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The map label for this gene is glvB

Identifier: 30064993

GI number: 30064993

Start: 3883802

End: 3884287

Strand: Reverse

Name: glvB

Synonym: S3989

Alternate gene names: 30064993

Gene position: 3884287-3883802 (Counterclockwise)

Preceding gene: 30064995

Following gene: 30064991

Centisome position: 84.45

GC content: 50.62

Gene sequence:

>486_bases
ATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCATTGGGTTGTGCTTTACCCTGCTGTACTTCGTGGTTTT
CCGCACCCTGATTCTGCAGTTCAACATGTGCACGCCGGGACGTGAAGATGCGGAAGTGAAACTCTACTCAAAAGCCGAAT
ACAAAGCCTCGCGAGGCCAAACCACCGCTGCAGAGCCAAAAAAAGAGCTGGATCAGGCTGCCGGTATCCTGCAAGCCCTG
GGCGGGGTCGGCAATATCTCCAGCATTAACAATTGCGCGACGCGTCTACGTATTGCACTGCATGACATGTCACAAACGCT
GGATGACGAAGTCTTTAAAAAGCTGGGAGCGCACGGCGTCTTCCGTAGTGGCGATGCCATTCAGGTGATCATTGGTCTGC
ATGTATCCCAATTGCGTGAACAGCTCGATAGCTTAATTAATTCTCATCAATCAGCAGAAAATGTTGCCATTACGGAGGCA
GTATAA

Upstream 100 bases:

>100_bases
GCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACCAGGTTT
TACCGCAAAACTGGATCCCG

Downstream 100 bases:

>100_bases
TGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAG
GACCGTTTCCCGCTTCGTGC

Product: PTS system arbutin-specific transporter subunit IIB

Products: NA

Alternate protein names: PTS system EIIB component

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQAL
GGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEA
V

Sequences:

>Translated_161_residues
MFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQAL
GGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEA
V
>Mature_161_residues
MFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQAAGILQAL
GGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEA
V

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1264

COG function: function code G; Phosphotransferase system IIB components

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-1 domain

Homologues:

Organism=Escherichia coli, GI1787343, Length=146, Percent_Identity=32.1917808219178, Blast_Score=83, Evalue=7e-18,
Organism=Escherichia coli, GI1787908, Length=134, Percent_Identity=33.5820895522388, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1786894, Length=136, Percent_Identity=33.0882352941176, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTXB_ECOLI (P69789)

Other databases:

- EMBL:   L10328
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65170
- RefSeq:   AP_004111.1
- ProteinModelPortal:   P69789
- SMR:   P69789
- EnsemblBacteria:   EBESCT00000002463
- EnsemblBacteria:   EBESCT00000014671
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3659
- EchoBASE:   EB1660
- EcoGene:   EG11709
- eggNOG:   COG1263
- GeneTree:   EBGT00050000009159
- HOGENOM:   HBG086560
- BioCyc:   EcoCyc:GLVB-MONOMER
- Genevestigator:   P69789
- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR011535
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826

Pfam domain/function: PF00367 PTS_EIIB; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 17626; Mature: 17626

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS

Important sites: ACT_SITE 92-92

Signals:

None

Transmembrane regions:

HASH(0x160634d4)-;

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCC
TTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
>Mature Secondary Structure
MFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCC
TTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503; 8019415