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The map label for this gene is rbsR

Identifier: 30064941

GI number: 30064941

Start: 3824594

End: 3825577

Strand: Reverse

Name: rbsR

Synonym: S3931

Alternate gene names: 30064941

Gene position: 3825577-3824594 (Counterclockwise)

Preceding gene: 30064942

Following gene: 30064937

Centisome position: 83.18

GC content: 50.41

Gene sequence:

>984_bases
ATGAAAGATGTTGCCCGTTTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGA
AGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCA
ATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGC
AGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAAC
GCTGATGCAAAAACGCGTTGATGGCTTACTGTTACTGTGTACCGAAACGCATCAGCCTTCGCGTGAAATCATGCAACGTT
ATCCGACAGTGCCTACCGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAACTCGTTGCTG
GGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGTCCGCTGGATAAAAC
TCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCA
CTGGTGATTTTGAATTTAACGGCGGGTTTGACGCCATGCGCCAACTGTTATCACATCCGCTGCGTCCTCAGGCCGTCTTT
ACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGT
GATTGGCTATGACGATATCGAACTGGCAAGCTTTATGACGCCACCATTAACCACTATCCACCAACCGAAAGATGAACTGG
GGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATT
CTGATGGAACGCGGTTCGGCATAG

Upstream 100 bases:

>100_bases
CGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGC
AGAGGTGACGCTTGGCTACA

Downstream 100 bases:

>100_bases
ATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATA
ATGCCCATCGTGATAAACGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLL
GGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVF
TGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPI
LMERGSA

Sequences:

>Translated_327_residues
MKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLL
GGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVF
TGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPI
LMERGSA
>Mature_327_residues
MKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLL
GGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVF
TGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPI
LMERGSA

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790194, Length=327, Percent_Identity=100, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=327, Percent_Identity=46.4831804281346, Blast_Score=316, Evalue=1e-87,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=35.1791530944625, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=32.7586206896552, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=30.379746835443, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1786540, Length=309, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=29.1390728476821, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=27.3026315789474, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=28.7461773700306, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786268, Length=323, Percent_Identity=28.1733746130031, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=25.6172839506173, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=26.3843648208469, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789456, Length=314, Percent_Identity=26.1146496815287, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790192, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=23.9263803680982, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBSR_ECO57 (P0ACQ2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91215
- PIR:   H86061
- RefSeq:   NP_290392.1
- RefSeq:   NP_312722.1
- ProteinModelPortal:   P0ACQ2
- SMR:   P0ACQ2
- EnsemblBacteria:   EBESCT00000027373
- EnsemblBacteria:   EBESCT00000058050
- GeneID:   915322
- GeneID:   960413
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5254
- KEGG:   ecs:ECs4695
- GeneTree:   EBGT00050000009237
- HOGENOM:   HBG753640
- OMA:   IMQRYPS
- ProtClustDB:   PRK10423
- BioCyc:   ECOL83334:ECS4695-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36309; Mature: 36309

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
MLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLC
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEEEEE
TETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRIACI
ECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
TGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVF
ECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEE
TGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDV
ECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LIHRITQPTLQQQRLQLTPILMERGSA
HHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
MLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLC
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEEEEE
TETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRIACI
ECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
TGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVF
ECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEE
TGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDV
ECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LIHRITQPTLQQQRLQLTPILMERGSA
HHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796