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The map label for this gene is agaF

Identifier: 30064473

GI number: 30064473

Start: 3264042

End: 3264476

Strand: Direct

Name: agaF

Synonym: S3386

Alternate gene names: 30064473

Gene position: 3264042-3264476 (Clockwise)

Preceding gene: 30064472

Following gene: 30064474

Centisome position: 70.97

GC content: 53.1

Gene sequence:

>435_bases
ATGTTAAGTATTATTTTGACAGGGCATGGCGGGTTTGCCAGTGGCATGGAAAAAGCAATGAAGCAAATCCTCGGCGATCA
ATCGCAGTTTATTGCCATCGATTTTCCCGAAACCTCAAGCGCCGCGCTGCTTACATCGCAGCTTGAAGAGGCCATCGCCC
AACTGGATTGTGAAGACGGCATTGTTTTTCTGACGGATTTGCTGGGCGGCACGCCGTTTCGCGTAGCTTCAACGCTGGCG
ATGCAAAAACCGGGCTGTGAAGTGATTACCGGCACCAATTTGCAACTGCTACTGGAGATGGTGCTGGAGCGCGAAGGGTT
AAGCGGTGAAGAATTCCGTGTACAGGCGCTGGAGTGTGGACACCGAGGGCTGACCAGTCTGGTGGACGAGTTAGGCCGCT
GCCATGAAGAATGTCCGGTCGAGGAAGGAATATGA

Upstream 100 bases:

>100_bases
TACGCCGCGGCTAGAGTCCGCTGCGCCTGATTGCGGTTACCGTGGTTCTAGGGATCGTCGGTAAATTCTGCCATTTCCTT
TAAATACAATGAGGTATTAG

Downstream 100 bases:

>100_bases
CACACGTTCTGCGCGCCAGAAGGCTGCTGACTGAAGAGGGATGGTTCGATGACCATCAGTTGCGTGTTGCTGACGGTGTC
ATCGCAGCAATCGAACCGAT

Product: putative transport enzyme subunit

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLA
MQKPGCEVITGTNLQLLLEMVLEREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI

Sequences:

>Translated_144_residues
MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLA
MQKPGCEVITGTNLQLLLEMVLEREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI
>Mature_144_residues
MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLA
MQKPGCEVITGTNLQLLLEMVLEREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=130, Percent_Identity=33.0769230769231, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1788120, Length=127, Percent_Identity=33.0708661417323, Blast_Score=76, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15469; Mature: 15469

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQLDCEDG
CEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
IVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVLEREGLSGEEFRVQALECG
EEHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
HRGLTSLVDELGRCHEECPVEEGI
CCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQLDCEDG
CEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
IVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVLEREGLSGEEFRVQALECG
EEHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
HRGLTSLVDELGRCHEECPVEEGI
CCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]