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The map label for this gene is basR

Identifier: 30064682

GI number: 30064682

Start: 3496896

End: 3497564

Strand: Reverse

Name: basR

Synonym: S3620

Alternate gene names: 30064682

Gene position: 3497564-3496896 (Counterclockwise)

Preceding gene: 30064683

Following gene: 30064681

Centisome position: 76.04

GC content: 53.21

Gene sequence:

>669_bases
ATGAAAATTCTGATTGTTGAAGACGATACGCTGTTATTGCAGGGACTGATTCTGGCGGCGCAAACCGAAGGCTACGCGTG
CGATGGCGTGACAACCGCGCGGATGGCGGAACAAAGCCTTGAGGACGGTCATTACAGCCTGGTGGTACTGGATTTAGGGT
TACCCGACGAAGATGGACTGCATTTTCTCGCCCGTATCCGGCAGAAAAAATACACCCTGCCGGTACTGATCCTCACCGCT
CGCGATACGCTGACCGACAAAATCGCCGGGCTGGATGTCGGTGCCGATGACTATCTGGTGAAGCCTTTTGCGCTGGAAGA
GTTACACGCCCGTATCCGCGCCCTGCTACGACGCCATAATAATCAGGGCGAAAGTGAGCTGATTGTTGGCAATCTGACGC
TGAATATGGGTCGCCGTCAGGTATGGATGGGCGGTGAAGAGTTGATTCTGACGCCCAAAGAATATGCTCTGCTGTCACGG
TTAATGCTCAAAGCAGGCAGTCCGGTACATCGGGAAATTCTCTACAACGACATCTATAACTGGGACAATGAACCCTCGAC
CAACACCCTGGAAGTGCATATCCACAATCTGCGCGACAAAGTGGGCAAAGCCCGTATCCGCACCGTGCGCGGCTTTGGCT
ATATGCTGGTCGCGAATGAGGAAAACTAA

Upstream 100 bases:

>100_bases
AAGACAATTTATTCTCCACGCTATTGGGATTAACTGGCGTTGAGACGAAGTATTACCAGGCCGCGGATGATATTCTGCAA
ACTTGCAGGAGAGTGAGTAA

Downstream 100 bases:

>100_bases
TTGAATCTGATGCGTTTTCTGCGCCGACCTATATCGCTGCGCCAACGGCTGATATTGACCATCGGGGCCATTTTGTTGGT
GTTTGAGCTGATCAGCGTCT

Product: DNA-binding transcriptional regulator BasR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTA
RDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN

Sequences:

>Translated_222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTA
RDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN
>Mature_222_residues
MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTA
RDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN

Specific function: Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=99.0990990990991, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=46.2962962962963, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=37.8378378378378, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=34.5454545454545, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=35.2173913043478, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36.4444444444444, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=35.5855855855856, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=37.5, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=32.7272727272727, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=34.2222222222222, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=32, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=30.6666666666667, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=30.3418803418803, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=27.0386266094421, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25045; Mature: 25045

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGL
CEEEEEECCHHHHHHHHEEEECCCEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
HFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHN
HHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHCC
NQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSRLMLKAGSPVHREILYNDIYN
CCCCCEEEEEEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
WDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN
CCCCCCCCEEEEEEEHHHHHHHHHHEEEEECCEEEEEEECCC
>Mature Secondary Structure
MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGL
CEEEEEECCHHHHHHHHEEEECCCEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
HFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHN
HHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHCC
NQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSRLMLKAGSPVHREILYNDIYN
CCCCCEEEEEEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
WDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN
CCCCCCCCEEEEEEEHHHHHHHHHHEEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]