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The map label for this gene is yieH

Identifier: 30065026

GI number: 30065026

Start: 3920652

End: 3921308

Strand: Direct

Name: yieH

Synonym: S4027

Alternate gene names: 30065026

Gene position: 3920652-3921308 (Clockwise)

Preceding gene: 30065024

Following gene: 30065027

Centisome position: 85.24

GC content: 48.55

Gene sequence:

>657_bases
ATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCCCGCGCATATGT
AACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAA
TTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCT
CGGCTGTTCGATTCAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGG
CCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACG
ATATTCAGCGCTGGAAGCCAGACCCGGCGTTAATGTTCCATGCGGCAAAAGCGATGAATGTAAATGTAGAAAACTGCATT
CTGGTTGATGACTCAGTTGCCGGTGCGCAATCTGGTATCGACGCAGGTATGGAAGTGTTCTACTTCTGCGCCGACCCGCA
CAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCGGAACTGTGGAAAGCGCGTGGTT
GGGATATTACGGCATAG

Upstream 100 bases:

>100_bases
CGTTTGTGAGAGACTTTTTTATTGGATTTACTTATACGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAA
ACTGATTAAAAAGGAAAGGC

Downstream 100 bases:

>100_bases
TTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACG
TCGGGTAATACATCACGGAC

Product: 6-phosphogluconate phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVA
RLFDSAIEGAGALLSAITAPMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA

Sequences:

>Translated_218_residues
MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVA
RLFDSAIEGAGALLSAITAPMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
>Mature_217_residues
SRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVAR
LFDSAIEGAGALLSAITAPMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCIL
VDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=221, Percent_Identity=98.6425339366516, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI87082080, Length=202, Percent_Identity=30.1980198019802, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24293; Mature: 24162

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFKGVKLYEIIDIVSL
CCCCEEEEEECCCCEECHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHHHHHHHH
EHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMCVVSNGPNNKMQHSMGKLN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
MLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCILVDDSVAGAQSGIDAGMEVF
HHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCEEE
YFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFKRFKGVKLYEIIDIVSL
CCCEEEEEECCCCEECHHHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCHHHHHHHHHH
EHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMCVVSNGPNNKMQHSMGKLN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
MLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCILVDDSVAGAQSGIDAGMEVF
HHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCEEE
YFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]