLAAGGDPGEAKKEEKIAQQMSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTA
TQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISK
ESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSS
RES
>Mature_403_residues
MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEA
RKLLAAGGDPGEAKKEEKIAQQMSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTA
TQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISK
ESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSS
RES

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=382, Percent_Identity=48.6910994764398, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI145693166, Length=385, Percent_Identity=42.5974025974026, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1788974, Length=391, Percent_Identity=39.1304347826087, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46314; Mature: 46314

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKML
CCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCCCEE
SIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEAIAREWHQLK
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGALEKMRKVRQRC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTA
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHHHHHCCCCCHHHHHHH
TQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPI
HHEEEEECCCHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCH
SKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIE
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHEE
MQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES
EEEECCCCHHCCCEECEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRFAGKEKML
CCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCCCEE
SIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEAIAREWHQLK
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGALEKMRKVRQRC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLNDLAGYTGSIITKTA
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHHHHHCCCCCHHHHHHH
TQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVPLSEQVIMLFKQLEPI
HHEEEEECCCHHHHHHHCCCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCH
SKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIE
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHEE
MQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES
EEEECCCCHHCCCEECEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]