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The map label for this gene is uhpA

Identifier: 30064982

GI number: 30064982

Start: 3872000

End: 3872590

Strand: Reverse

Name: uhpA

Synonym: S3976

Alternate gene names: 30064982

Gene position: 3872590-3872000 (Counterclockwise)

Preceding gene: 30064983

Following gene: 30064981

Centisome position: 84.2

GC content: 59.05

Gene sequence:

>591_bases
ATGATCACCGTTGCCCTTATAGACGATCACCTCATCGTCCGCTCCGGCTTTGCGCAGCTGCTGGGGCTGGAACCTGATTT
GCAGGTAGTTGCCGAGTTTGGTTCGGGGCGCGAGGCGCTGGCGGGGCTGCCGGGGCGCGGTGTGCAGGTGTGTATTTGCG
ATATCTCCATGCCCGATATCTCCGGTCTGGAGCTGCTAAGCCAGCTGCCGAAAGGTATGGCGACGATTATGCTCTCCGTT
CACGACAGTCCTGCGCTGGTTGAGCAGGCGCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGA
ACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATCCGGTC
GTCAGGACCCGCTGACCAAACGTGAACGCCAGGTGGCGGAAAAACTGGCGCAAGGAATGGCGGTGAAAGAGATTGCCGCC
GAACTGGGCTTGTCACCGAAAACGGTACACGTCCATCGCGCCAATCTGATGGAAAAACTGGGCGTCAGTAACGACGTAGA
GCTGGCGCGCCGCATGTTTGATGGCTGGTGA

Upstream 100 bases:

>100_bases
ACAAGATCGCGGTGTTTTTTCAGTAACCGCTCAAGGCTTGAACAACATCGCGCTTATCGTTAAGGTAAGCGCGTTTTTTT
TTACCCGCCAGGACAAGACC

Downstream 100 bases:

>100_bases
TGAAGACGTTGTTCTCCCGCTTAATTACCGTTATTGCCTGCTTTTTTATCTTCTCTGCCGCATGGTTTTGCCTATGGAGT
ATCAGCCTGCATCTGGTTGA

Product: DNA-binding transcriptional activator UhpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDISGLELLSQLPKGMATIMLSV
HDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW

Sequences:

>Translated_196_residues
MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDISGLELLSQLPKGMATIMLSV
HDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW
>Mature_196_residues
MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDISGLELLSQLPKGMATIMLSV
HDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW

Specific function: Appears to be the positive activator of uhpT transcription

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=100, Blast_Score=396, Evalue=1e-112,
Organism=Escherichia coli, GI1788222, Length=200, Percent_Identity=36.5, Blast_Score=125, Evalue=1e-30,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=36.0824742268041, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=33.879781420765, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=29.5918367346939, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788712, Length=183, Percent_Identity=28.9617486338798, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788546, Length=203, Percent_Identity=29.5566502463054, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UHPA_ECO57 (P0AGA8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91204
- PIR:   H86050
- RefSeq:   NP_290304.1
- RefSeq:   NP_312633.1
- ProteinModelPortal:   P0AGA8
- SMR:   P0AGA8
- EnsemblBacteria:   EBESCT00000025868
- EnsemblBacteria:   EBESCT00000058328
- GeneID:   915428
- GeneID:   960804
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5159
- KEGG:   ecs:ECs4606
- GeneTree:   EBGT00050000008645
- HOGENOM:   HBG753323
- OMA:   NVELAHR
- ProtClustDB:   PRK10360
- BioCyc:   ECOL83334:ECS4606-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 20890; Mature: 20890

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDI
CEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCC
SGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGC
HHHHHHHHCCCHHHHEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCE
YLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAAELGLSPKTVHVHRANLMEKL
EECCHHEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEHHHHHHHHHH
GVSNDVELARRMFDGW
CCCCHHHHHHHHHCCC
>Mature Secondary Structure
MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDI
CEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCC
SGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATGGC
HHHHHHHHCCCHHHHEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCE
YLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAAELGLSPKTVHVHRANLMEKL
EECCHHEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEHHHHHHHHHH
GVSNDVELARRMFDGW
CCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796