Click here to switch to the map view.

The map label for this gene is yhaJ

Identifier: 30064449

GI number: 30064449

Start: 3235863

End: 3236759

Strand: Reverse

Name: yhaJ

Synonym: S3356

Alternate gene names: 30064449

Gene position: 3236759-3235863 (Counterclockwise)

Preceding gene: 30064452

Following gene: 30064438

Centisome position: 70.37

GC content: 55.74

Gene sequence:

>897_bases
ATGGCCAAAGAAAGGGCATTAACGCTGGAAGCACTACGGGTTATGGATGCGATCGATCGCCGGGGCAGTTTTGCGGCGGC
AGCGGATGAGCTGGGACGCGTGCCTTCCGCACTTAGCTACACCATGCAAAAACTGGAAGAAGAGCTGGATGTGGTGCTGT
TTGACCGCTCGGGCCATCGCACCAAATTCACCAATGTCGGGAGGATGTTGCTGGAGCGAGGGCGCGTTTTGCTGGAAGCC
GCAGATAAACTGACTACTGATGCGGAAGCTCTCGCGCGCGGTTGGGAAACGCATCTCACCATTGTGACCGAAGCGCTGGT
ACCGACACCTGCCTTTTTCCCGTTAATCGACAAACTGGCGGCAAAAGCCAATACCCAACTGGCAATCATCACAGAAGTGC
TGGCGGGGGCGTGGGAACGGCTGGAGCAGGGGCGGGCGGATATCGTTATCGCGCCGGATATGCATTTTCGTTCCTCGTCG
GAGATCAACTCGCGCAAGCTCTATACGTTAATGAACGTCTACGTTGCCGCGCCTGATCACCCGATTCATCAGGAGCCGGA
ACCGTTATCTGAAGTGACGCGTGTGAAATATCGTGGAATTGCGGTAGCGGATACCGCTCGTGAGCGCCCGGTGTTGACCG
TACAGCTGCTGGACAAACAGCCGCGCTTAACGGTGAGCACGATTGAAGATAAACGTCAGGCATTACTGGCGGGGCTTGGC
GTGGCGACGATGCCGTATCCGATGGTCGAAAAAGATATTGCGGAAGGGCGGTTGCGTGTCGTCAGCCCGGAATCGACCAG
CGAGATCGATATTATTATGGCCTGGCGTCGTGACAGTATGGGGGAAGCGAAATCCTGGTGTCTGCGGGAAATTCCAAAGT
TGTTCGCTGGAAAATAA

Upstream 100 bases:

>100_bases
CTTGACCTCTCTTTACTTCCTTTTATGGTAAGGGGCTGGACGCTACATTGTTAGCCAGATATTCTGCCCGGTATGTTCAA
ATTTCCTGAATGAGAACGAA

Downstream 100 bases:

>100_bases
TCTATTAAGCACCTTTCACAAGGTGCTTCTGACTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTA
CGCAGTCACTGCAATATATT

Product: putative LYSR-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEA
ADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK

Sequences:

>Translated_298_residues
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEA
ADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK
>Mature_297_residues
AKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAA
DKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSE
INSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGV
ATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789492, Length=298, Percent_Identity=99.6644295302013, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI87082024, Length=286, Percent_Identity=38.4615384615385, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1787949, Length=280, Percent_Identity=29.6428571428571, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786508, Length=296, Percent_Identity=29.3918918918919, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786713, Length=281, Percent_Identity=26.6903914590747, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33214; Mature: 33083

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHR
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCC
TKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLA
CHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDH
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEEECCCC
PIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCC
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK
HHHCCCHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHR
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCC
TKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLA
CHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDH
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEEECCCC
PIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCC
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK
HHHCCCHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]